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Article: Epidermal growth factor (EGF) receptor ligands in the chicken ovary: I. Evidence for heparin-binding EGF-like growth factor (HB-EGF) as a potential oocyte-derived signal to control granulosa cell proliferation and HB-EGF and kit ligand expression

TitleEpidermal growth factor (EGF) receptor ligands in the chicken ovary: I. Evidence for heparin-binding EGF-like growth factor (HB-EGF) as a potential oocyte-derived signal to control granulosa cell proliferation and HB-EGF and kit ligand expression
Authors
Issue Date2007
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2007, v. 148 n. 7, p. 3426-3440 How to Cite?
AbstractThere is increasing evidence that epidermal growth factor (EGF) receptor (EGFR) ligand and Kit ligand (KL) play critical roles in controlling follicular development in mammals. Because little is known about their expressions in the ovary of nonmammalian vertebrate, our study aimed to examine the expression, hormonal regulation, and interaction of HB-EGF and KL in the chicken ovary. Using semiquantitative RT-PCR, we demonstrated that ovarian HB-EGF expression increased dramatically with the posthatching ovarian growth. In line with this finding, HB-EGF was shown to be produced primarily by the growing oocytes and capable of stimulating the proliferation of granulosa cells in prehierarchal (3 mm) and preovulatory follicles (F5 and F1). Although HB-EGF expression is mainly restricted to the oocytes, its expression in cultured granulosa cells could be transiently yet strongly induced by HB-EGF and other EGFR ligands including EGF and TGF-α. And the inducing effect of HB-EGF was completely abolished by AG1478 (10 μM) or PD98059 (100 μM), indicating that the action of HB-EGF is mediated by EGFR and intracellular MAPK/ERK signaling pathway. Unlike mammals, only KL-1, not the other three isoforms identified (KL-2, -3, and -4), was detected to be predominantly expressed in the chicken ovary. Interestingly, KL expression in undifferentiated and differentiated granulosa cells could be transiently down-regulated by HB-EGF, implying an intrafollicular communication between growing oocyte and surrounding granulosa cells through the interplay of EGFR ligand and KL. Collectively, our data suggest that HB-EGF is likely a paracrine signal from the oocyte to regulate granulosa cell proliferation and HB-EGF and KL expression during ovarian follicular development. Copyright © 2007 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/84855
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorWang, CYen_HK
dc.contributor.authorKwok, AHYen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2010-09-06T08:57:54Z-
dc.date.available2010-09-06T08:57:54Z-
dc.date.issued2007en_HK
dc.identifier.citationEndocrinology, 2007, v. 148 n. 7, p. 3426-3440en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84855-
dc.description.abstractThere is increasing evidence that epidermal growth factor (EGF) receptor (EGFR) ligand and Kit ligand (KL) play critical roles in controlling follicular development in mammals. Because little is known about their expressions in the ovary of nonmammalian vertebrate, our study aimed to examine the expression, hormonal regulation, and interaction of HB-EGF and KL in the chicken ovary. Using semiquantitative RT-PCR, we demonstrated that ovarian HB-EGF expression increased dramatically with the posthatching ovarian growth. In line with this finding, HB-EGF was shown to be produced primarily by the growing oocytes and capable of stimulating the proliferation of granulosa cells in prehierarchal (3 mm) and preovulatory follicles (F5 and F1). Although HB-EGF expression is mainly restricted to the oocytes, its expression in cultured granulosa cells could be transiently yet strongly induced by HB-EGF and other EGFR ligands including EGF and TGF-α. And the inducing effect of HB-EGF was completely abolished by AG1478 (10 μM) or PD98059 (100 μM), indicating that the action of HB-EGF is mediated by EGFR and intracellular MAPK/ERK signaling pathway. Unlike mammals, only KL-1, not the other three isoforms identified (KL-2, -3, and -4), was detected to be predominantly expressed in the chicken ovary. Interestingly, KL expression in undifferentiated and differentiated granulosa cells could be transiently down-regulated by HB-EGF, implying an intrafollicular communication between growing oocyte and surrounding granulosa cells through the interplay of EGFR ligand and KL. Collectively, our data suggest that HB-EGF is likely a paracrine signal from the oocyte to regulate granulosa cell proliferation and HB-EGF and KL expression during ovarian follicular development. Copyright © 2007 by The Endocrine Society.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsEndocrinology. Copyright © The Endocrine Society.en_HK
dc.titleEpidermal growth factor (EGF) receptor ligands in the chicken ovary: I. Evidence for heparin-binding EGF-like growth factor (HB-EGF) as a potential oocyte-derived signal to control granulosa cell proliferation and HB-EGF and kit ligand expressionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=148&spage=3426&epage=3440&date=2007&atitle=Epidermal+Growth+Factor+(EGF)+Receptor+Ligands+in+the+Chicken+Ovary:+I.+Evidence+for+Heparin-Binding+EGF-Like+Growth+Factor+(HB-EGF)+as+a+Potential+Oocyte-Derived+Signal+to+Control+Granulosa+Cell+Proliferation+and+HB-EGF+and+Kit+Ligand+Expressionen_HK
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00801en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2006-1383en_HK
dc.identifier.pmid17395697-
dc.identifier.scopuseid_2-s2.0-34347268442en_HK
dc.identifier.hkuros133518en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34347268442&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume148en_HK
dc.identifier.issue7en_HK
dc.identifier.spage3426en_HK
dc.identifier.epage3440en_HK
dc.identifier.isiWOS:000247302300046-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, Y=36062525200en_HK
dc.identifier.scopusauthoridLi, J=27171035300en_HK
dc.identifier.scopusauthoridWang, CY=16065173000en_HK
dc.identifier.scopusauthoridKwok, AHY=27168105100en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK

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