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Article: Gastrointestinal stability and absorption of insulin in suckling pigs

TitleGastrointestinal stability and absorption of insulin in suckling pigs
Authors
KeywordsFluorescein-isothiocyanate labeled insulin (FITC-insulin)
Gastrointestinal (GI) tract
Intestinal absorption
Milk-borne insulin
Radioimmunoassay
Size-exclusion chromatography
Stability
Suckling pigs
Issue Date2000
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cbpa
Citation
Comparative Biochemistry And Physiology - A Molecular And Integrative Physiology, 2000, v. 125 n. 3, p. 389-401 How to Cite?
AbstractStability and absorption of orally administered fluorescein-isothiocyanate labeled insulin (FITC-insulin) in the gastrointestinal (GI) tract were investigated in newborn and 3-day-old pigs. The uptake of FITC-insulin by the intestinal epithelial cells was visualized using confocal laser scanning microscopy. Following oral administration, 3 h later 56 and 88% of orally administered fluorescence was found in the GI tract in newborn and 3-day-old piglets, respectively. Chromatographic analysis revealed that 15-37% of fluorescence recovered from the gastric and proximal intestinal contents was eluted in the void volume of a Sephadex G-25 column. It was also observed that oral administration of FITC-insulin at a dose of 100 nmol/kg body weight led to a significant decrease in blood glucose in newborn pigs (P<0.05) but not in 3-day-old pigs. Microscopic examination showed that FITC-insulin was taken up via the vesicular transport mechanism throughout the whole small intestine but the ileum appeared to be a preferred site for FITC-insulin transport in newborn pigs. In 3-day-old pigs, the uptake of FITC-insulin occurred only in the distal part of the small intestine. These findings suggest that milk-borne insulin may partially survive in the GI lumen and subsequently act on the gastrointestinal tract in suckling piglets, while GI absorption of milk-borne insulin is limited to newborn pigs. Copyright (C) 2000 Elsevier Science Inc.
Persistent Identifierhttp://hdl.handle.net/10722/84914
ISSN
2015 Impact Factor: 2.039
2015 SCImago Journal Rankings: 0.939
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShen, WHen_HK
dc.contributor.authorXu, RJen_HK
dc.date.accessioned2010-09-06T08:58:35Z-
dc.date.available2010-09-06T08:58:35Z-
dc.date.issued2000en_HK
dc.identifier.citationComparative Biochemistry And Physiology - A Molecular And Integrative Physiology, 2000, v. 125 n. 3, p. 389-401en_HK
dc.identifier.issn1095-6433en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84914-
dc.description.abstractStability and absorption of orally administered fluorescein-isothiocyanate labeled insulin (FITC-insulin) in the gastrointestinal (GI) tract were investigated in newborn and 3-day-old pigs. The uptake of FITC-insulin by the intestinal epithelial cells was visualized using confocal laser scanning microscopy. Following oral administration, 3 h later 56 and 88% of orally administered fluorescence was found in the GI tract in newborn and 3-day-old piglets, respectively. Chromatographic analysis revealed that 15-37% of fluorescence recovered from the gastric and proximal intestinal contents was eluted in the void volume of a Sephadex G-25 column. It was also observed that oral administration of FITC-insulin at a dose of 100 nmol/kg body weight led to a significant decrease in blood glucose in newborn pigs (P<0.05) but not in 3-day-old pigs. Microscopic examination showed that FITC-insulin was taken up via the vesicular transport mechanism throughout the whole small intestine but the ileum appeared to be a preferred site for FITC-insulin transport in newborn pigs. In 3-day-old pigs, the uptake of FITC-insulin occurred only in the distal part of the small intestine. These findings suggest that milk-borne insulin may partially survive in the GI lumen and subsequently act on the gastrointestinal tract in suckling piglets, while GI absorption of milk-borne insulin is limited to newborn pigs. Copyright (C) 2000 Elsevier Science Inc.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cbpaen_HK
dc.relation.ispartofComparative Biochemistry and Physiology - A Molecular and Integrative Physiologyen_HK
dc.rightsComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. Copyright © Elsevier Inc.en_HK
dc.subjectFluorescein-isothiocyanate labeled insulin (FITC-insulin)en_HK
dc.subjectGastrointestinal (GI) tracten_HK
dc.subjectIntestinal absorptionen_HK
dc.subjectMilk-borne insulinen_HK
dc.subjectRadioimmunoassayen_HK
dc.subjectSize-exclusion chromatographyen_HK
dc.subjectStabilityen_HK
dc.subjectSuckling pigsen_HK
dc.titleGastrointestinal stability and absorption of insulin in suckling pigsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1095-6433&volume=125A&spage=389&epage=401&date=2000&atitle=Gastrointestinal+stability+and+absorption+of+insulin+in+suckling+pigsen_HK
dc.identifier.emailXu, RJ: xuruojun@hkucc.hku.hken_HK
dc.identifier.authorityXu, RJ=rp00820en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1095-6433(00)00169-0en_HK
dc.identifier.pmid10794968-
dc.identifier.scopuseid_2-s2.0-0034011730en_HK
dc.identifier.hkuros48672en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034011730&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume125en_HK
dc.identifier.issue3en_HK
dc.identifier.spage389en_HK
dc.identifier.epage401en_HK
dc.identifier.isiWOS:000088269800011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShen, WH=46261460000en_HK
dc.identifier.scopusauthoridXu, RJ=7402813973en_HK

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