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Article: A modified AFLP with fluorescence-labelled primers and automated DNA sequencer detection for efficient fingerprinting analysis in plants

TitleA modified AFLP with fluorescence-labelled primers and automated DNA sequencer detection for efficient fingerprinting analysis in plants
Authors
Huang, JSun, M
KeywordsAFLP
Amplified fragment length polymorphism
DNA fingerprinting
Issue Date1999
PublisherKluwer Academic Publishers. The Journal's web site is located at http://www.wkap.nl
Citation
Biotechnology Techniques, 1999, v. 13 n. 4, p. 277-278 How to Cite?
DOI: http://dx.doi.org/10.1023/A:1008970618252
AbstractA modified AFLP (amplified fragment length polymorphism) technique is described. Fluorescence-labelled primers were used in the selective amplifications. The amplified fragments were detected on denaturing polyacrylamide gels using an automated ALF DNA sequencer with the fragment option. The modified AFLP technique avoids the use of isotopes or silver staining, but gives a much higher resolution than other AFLP detection systems.
Persistent Identifierhttp://hdl.handle.net/10722/84915
ISSN
0951-208X
ISI Accession Number ID
WOS:000080544700013
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorHuang, Jen_HK
dc.contributor.authorSun, Men_HK
dc.date.accessioned2010-09-06T08:58:36Z-
dc.date.available2010-09-06T08:58:36Z-
dc.date.issued1999en_HK
dc.identifier.citationBiotechnology Techniques, 1999, v. 13 n. 4, p. 277-278en_HK
dc.identifier.issn0951-208Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84915-
dc.description.abstractA modified AFLP (amplified fragment length polymorphism) technique is described. Fluorescence-labelled primers were used in the selective amplifications. The amplified fragments were detected on denaturing polyacrylamide gels using an automated ALF DNA sequencer with the fragment option. The modified AFLP technique avoids the use of isotopes or silver staining, but gives a much higher resolution than other AFLP detection systems.en_HK
dc.languageengen_HK
dc.publisherKluwer Academic Publishers. The Journal's web site is located at http://www.wkap.nlen_HK
dc.relation.ispartofBiotechnology Techniquesen_HK
dc.rightsBiotechnology Techniques. Copyright © Kluwer Academic Publishers.en_HK
dc.subjectAFLPen_HK
dc.subjectAmplified fragment length polymorphismen_HK
dc.subjectDNA fingerprintingen_HK
dc.titleA modified AFLP with fluorescence-labelled primers and automated DNA sequencer detection for efficient fingerprinting analysis in plantsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0951-208X&volume=13&spage=277&epage=278&date=1999&atitle=A+modified+AFLP+with+fluorescence-labelled+primers+and+automated+DNA+sequencer+detection+for+efficient+fingerprinting+analysis+in+plantsen_HK
dc.identifier.emailHuang, J: huangjc@hku.hken_HK
dc.identifier.emailSun, M: meisun@hkucc.hku.hken_HK
dc.identifier.authorityHuang, J=rp00710en_HK
dc.identifier.authoritySun, M=rp00779en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1008970618252en_HK
dc.identifier.scopuseid_2-s2.0-0033005193en_HK
dc.identifier.hkuros44399en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033005193&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue4en_HK
dc.identifier.spage277en_HK
dc.identifier.epage278en_HK
dc.identifier.isiWOS:000080544700013-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHuang, J=7408108735en_HK
dc.identifier.scopusauthoridSun, M=7403181447en_HK
dc.identifier.issnl0951-208X-

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