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Article: Beta-propeller phytases in the aquatic environment

TitleBeta-propeller phytases in the aquatic environment
Authors
KeywordsAquatic
Beta-propeller
Phosphorus
Phytase
Phytate
Shewanella oneidensis
Issue Date2006
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00203/index.htm
Citation
Archives Of Microbiology, 2006, v. 185 n. 1, p. 1-13 How to Cite?
AbstractPhytate, which is one of the dominant organic phosphorus compounds in nature, is very stable in soils. Although a substantial amount of phytate is carried from terrestrial to aquatic systems, it is a minor component of organic phosphorus in coastal sediments. The ephemeral nature of phytate implies the rapid hydrolysis of phytate under aquatic conditions. Among the four classes of known phytases that have been identified in terrestrial organisms, only β-propeller phytase-like sequences have been identified in the aquatic environment. A novel β-propeller phytase gene (phyS), cloned from Shewanella oneidensis MR-1, was found to encode a protein with two beta-propeller phytase domains. The characterization of recombinant full-length PhyS and its domains demonstrated that Domain II was the catalytic domain responsible for phytate hydrolysis. The full-length PhyS displayed a K m of 83 μM with a kcat of 175.9 min-1 and the Domain II displayed a Km of 474 μM with a kcat of 10.6 min-1. These results confirm that the phyS gene encodes a functional β-propeller phytase, which is expressed in S. oneidensis under phosphorus deficienct condition. The presence of multiple sequences with a high similarity to phyS in aquatic environmental samples and the widespread occurrence of the Shewanella species in nature suggest that the β-propeller phytase family is the major class of phytases in the aquatic environment, and that it may play an important role in the recycling of phosphorus. © Springer-Verlag 2006.
Persistent Identifierhttp://hdl.handle.net/10722/84911
ISSN
2015 Impact Factor: 1.76
2015 SCImago Journal Rankings: 0.702
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheng, Cen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2010-09-06T08:58:33Z-
dc.date.available2010-09-06T08:58:33Z-
dc.date.issued2006en_HK
dc.identifier.citationArchives Of Microbiology, 2006, v. 185 n. 1, p. 1-13en_HK
dc.identifier.issn0302-8933en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84911-
dc.description.abstractPhytate, which is one of the dominant organic phosphorus compounds in nature, is very stable in soils. Although a substantial amount of phytate is carried from terrestrial to aquatic systems, it is a minor component of organic phosphorus in coastal sediments. The ephemeral nature of phytate implies the rapid hydrolysis of phytate under aquatic conditions. Among the four classes of known phytases that have been identified in terrestrial organisms, only β-propeller phytase-like sequences have been identified in the aquatic environment. A novel β-propeller phytase gene (phyS), cloned from Shewanella oneidensis MR-1, was found to encode a protein with two beta-propeller phytase domains. The characterization of recombinant full-length PhyS and its domains demonstrated that Domain II was the catalytic domain responsible for phytate hydrolysis. The full-length PhyS displayed a K m of 83 μM with a kcat of 175.9 min-1 and the Domain II displayed a Km of 474 μM with a kcat of 10.6 min-1. These results confirm that the phyS gene encodes a functional β-propeller phytase, which is expressed in S. oneidensis under phosphorus deficienct condition. The presence of multiple sequences with a high similarity to phyS in aquatic environmental samples and the widespread occurrence of the Shewanella species in nature suggest that the β-propeller phytase family is the major class of phytases in the aquatic environment, and that it may play an important role in the recycling of phosphorus. © Springer-Verlag 2006.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00203/index.htmen_HK
dc.relation.ispartofArchives of Microbiologyen_HK
dc.subjectAquaticen_HK
dc.subjectBeta-propelleren_HK
dc.subjectPhosphorusen_HK
dc.subjectPhytaseen_HK
dc.subjectPhytateen_HK
dc.subjectShewanella oneidensisen_HK
dc.titleBeta-propeller phytases in the aquatic environmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0302-8933&volume=185&spage=1&epage=13&date=2005&atitle=Beta-propeller+phytases+in+the+aquatic+environmenten_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00203-005-0080-6en_HK
dc.identifier.pmid16402222-
dc.identifier.scopuseid_2-s2.0-33644535130en_HK
dc.identifier.hkuros103190en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644535130&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume185en_HK
dc.identifier.issue1en_HK
dc.identifier.spage1en_HK
dc.identifier.epage13en_HK
dc.identifier.isiWOS:000235570200001-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridCheng, C=7404797223en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK

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