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Article: Morphological identification of live gonadotropin, growth-hormone and prolactin cells in goldfish (Carassius auratus) pituitary-cell cultures

TitleMorphological identification of live gonadotropin, growth-hormone and prolactin cells in goldfish (Carassius auratus) pituitary-cell cultures
Authors
KeywordsCell identification
Cell separation
Differential interference-contrast (Nomarski) microscopy
Gonadotropin cells
Growth-hormone cells
Immunofluorescence
Prolactin cells
Teleostei goldfish
Issue Date1994
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
Citation
Cell And Tissue Research, 1994, v. 276 n. 2, p. 253-261 How to Cite?
AbstractTo better understand neuroendocrine regulation and the intracellular mechanisms mediating pituitary-hormone release, it is necessary to study the physiology of identified single cells. We have developed a system to identify gonadotropin, growth-hormone, and prolactin cells in primary cultures of goldfish pituitary cells. Using Nomarski differential interference-contrast microscopy, the unique morphologies of discrete subpopulations of cells were characterized. To aid in the initial characterization of different pituitary-cell types, a discontinuous Percoll density-gradient cell-separation technique was developed. This method provided fractions enriched with functional gonadotropin, growth-hormone, and prolactin cells. The morphology of each cell type was initially characterized in enriched fractions of immunofluorescently labelled cells using differential interference-contrast microscopy. The cell type-specific morphologies were then confirmed in live pituitary-cell cultures. Gonadotropin, growth-hormone, and prolactin cells were correctly identified in live mixed cultures in 92, 94, and 100% of the trials, respectively. The ability to directly identify cells in primary cultures allows the physiological study of identified single cells with minimal pretreatment.
Persistent Identifierhttp://hdl.handle.net/10722/84858
ISSN
2015 Impact Factor: 2.948
2015 SCImago Journal Rankings: 1.539

 

DC FieldValueLanguage
dc.contributor.authorVan Goor, Fen_HK
dc.contributor.authorGoldberg, JIen_HK
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorJobin, RMen_HK
dc.contributor.authorChang, JPen_HK
dc.date.accessioned2010-09-06T08:57:56Z-
dc.date.available2010-09-06T08:57:56Z-
dc.date.issued1994en_HK
dc.identifier.citationCell And Tissue Research, 1994, v. 276 n. 2, p. 253-261en_HK
dc.identifier.issn0302-766Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84858-
dc.description.abstractTo better understand neuroendocrine regulation and the intracellular mechanisms mediating pituitary-hormone release, it is necessary to study the physiology of identified single cells. We have developed a system to identify gonadotropin, growth-hormone, and prolactin cells in primary cultures of goldfish pituitary cells. Using Nomarski differential interference-contrast microscopy, the unique morphologies of discrete subpopulations of cells were characterized. To aid in the initial characterization of different pituitary-cell types, a discontinuous Percoll density-gradient cell-separation technique was developed. This method provided fractions enriched with functional gonadotropin, growth-hormone, and prolactin cells. The morphology of each cell type was initially characterized in enriched fractions of immunofluorescently labelled cells using differential interference-contrast microscopy. The cell type-specific morphologies were then confirmed in live pituitary-cell cultures. Gonadotropin, growth-hormone, and prolactin cells were correctly identified in live mixed cultures in 92, 94, and 100% of the trials, respectively. The ability to directly identify cells in primary cultures allows the physiological study of identified single cells with minimal pretreatment.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htmen_HK
dc.relation.ispartofCell and Tissue Researchen_HK
dc.subjectCell identificationen_HK
dc.subjectCell separationen_HK
dc.subjectDifferential interference-contrast (Nomarski) microscopyen_HK
dc.subjectGonadotropin cellsen_HK
dc.subjectGrowth-hormone cellsen_HK
dc.subjectImmunofluorescenceen_HK
dc.subjectProlactin cellsen_HK
dc.subjectTeleostei goldfishen_HK
dc.titleMorphological identification of live gonadotropin, growth-hormone and prolactin cells in goldfish (Carassius auratus) pituitary-cell culturesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0302-766X&volume=276&spage=253&epage=261&date=1994&atitle=Morphological+identification+of+live+gonadotropin,+growth+hormone,+and+prolactin+cells+in+goldfish+(Carassius+auratus)+pituitary+cell+cultures.en_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0028280255en_HK
dc.identifier.hkuros5763en_HK
dc.identifier.volume276en_HK
dc.identifier.issue2en_HK
dc.identifier.spage253en_HK
dc.identifier.epage261en_HK
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridVan Goor, F=35845505200en_HK
dc.identifier.scopusauthoridGoldberg, JI=7403090439en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK
dc.identifier.scopusauthoridJobin, RM=6701719589en_HK
dc.identifier.scopusauthoridChang, JP=7601547649en_HK

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