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Article: Assessment of genetic, antigenic and pathotypic criteria for the characterization of IBDV strains

TitleAssessment of genetic, antigenic and pathotypic criteria for the characterization of IBDV strains
Authors
Issue Date2004
PublisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/03079457.asp
Citation
Avian Pathology, 2004, v. 33 n. 5, p. 470-476 How to Cite?
AbstractThe aim of this work was the selection and comparison of representative infectious bursal disease virus (IBDV) strains. Nine strains of IBDV, isolated at different times and from different geographic regions of Europe and China, were characterized. Batches of all strains were prepared following standardized protocols and checked for the absence of contaminating viruses. Criteria used for their characterization were: (i) the nucleotide sequence of the VP2 variable region, (ii) binding to a panel of neutralizing monoclonal antibodies in antigen capture enzyme-linked immunosorbent assays, and (iii) virulence in specific pathogen free chickens after infection with a standardized number of median embryo infective doses. Based on the first two criteria, two of nine strains were classified as classical virulent (cv) IBDV (F52/70, Cu-1wt), and five as very virulent (vv) IBDV (849VB, 96108, HK46, GX, Harbin). Remarkably, although a clear-cut difference was demonstrable between European cvIBDV (F52/70 and Cu-1wt) and vvIBDV (849VB and 96108) strains, there was a continuum in the pathogenicity of Chinese vvIBDVs. Our results indicate the probable existence of differences in virulence within IBDV lineages determined on the basis of antigenic typing using monoclonal antibodies and the alignment of the VP2 sequences. This indicates limitations in the analysis of IBDV pathotypes based on the VP2 variable region and emphasizes that these criteria may not be sufficient for the classification of IBDV strains.
Persistent Identifierhttp://hdl.handle.net/10722/84900
ISSN
2015 Impact Factor: 1.336
2015 SCImago Journal Rankings: 0.918
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorVan Den Berg, TPen_HK
dc.contributor.authorMorales, Den_HK
dc.contributor.authorEterradossi, Nen_HK
dc.contributor.authorRivallan, Gen_HK
dc.contributor.authorToquin, Den_HK
dc.contributor.authorRaue, Ren_HK
dc.contributor.authorZierenberg, Ken_HK
dc.contributor.authorZhang, MFen_HK
dc.contributor.authorZhu, YPen_HK
dc.contributor.authorWang, CQen_HK
dc.contributor.authorZheng, HJen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorChen, GCen_HK
dc.contributor.authorLim, BLen_HK
dc.contributor.authorMüller, Hen_HK
dc.date.accessioned2010-09-06T08:58:25Z-
dc.date.available2010-09-06T08:58:25Z-
dc.date.issued2004en_HK
dc.identifier.citationAvian Pathology, 2004, v. 33 n. 5, p. 470-476en_HK
dc.identifier.issn0307-9457en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84900-
dc.description.abstractThe aim of this work was the selection and comparison of representative infectious bursal disease virus (IBDV) strains. Nine strains of IBDV, isolated at different times and from different geographic regions of Europe and China, were characterized. Batches of all strains were prepared following standardized protocols and checked for the absence of contaminating viruses. Criteria used for their characterization were: (i) the nucleotide sequence of the VP2 variable region, (ii) binding to a panel of neutralizing monoclonal antibodies in antigen capture enzyme-linked immunosorbent assays, and (iii) virulence in specific pathogen free chickens after infection with a standardized number of median embryo infective doses. Based on the first two criteria, two of nine strains were classified as classical virulent (cv) IBDV (F52/70, Cu-1wt), and five as very virulent (vv) IBDV (849VB, 96108, HK46, GX, Harbin). Remarkably, although a clear-cut difference was demonstrable between European cvIBDV (F52/70 and Cu-1wt) and vvIBDV (849VB and 96108) strains, there was a continuum in the pathogenicity of Chinese vvIBDVs. Our results indicate the probable existence of differences in virulence within IBDV lineages determined on the basis of antigenic typing using monoclonal antibodies and the alignment of the VP2 sequences. This indicates limitations in the analysis of IBDV pathotypes based on the VP2 variable region and emphasizes that these criteria may not be sufficient for the classification of IBDV strains.en_HK
dc.languageengen_HK
dc.publisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/03079457.aspen_HK
dc.relation.ispartofAvian Pathologyen_HK
dc.titleAssessment of genetic, antigenic and pathotypic criteria for the characterization of IBDV strainsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0307-9457&volume=33&spage=470&epage=476&date=2004&atitle=Assessment+of+genetic,+antigenic+and+pathotypic+criteria+for+the+characterization+of+IBDV+strainsen_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/03079450400003650en_HK
dc.identifier.pmid15545026-
dc.identifier.scopuseid_2-s2.0-8644229493en_HK
dc.identifier.hkuros97438en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-8644229493&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume33en_HK
dc.identifier.issue5en_HK
dc.identifier.spage470en_HK
dc.identifier.epage476en_HK
dc.identifier.isiWOS:000224820900002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridVan Den Berg, TP=7006543741en_HK
dc.identifier.scopusauthoridMorales, D=36965913800en_HK
dc.identifier.scopusauthoridEterradossi, N=7003674878en_HK
dc.identifier.scopusauthoridRivallan, G=16639915000en_HK
dc.identifier.scopusauthoridToquin, D=6701342017en_HK
dc.identifier.scopusauthoridRaue, R=7004050426en_HK
dc.identifier.scopusauthoridZierenberg, K=15851895900en_HK
dc.identifier.scopusauthoridZhang, MF=7601556974en_HK
dc.identifier.scopusauthoridZhu, YP=9248689600en_HK
dc.identifier.scopusauthoridWang, CQ=7501632511en_HK
dc.identifier.scopusauthoridZheng, HJ=7403441302en_HK
dc.identifier.scopusauthoridWang, X=23037123800en_HK
dc.identifier.scopusauthoridChen, GC=7406544487en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK
dc.identifier.scopusauthoridMüller, H=7404944353en_HK

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