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Article: The goldfish vasoactive intestinal polypeptide receptor: Functional studies and tissue distribution

TitleThe goldfish vasoactive intestinal polypeptide receptor: Functional studies and tissue distribution
Authors
KeywordsAdenylyl cyclase
cAMP
G-protein coupled receptor
Goldfish
PACAP
Secretin
Vasoactive intestinal polypeptide
Issue Date1997
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-1742
Citation
Fish Physiology And Biochemistry, 1997, v. 17 n. 1-6, p. 213-222 How to Cite?
AbstractVasoactive intestinal polypeptide (VIP)1 is a neuropeptide that has numerous physiological actions and is widely distibuted in the body of most vertebrate species yet studied. A full-length VIP receptor cDNA was obtained by screening a goldfish brain and pituitary cDNA library. Functional expression of this receptor in mammalian COS-7 cells showed that it is coupled to cAMP production in a VFP (Atlantic cod, Gadus morhua) concentration-dependent manner; the EC50 of the VIP receptor in response to cod VIP stimulation was determined to be at 1.0 nM. At 100 nM of peptide, the relative potency of various peptides in stimulating cAMP in the transfected cells was cod VIP>human VIP>human PACAP-38>human PACAP-27>human secretin>dogfish glucagon>carp GHRH. The receptor was able to bind specifically to VIP and PACAP as indicated by competitive displacement assays. By reverse-transcriptase polymerase chain reaction, the transcripts of this receptor were detected in goldfish brain, pituitary gland, gall bladder, kidney, gill, small and large intestine, heart, muscle and spleen. The expression pattern of goldfish VIP receptor is similar to that of mammals and thus further evidence of the conservation of VIP function in vertebrates. Characterization of the VFP1 receptor from goldfish is the first step in understanding 2 of the most important neuropeptides, VIP and PACAP, and their interaction with multiple receptor system in lower vertebrates.
Persistent Identifierhttp://hdl.handle.net/10722/84880
ISSN
2015 Impact Factor: 1.442
2015 SCImago Journal Rankings: 0.738
References

 

DC FieldValueLanguage
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:58:11Z-
dc.date.available2010-09-06T08:58:11Z-
dc.date.issued1997en_HK
dc.identifier.citationFish Physiology And Biochemistry, 1997, v. 17 n. 1-6, p. 213-222en_HK
dc.identifier.issn0920-1742en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84880-
dc.description.abstractVasoactive intestinal polypeptide (VIP)1 is a neuropeptide that has numerous physiological actions and is widely distibuted in the body of most vertebrate species yet studied. A full-length VIP receptor cDNA was obtained by screening a goldfish brain and pituitary cDNA library. Functional expression of this receptor in mammalian COS-7 cells showed that it is coupled to cAMP production in a VFP (Atlantic cod, Gadus morhua) concentration-dependent manner; the EC50 of the VIP receptor in response to cod VIP stimulation was determined to be at 1.0 nM. At 100 nM of peptide, the relative potency of various peptides in stimulating cAMP in the transfected cells was cod VIP>human VIP>human PACAP-38>human PACAP-27>human secretin>dogfish glucagon>carp GHRH. The receptor was able to bind specifically to VIP and PACAP as indicated by competitive displacement assays. By reverse-transcriptase polymerase chain reaction, the transcripts of this receptor were detected in goldfish brain, pituitary gland, gall bladder, kidney, gill, small and large intestine, heart, muscle and spleen. The expression pattern of goldfish VIP receptor is similar to that of mammals and thus further evidence of the conservation of VIP function in vertebrates. Characterization of the VFP1 receptor from goldfish is the first step in understanding 2 of the most important neuropeptides, VIP and PACAP, and their interaction with multiple receptor system in lower vertebrates.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-1742en_HK
dc.relation.ispartofFish Physiology and Biochemistryen_HK
dc.subjectAdenylyl cyclaseen_HK
dc.subjectcAMPen_HK
dc.subjectG-protein coupled receptoren_HK
dc.subjectGoldfishen_HK
dc.subjectPACAPen_HK
dc.subjectSecretinen_HK
dc.subjectVasoactive intestinal polypeptideen_HK
dc.titleThe goldfish vasoactive intestinal polypeptide receptor: Functional studies and tissue distributionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0920-1742&volume=17&spage=213&epage=222&date=1997&atitle=The+goldfish+vasoactive+intestinal+polypeptide+receptor:+functional+studies+and+tissue+distributionen_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0000521635en_HK
dc.identifier.hkuros24438en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0000521635&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue1-6en_HK
dc.identifier.spage213en_HK
dc.identifier.epage222en_HK
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK

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