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Article: Retinoic acid activates human secretin gene expression by Sp proteins and Nuclear Factor I in neuronal SH-SY5Y cells

TitleRetinoic acid activates human secretin gene expression by Sp proteins and Nuclear Factor I in neuronal SH-SY5Y cells
Authors
KeywordsGene regulation
Human neuroblastoma cell
Nuclear Factor I
Retinoic acid
Secretin
SH-SY5Y
Sp1
Issue Date2005
PublisherBlackwell Publishing Ltd.
Citation
Journal Of Neurochemistry, 2005, v. 93 n. 2, p. 339-350 How to Cite?
AbstractSecretin is a neuropeptide that is expressed in distinct central neurones. As there is no information on how the secretin gene is regulated in neuronal cells, a well established neuronal differentiation cell model, SH-SY5Y, was used to study transcriptional regulation of the human secretin gene. High secretin transcript and peptide levels were found in this cell, and secretin gene expression and promoter activity were up-regulated upon all-trans retinoic acid (RA) treatment. Within the promoter, a functional GC-box 1 (-131 from ATG, relative to the ATG initiation codon) was found to be regulated by a brain-specific Sp protein, Sp4, and ubiquitous factors Sp1 and Sp3. The human secretin gene in SH-SY5Y cells is controlled by the (Sp1 + Sp4)/Sp3 ratio and the RA-induced activation is a partial result of a decrease in Sp3 levels. In addition to the GC-box 1, an N1 motif in close proximity was also responsible for RA-induced secretin gene activation. Competitive gel mobility shift and southwestern blot studies revealed binding of Nuclear Factor I (NFI) with the N1 motif. Overexpression of NFI-C increased promoter activity upon RA treatment. Consistent with this observation, NFI-C transcript levels were augmented after RA treatment. We conclude that RA induction of the secretin gene in neuronal cells is regulated by the combined actions of reducing Sp3 and increasing NFI-C expression. © 2005 International Society for Neurochemistry.
Persistent Identifierhttp://hdl.handle.net/10722/84913
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, LTOen_HK
dc.contributor.authorTanUn, KCen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:58:35Z-
dc.date.available2010-09-06T08:58:35Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Neurochemistry, 2005, v. 93 n. 2, p. 339-350en_HK
dc.identifier.issn0022-3042en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84913-
dc.description.abstractSecretin is a neuropeptide that is expressed in distinct central neurones. As there is no information on how the secretin gene is regulated in neuronal cells, a well established neuronal differentiation cell model, SH-SY5Y, was used to study transcriptional regulation of the human secretin gene. High secretin transcript and peptide levels were found in this cell, and secretin gene expression and promoter activity were up-regulated upon all-trans retinoic acid (RA) treatment. Within the promoter, a functional GC-box 1 (-131 from ATG, relative to the ATG initiation codon) was found to be regulated by a brain-specific Sp protein, Sp4, and ubiquitous factors Sp1 and Sp3. The human secretin gene in SH-SY5Y cells is controlled by the (Sp1 + Sp4)/Sp3 ratio and the RA-induced activation is a partial result of a decrease in Sp3 levels. In addition to the GC-box 1, an N1 motif in close proximity was also responsible for RA-induced secretin gene activation. Competitive gel mobility shift and southwestern blot studies revealed binding of Nuclear Factor I (NFI) with the N1 motif. Overexpression of NFI-C increased promoter activity upon RA treatment. Consistent with this observation, NFI-C transcript levels were augmented after RA treatment. We conclude that RA induction of the secretin gene in neuronal cells is regulated by the combined actions of reducing Sp3 and increasing NFI-C expression. © 2005 International Society for Neurochemistry.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd.en_HK
dc.relation.ispartofJournal of Neurochemistryen_HK
dc.rightsJournal of Neurochemistry. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectGene regulationen_HK
dc.subjectHuman neuroblastoma cellen_HK
dc.subjectNuclear Factor Ien_HK
dc.subjectRetinoic aciden_HK
dc.subjectSecretinen_HK
dc.subjectSH-SY5Yen_HK
dc.subjectSp1en_HK
dc.titleRetinoic acid activates human secretin gene expression by Sp proteins and Nuclear Factor I in neuronal SH-SY5Y cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3042&volume=93&spage=339&epage=350&date=2005&atitle=Retinoic+acid+activates+human+secretin+gene+expression+by+Sp+proteins+and+Nuclear+Factor+I+in+neuronal+SH-SY5Y+cellsen_HK
dc.identifier.emailLee, LTO: ltolee2@hkucc.hku.hken_HK
dc.identifier.emailTanUn, KC: kctanun@hkucc.hku.hken_HK
dc.identifier.emailLin, MCM: mcllin@hkucc.hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityLee, LTO=rp00727en_HK
dc.identifier.authorityTanUn, KC=rp00787en_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1471-4159.2005.03018.xen_HK
dc.identifier.pmid15816857-
dc.identifier.scopuseid_2-s2.0-17444416090en_HK
dc.identifier.hkuros98356en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-17444416090&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume93en_HK
dc.identifier.issue2en_HK
dc.identifier.spage339en_HK
dc.identifier.epage350en_HK
dc.identifier.isiWOS:000228017300009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLee, LTO=8367269000en_HK
dc.identifier.scopusauthoridTanUn, KC=6602914262en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK
dc.identifier.citeulike144595-

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