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Article: Characterization of an additional molt inhibiting hormone-like neuropeptide from the shrimp Metapenaeus ensis

TitleCharacterization of an additional molt inhibiting hormone-like neuropeptide from the shrimp Metapenaeus ensis
Authors
KeywordsEyestalk
Gene organization
Molt inhibiting hormone
Shrimp reproduction
Issue Date2002
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/peptides
Citation
Peptides, 2002, v. 23 n. 11, p. 1875-1883 How to Cite?
AbstractWe have identified a second form of the type-II neuropeptide encoding a molt inhibiting hormone-like (MeeMIH-B) neuropeptide. MeeMIH-B showed only a 70% amino acid identity to the MIH-A (formerly MIH) isolated from the same species, suggesting a possible different function of the deduced neuropeptide. Like other neuropeptide members of the CHH family, the MIH-B gene consists of three exons separated by two introns. The levels of MIH-B mRNA transcript in the eyestalk decrease in the initial phase of gonad maturation and increase towards the end of maturation. The drop in MIH-B level suggests an inhibitory role for this neuropeptide in the initiation of vitellogenesis. MIH-B transcripts can also be detected in the brain, thoracic ganglion and ventral nerve cord. Together with the CHH-B peptide that we have previously described, this is the second peptide of the CHH family that can also be identified in the ventral nerve cord and in the XOSG complex. A recombinant MIH-B was produced and a polyclonal antibody against rMIH-B was subsequently generated. Specific anti-rMIH-B antiserum recognized the presence of MIH-B in the sinus gland, X-organs, as well as a giant neuron of the ventral nerve cord. Injection of rMIH-B delayed the molting cycle of the maturing female. Taken together, the results of this study suggest that a drop in MIH-B level may be required for the delay in the molting of the maturing females. © 2002 Elsevier Science Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/84910
ISSN
2015 Impact Factor: 2.535
2015 SCImago Journal Rankings: 1.128
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGu, PLen_HK
dc.contributor.authorTobe, SSen_HK
dc.contributor.authorChow, BKCen_HK
dc.contributor.authorChu, KHen_HK
dc.contributor.authorHe, JGen_HK
dc.contributor.authorChan, SMen_HK
dc.date.accessioned2010-09-06T08:58:33Z-
dc.date.available2010-09-06T08:58:33Z-
dc.date.issued2002en_HK
dc.identifier.citationPeptides, 2002, v. 23 n. 11, p. 1875-1883en_HK
dc.identifier.issn0196-9781en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84910-
dc.description.abstractWe have identified a second form of the type-II neuropeptide encoding a molt inhibiting hormone-like (MeeMIH-B) neuropeptide. MeeMIH-B showed only a 70% amino acid identity to the MIH-A (formerly MIH) isolated from the same species, suggesting a possible different function of the deduced neuropeptide. Like other neuropeptide members of the CHH family, the MIH-B gene consists of three exons separated by two introns. The levels of MIH-B mRNA transcript in the eyestalk decrease in the initial phase of gonad maturation and increase towards the end of maturation. The drop in MIH-B level suggests an inhibitory role for this neuropeptide in the initiation of vitellogenesis. MIH-B transcripts can also be detected in the brain, thoracic ganglion and ventral nerve cord. Together with the CHH-B peptide that we have previously described, this is the second peptide of the CHH family that can also be identified in the ventral nerve cord and in the XOSG complex. A recombinant MIH-B was produced and a polyclonal antibody against rMIH-B was subsequently generated. Specific anti-rMIH-B antiserum recognized the presence of MIH-B in the sinus gland, X-organs, as well as a giant neuron of the ventral nerve cord. Injection of rMIH-B delayed the molting cycle of the maturing female. Taken together, the results of this study suggest that a drop in MIH-B level may be required for the delay in the molting of the maturing females. © 2002 Elsevier Science Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/peptidesen_HK
dc.relation.ispartofPeptidesen_HK
dc.rightsPeptides. Copyright © Elsevier BV.en_HK
dc.subjectEyestalken_HK
dc.subjectGene organizationen_HK
dc.subjectMolt inhibiting hormoneen_HK
dc.subjectShrimp reproductionen_HK
dc.titleCharacterization of an additional molt inhibiting hormone-like neuropeptide from the shrimp Metapenaeus ensisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0079-0753&volume=23&spage=1875&epage=1883&date=2002&atitle=Characterization+of+an+additional+molt+inhibiting+hormone-like+neuropeptide+from+the+shrimp+Metapenaeus+ensisen_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0196-9781(02)00178-Xen_HK
dc.identifier.scopuseid_2-s2.0-0036850691en_HK
dc.identifier.hkuros75605en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036850691&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1875en_HK
dc.identifier.epage1883en_HK
dc.identifier.isiWOS:000179602900002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGu, PL=8943600800en_HK
dc.identifier.scopusauthoridTobe, SS=7102112085en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK
dc.identifier.scopusauthoridChu, KH=7402453508en_HK
dc.identifier.scopusauthoridHe, JG=35487616500en_HK
dc.identifier.scopusauthoridChan, SM=7404255669en_HK

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