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Article: Cloning and sequencing of a cDNA encoding chicken mannan-binding lectin (MBL) and comparison with mammalian analogues

TitleCloning and sequencing of a cDNA encoding chicken mannan-binding lectin (MBL) and comparison with mammalian analogues
Authors
Issue Date1998
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMM
Citation
Immunology, 1998, v. 93 n. 3, p. 421-430 How to Cite?
AbstractThe serum lectin, mannan-binding lectin (MBL) (also denoted mannan- binding protein or mannose-binding protein, MBP) has been identified in mammals (humans, monkey, cow, rabbit, mouse and rat). Upon binding to carbohydrates on the surface of microorganisms, MBL mediates activation of the complement system, leading to killing of the microorganism. MBL thus exerts a role in the innate immune defence. We have described the isolation and partial characterization of an analogous protein in chicken serum. Oligonucleotides based on the N-terminal sequence of this protein were used in a reverse transcription-polymerase chain reaction (RT-PCR) with chicken liver RNA as template. The PCR product was sequenced and found to encode part of the NH2 terminus of chicken MBL. A perfect match probe was synthesized and used to screen a chicken liver cDNA library. The isolated clones carried a cDNA insert of 1692 bp with an open reading frame of 714 bp encoding a mature protein of 238 amino acids including a signal peptide of five amino acids. The deduced amino acid sequence agrees with those determined by conventional amino acid sequence analysis of the peptides except for four residues. We have compared the deduced primary structure of chicken MBL with the mammalian analogues. The phylogenetic analysis indicates that the gene duplication leading to two different MBL forms in mammals occurred after the split from birds and reptiles. This concurs with the finding of only one form of MBL in chickens.
Persistent Identifierhttp://hdl.handle.net/10722/84905
ISSN
2015 Impact Factor: 4.078
2015 SCImago Journal Rankings: 2.038
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLaursen, SBen_HK
dc.contributor.authorDalgaard, TSen_HK
dc.contributor.authorThiel, Sen_HK
dc.contributor.authorLim, BLen_HK
dc.contributor.authorJensen, TVen_HK
dc.contributor.authorJuulMadsen, HRen_HK
dc.contributor.authorTakahashi, Aen_HK
dc.contributor.authorHamana, Ten_HK
dc.contributor.authorKawakami, Men_HK
dc.contributor.authorJensenius, JCen_HK
dc.date.accessioned2010-09-06T08:58:29Z-
dc.date.available2010-09-06T08:58:29Z-
dc.date.issued1998en_HK
dc.identifier.citationImmunology, 1998, v. 93 n. 3, p. 421-430en_HK
dc.identifier.issn0019-2805en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84905-
dc.description.abstractThe serum lectin, mannan-binding lectin (MBL) (also denoted mannan- binding protein or mannose-binding protein, MBP) has been identified in mammals (humans, monkey, cow, rabbit, mouse and rat). Upon binding to carbohydrates on the surface of microorganisms, MBL mediates activation of the complement system, leading to killing of the microorganism. MBL thus exerts a role in the innate immune defence. We have described the isolation and partial characterization of an analogous protein in chicken serum. Oligonucleotides based on the N-terminal sequence of this protein were used in a reverse transcription-polymerase chain reaction (RT-PCR) with chicken liver RNA as template. The PCR product was sequenced and found to encode part of the NH2 terminus of chicken MBL. A perfect match probe was synthesized and used to screen a chicken liver cDNA library. The isolated clones carried a cDNA insert of 1692 bp with an open reading frame of 714 bp encoding a mature protein of 238 amino acids including a signal peptide of five amino acids. The deduced amino acid sequence agrees with those determined by conventional amino acid sequence analysis of the peptides except for four residues. We have compared the deduced primary structure of chicken MBL with the mammalian analogues. The phylogenetic analysis indicates that the gene duplication leading to two different MBL forms in mammals occurred after the split from birds and reptiles. This concurs with the finding of only one form of MBL in chickens.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMMen_HK
dc.relation.ispartofImmunologyen_HK
dc.rightsImmunology. Copyright © Blackwell Publishing Ltd.en_HK
dc.titleCloning and sequencing of a cDNA encoding chicken mannan-binding lectin (MBL) and comparison with mammalian analoguesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0019-2805&volume=93&spage=421&epage=430&date=1998&atitle=Cloning+and+sequencing+of+a+cDNA+encoding+chicken+mannan-binding+lectin+(MBL)+and+comparison+with+mammalian+analoguesen_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-2567.1998.00446.xen_HK
dc.identifier.pmid9640255-
dc.identifier.scopuseid_2-s2.0-15644369627en_HK
dc.identifier.hkuros34157en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-15644369627&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume93en_HK
dc.identifier.issue3en_HK
dc.identifier.spage421en_HK
dc.identifier.epage430en_HK
dc.identifier.isiWOS:000073218500017-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLaursen, SB=7005289302en_HK
dc.identifier.scopusauthoridDalgaard, TS=35097171500en_HK
dc.identifier.scopusauthoridThiel, S=7004524621en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK
dc.identifier.scopusauthoridJensen, TV=7401539716en_HK
dc.identifier.scopusauthoridJuulMadsen, HR=6603714814en_HK
dc.identifier.scopusauthoridTakahashi, A=7402799352en_HK
dc.identifier.scopusauthoridHamana, T=19234169300en_HK
dc.identifier.scopusauthoridKawakami, M=7402546340en_HK
dc.identifier.scopusauthoridJensenius, JC=7005603928en_HK

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