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Article: Epithelio-mesenchymal transition in a neoplastic ovarian epithelial hybrid cell line

TitleEpithelio-mesenchymal transition in a neoplastic ovarian epithelial hybrid cell line
Authors
KeywordsDifferentiation
EMT
Epithelio-mesenchymal transition
Hybrid
Ovarian cancer
Issue Date2004
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/DIF
Citation
Differentiation, 2004, v. 72 n. 4, p. 150-161 How to Cite?
AbstractA hybrid cell line, IOSE-Ov29, was created through fusion of cells from the human ovarian adenocarcinoma line OVCAR3 and the non-tumorigenic SV40 Tag-transfected human ovarian surface epithelial line IOSE-29. OVCAR3 cells exhibit a differentiated epithelial phenotype, whereas line IOSE-29 expresses mesenchymal characteristics that were acquired in culture by epithelio-mesenchymal transition. Microsatellite analysis, comparative genomic hybridization (CGH), and MFISH showed the genotype of the IOSE-Ov29 cells to contain components of both parent cell lines, but to be predominantly OVCAR3 derived. IOSE-Ov29 resembled OVCAR3 and differed from IOSE-29 as shown by its unlimited life span, tumorigenicity, epithelial morphology, keratin, occludin, E-cadherin and CA125 expression, increased expression of kinases of the PI3K pathway, and loss of cGMP-dependent protein kinase expression. IOSE-29-derived properties included SV40 Tag expression, growth inhibition by activin, collagen type III secretion, increased adhesion and spreading on tissue culture plastic, and increased growth rate. Proliferation of all three lines was stimulated by FSH and ATP and inhibited by GnRH I and GnRH II. Interestingly, IOSE-Ov29 was more anchorage independent than either parent line and was the only line that invaded Matrigel in Boyden chambers and formed invasive branches in collagen gels. The results indicate that IOSE-Ov29 is an IOSE-29/OVCAR3 hybrid, which differs from both parent lines genetically and phenotypically. Unexpectedly, fusion with the non-tumorigenic IOSE-29 cells enhanced malignancy-associated characteristics of OVCAR3, presumably as a result of the expression of IOSE-29-derived mesenchymal properties that are usually acquired by carcinoma cells through epithelio-mesenchymal transition during metastatic progression. © International Society of Differentiation 2004.
Persistent Identifierhttp://hdl.handle.net/10722/84871
ISSN
2015 Impact Factor: 2.461
2015 SCImago Journal Rankings: 1.747
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMainesBandiera, SLen_HK
dc.contributor.authorHuntsman, Den_HK
dc.contributor.authorLestou, VSen_HK
dc.contributor.authorKuo, WLen_HK
dc.contributor.authorLeung, PCKen_HK
dc.contributor.authorHorsman, RDen_HK
dc.contributor.authorWong, ASTen_HK
dc.contributor.authorWoo, MMMen_HK
dc.contributor.authorChoi, KKCen_HK
dc.contributor.authorRoskelley, CDen_HK
dc.contributor.authorAuersperg, Nen_HK
dc.date.accessioned2010-09-06T08:58:05Z-
dc.date.available2010-09-06T08:58:05Z-
dc.date.issued2004en_HK
dc.identifier.citationDifferentiation, 2004, v. 72 n. 4, p. 150-161en_HK
dc.identifier.issn0301-4681en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84871-
dc.description.abstractA hybrid cell line, IOSE-Ov29, was created through fusion of cells from the human ovarian adenocarcinoma line OVCAR3 and the non-tumorigenic SV40 Tag-transfected human ovarian surface epithelial line IOSE-29. OVCAR3 cells exhibit a differentiated epithelial phenotype, whereas line IOSE-29 expresses mesenchymal characteristics that were acquired in culture by epithelio-mesenchymal transition. Microsatellite analysis, comparative genomic hybridization (CGH), and MFISH showed the genotype of the IOSE-Ov29 cells to contain components of both parent cell lines, but to be predominantly OVCAR3 derived. IOSE-Ov29 resembled OVCAR3 and differed from IOSE-29 as shown by its unlimited life span, tumorigenicity, epithelial morphology, keratin, occludin, E-cadherin and CA125 expression, increased expression of kinases of the PI3K pathway, and loss of cGMP-dependent protein kinase expression. IOSE-29-derived properties included SV40 Tag expression, growth inhibition by activin, collagen type III secretion, increased adhesion and spreading on tissue culture plastic, and increased growth rate. Proliferation of all three lines was stimulated by FSH and ATP and inhibited by GnRH I and GnRH II. Interestingly, IOSE-Ov29 was more anchorage independent than either parent line and was the only line that invaded Matrigel in Boyden chambers and formed invasive branches in collagen gels. The results indicate that IOSE-Ov29 is an IOSE-29/OVCAR3 hybrid, which differs from both parent lines genetically and phenotypically. Unexpectedly, fusion with the non-tumorigenic IOSE-29 cells enhanced malignancy-associated characteristics of OVCAR3, presumably as a result of the expression of IOSE-29-derived mesenchymal properties that are usually acquired by carcinoma cells through epithelio-mesenchymal transition during metastatic progression. © International Society of Differentiation 2004.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/DIFen_HK
dc.relation.ispartofDifferentiationen_HK
dc.rightsDifferentiation. Copyright © Elsevier Ltd.en_HK
dc.subjectDifferentiationen_HK
dc.subjectEMTen_HK
dc.subjectEpithelio-mesenchymal transitionen_HK
dc.subjectHybriden_HK
dc.subjectOvarian canceren_HK
dc.titleEpithelio-mesenchymal transition in a neoplastic ovarian epithelial hybrid cell lineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-4681&volume=72&spage=150&epage=161&date=2004&atitle=Epithelio-mesenchymal+transition+in+a+neoplastic+ovarian+epithelial+hybrid+cell+lineen_HK
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_HK
dc.identifier.authorityWong, AST=rp00805en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1432-0436.2004.07204003.xen_HK
dc.identifier.scopuseid_2-s2.0-4043167126en_HK
dc.identifier.hkuros91123en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4043167126&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume72en_HK
dc.identifier.issue4en_HK
dc.identifier.spage150en_HK
dc.identifier.epage161en_HK
dc.identifier.isiWOS:000221545300004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridMainesBandiera, SL=6602971815en_HK
dc.identifier.scopusauthoridHuntsman, D=7003437964en_HK
dc.identifier.scopusauthoridLestou, VS=6701456345en_HK
dc.identifier.scopusauthoridKuo, WL=7202113195en_HK
dc.identifier.scopusauthoridLeung, PCK=55419381000en_HK
dc.identifier.scopusauthoridHorsman, RD=9637666600en_HK
dc.identifier.scopusauthoridWong, AST=23987963300en_HK
dc.identifier.scopusauthoridWoo, MMM=7201527679en_HK
dc.identifier.scopusauthoridChoi, KKC=9634550600en_HK
dc.identifier.scopusauthoridRoskelley, CD=7004526388en_HK
dc.identifier.scopusauthoridAuersperg, N=7006582556en_HK

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