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Article: Consensus multiplex PCR-restriction fragment length polymorphism (RFLP) for rapid detection of plant mitochondrial DNA polymorphism

TitleConsensus multiplex PCR-restriction fragment length polymorphism (RFLP) for rapid detection of plant mitochondrial DNA polymorphism
Authors
KeywordsConsensus organelle primers
Mitochondrial DNA
Multiplex PCR
RFLP
Spiranthes
Issue Date1998
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MEC
Citation
Molecular Ecology, 1998, v. 7 n. 11, p. 1553-1556 How to Cite?
AbstractA fast, simple, and efficient approach, termed consensus multiplex PCR-RFLP, was developed and employed to detect mitochondrial (mt)DNA variation in three orchid species, Spiranthes hongkongensis, S. sinensis, and S. spiralis. Using multiplex PCR, three pairs of consensus mitochondrial primers were added simultaneously into each reaction tube to amplify three nonoverlapping introns located in the NADH dehydrogenase genes. Fragment length differences in the multiplex PCR amplicons were directly detectable between S. spiralis and the other two species. Further restriction analysis of the multiplex PCR amplicons revealed sufficient mtDNA polymorphism, suitable for phylogenetic studies at the interspecific level. This approach is well suited for large-scale population surveys of mitochondrial genome diversity in plants. Additionally, the maternal mode of inheritance of organelle genomes renders this approach valuable for rapid identification of the origin and specific parentage of hybrid or allopolyploid species.
Persistent Identifierhttp://hdl.handle.net/10722/84862
ISSN
2015 Impact Factor: 5.947
2015 SCImago Journal Rankings: 3.925
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Hen_HK
dc.contributor.authorSun, Men_HK
dc.date.accessioned2010-09-06T08:57:59Z-
dc.date.available2010-09-06T08:57:59Z-
dc.date.issued1998en_HK
dc.identifier.citationMolecular Ecology, 1998, v. 7 n. 11, p. 1553-1556en_HK
dc.identifier.issn0962-1083en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84862-
dc.description.abstractA fast, simple, and efficient approach, termed consensus multiplex PCR-RFLP, was developed and employed to detect mitochondrial (mt)DNA variation in three orchid species, Spiranthes hongkongensis, S. sinensis, and S. spiralis. Using multiplex PCR, three pairs of consensus mitochondrial primers were added simultaneously into each reaction tube to amplify three nonoverlapping introns located in the NADH dehydrogenase genes. Fragment length differences in the multiplex PCR amplicons were directly detectable between S. spiralis and the other two species. Further restriction analysis of the multiplex PCR amplicons revealed sufficient mtDNA polymorphism, suitable for phylogenetic studies at the interspecific level. This approach is well suited for large-scale population surveys of mitochondrial genome diversity in plants. Additionally, the maternal mode of inheritance of organelle genomes renders this approach valuable for rapid identification of the origin and specific parentage of hybrid or allopolyploid species.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MECen_HK
dc.relation.ispartofMolecular Ecologyen_HK
dc.rightsMolecular Ecology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectConsensus organelle primersen_HK
dc.subjectMitochondrial DNAen_HK
dc.subjectMultiplex PCRen_HK
dc.subjectRFLPen_HK
dc.subjectSpiranthesen_HK
dc.titleConsensus multiplex PCR-restriction fragment length polymorphism (RFLP) for rapid detection of plant mitochondrial DNA polymorphismen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0962-1083&volume=7&spage=1553&epage=1556&date=1998&atitle=Consensus+multiplex+PCR-restriction+fragment+length+polymorphism+(RFLP)+for+rapid+detection+of+plant+mitochondrial+DNA+polymorphismen_HK
dc.identifier.emailSun, M: meisun@hkucc.hku.hken_HK
dc.identifier.authoritySun, M=rp00779en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-294x.1998.00476.xen_HK
dc.identifier.scopuseid_2-s2.0-0031729495en_HK
dc.identifier.hkuros39458en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031729495&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1553en_HK
dc.identifier.epage1556en_HK
dc.identifier.isiWOS:000076933000012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChen, H=7501612240en_HK
dc.identifier.scopusauthoridSun, M=7403181447en_HK

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