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Article: Isolation and characterization of repetitive DNA sequences from Panax ginseng

TitleIsolation and characterization of repetitive DNA sequences from Panax ginseng
Authors
KeywordsDNA fingerprinting
Panax ginseng
Panax quinquefolius
Repetitive DNA
Retrotransposon
Issue Date2001
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htm
Citation
Molecular Genetics And Genomics, 2001, v. 266 n. 6, p. 951-961 How to Cite?
AbstractRepetitive sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of repetitive DNA sequences provide an insight into the organization and evolution of the genome of interest. We report the isolation and characterization of the major classes of repetitive sequences from the genome of Panax ginseng. The isolation of repetitive DNA from P. ginseng was achieved by the reannealing of chemically hydrolyzed (200 bp1 kb fragments) and heat-denatured genomic DNA to low Cot value. The low Cot fraction was cloned, and fifty-five P. ginseng clones were identified that contained repetitive sequences. Sequence analysis revealed that the fraction includes repetitive telomeric sequences, species-specific satellite sequences, chloroplast DNA fragments and sequences that are homologous to retrotransposons. Two of the retrotransposon-like sequences are homologous to Ty1/copia-type retroelements of Zea mays, and six cloned sequences are homologous to various regions of the del retrotransposon of Lilium henryi. The del retrotransposon-like sequences and several novel repetitive DNA sequences from P. ginseng were used to differentiate P. ginseng from P. quinquefolius, and should be useful for evolutionary studies of these disjunct species.
Persistent Identifierhttp://hdl.handle.net/10722/84850
ISSN
2015 Impact Factor: 2.622
2015 SCImago Journal Rankings: 1.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, Ien_HK
dc.contributor.authorLeung, Fen_HK
dc.date.accessioned2010-09-06T08:57:51Z-
dc.date.available2010-09-06T08:57:51Z-
dc.date.issued2001en_HK
dc.identifier.citationMolecular Genetics And Genomics, 2001, v. 266 n. 6, p. 951-961en_HK
dc.identifier.issn1617-4615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84850-
dc.description.abstractRepetitive sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of repetitive DNA sequences provide an insight into the organization and evolution of the genome of interest. We report the isolation and characterization of the major classes of repetitive sequences from the genome of Panax ginseng. The isolation of repetitive DNA from P. ginseng was achieved by the reannealing of chemically hydrolyzed (200 bp1 kb fragments) and heat-denatured genomic DNA to low Cot value. The low Cot fraction was cloned, and fifty-five P. ginseng clones were identified that contained repetitive sequences. Sequence analysis revealed that the fraction includes repetitive telomeric sequences, species-specific satellite sequences, chloroplast DNA fragments and sequences that are homologous to retrotransposons. Two of the retrotransposon-like sequences are homologous to Ty1/copia-type retroelements of Zea mays, and six cloned sequences are homologous to various regions of the del retrotransposon of Lilium henryi. The del retrotransposon-like sequences and several novel repetitive DNA sequences from P. ginseng were used to differentiate P. ginseng from P. quinquefolius, and should be useful for evolutionary studies of these disjunct species.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htmen_HK
dc.relation.ispartofMolecular Genetics and Genomicsen_HK
dc.subjectDNA fingerprintingen_HK
dc.subjectPanax ginsengen_HK
dc.subjectPanax quinquefoliusen_HK
dc.subjectRepetitive DNAen_HK
dc.subjectRetrotransposonen_HK
dc.titleIsolation and characterization of repetitive DNA sequences from Panax ginsengen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0026-8925&volume=266&spage=951&epage=961&date=2002&atitle=Isolation+and+characterization+of+repetitive+DNA+sequences+from+Panax+ginsengen_HK
dc.identifier.emailLeung, F: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, F=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00438-001-0617-6en_HK
dc.identifier.pmid11862489-
dc.identifier.scopuseid_2-s2.0-0035718473en_HK
dc.identifier.hkuros65867en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035718473&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume266en_HK
dc.identifier.issue6en_HK
dc.identifier.spage951en_HK
dc.identifier.epage961en_HK
dc.identifier.isiWOS:000174621700006-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridHo, I=15850752500en_HK
dc.identifier.scopusauthoridLeung, F=7103078633en_HK

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