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Article: Molecular cloning of a teleost growth hormone receptor and its functional interaction with human growth hormone

TitleMolecular cloning of a teleost growth hormone receptor and its functional interaction with human growth hormone
Authors
KeywordsComplementary DNA cloning
Functional expression
Goldfish
Growth hormone receptor
Issue Date2001
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
Citation
Gene, 2001, v. 270 n. 1-2, p. 121-129 How to Cite?
AbstractThis paper reports the first full-length cDNA sequence of a growth hormone receptor (GHR) from a teleost fish and its functional expression in cultured eukaryotic cells. The cDNA sequence, from Carassius auratus (goldfish), encodes a protein of 602 amino acids (aa) akin in architecture to the GHRs of other species. Despite the presence of motifs characteristic of GHR, the overall homology between the goldfish GHR and other GHRs is very low (≈ 40% aa identity). CHO cells transfected with this receptor cDNA can be stimulated to proliferate by human growth hormone (hGH). In addition, the transfected cells can transactivate a co-expressed mammalian serine protease inhibitor (Spi) 2.1 promoter upon stimulation by hGH, indicating the successful interaction of the fish receptor with the mammalian ligand to evoke the down-stream post-receptor events. Tissue distribution studies indicated that the receptor is mostly expressed in the liver and hypothalamus of goldfish. A single mRNA transcript of a size of about 4 kb was found in the goldfish liver. © 2001 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/84843
ISSN
2015 Impact Factor: 2.319
2015 SCImago Journal Rankings: 1.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, LTOen_HK
dc.contributor.authorNong, Gen_HK
dc.contributor.authorChan, YHen_HK
dc.contributor.authorTse, DLYen_HK
dc.contributor.authorCheng, CHKen_HK
dc.date.accessioned2010-09-06T08:57:46Z-
dc.date.available2010-09-06T08:57:46Z-
dc.date.issued2001en_HK
dc.identifier.citationGene, 2001, v. 270 n. 1-2, p. 121-129en_HK
dc.identifier.issn0378-1119en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84843-
dc.description.abstractThis paper reports the first full-length cDNA sequence of a growth hormone receptor (GHR) from a teleost fish and its functional expression in cultured eukaryotic cells. The cDNA sequence, from Carassius auratus (goldfish), encodes a protein of 602 amino acids (aa) akin in architecture to the GHRs of other species. Despite the presence of motifs characteristic of GHR, the overall homology between the goldfish GHR and other GHRs is very low (≈ 40% aa identity). CHO cells transfected with this receptor cDNA can be stimulated to proliferate by human growth hormone (hGH). In addition, the transfected cells can transactivate a co-expressed mammalian serine protease inhibitor (Spi) 2.1 promoter upon stimulation by hGH, indicating the successful interaction of the fish receptor with the mammalian ligand to evoke the down-stream post-receptor events. Tissue distribution studies indicated that the receptor is mostly expressed in the liver and hypothalamus of goldfish. A single mRNA transcript of a size of about 4 kb was found in the goldfish liver. © 2001 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneen_HK
dc.relation.ispartofGeneen_HK
dc.rightsGene. Copyright © Elsevier BV.en_HK
dc.subjectComplementary DNA cloningen_HK
dc.subjectFunctional expressionen_HK
dc.subjectGoldfishen_HK
dc.subjectGrowth hormone receptoren_HK
dc.titleMolecular cloning of a teleost growth hormone receptor and its functional interaction with human growth hormoneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-1119&volume=270&spage=121&epage=129&date=2001&atitle=Molecular+cloning+of+a+teleost+growth+hormone+receptor+and+its+functional++interaction+with+human+growth+hormoneen_HK
dc.identifier.emailLee, LTO: ltolee2@hkucc.hku.hken_HK
dc.identifier.authorityLee, LTO=rp00727en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0378-1119(01)00488-7en_HK
dc.identifier.pmid11404009-
dc.identifier.scopuseid_2-s2.0-0035972807en_HK
dc.identifier.hkuros105336en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035972807&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume270en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage121en_HK
dc.identifier.epage129en_HK
dc.identifier.isiWOS:000169549000012-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLee, LTO=8367269000en_HK
dc.identifier.scopusauthoridNong, G=15744371700en_HK
dc.identifier.scopusauthoridChan, YH=7403676269en_HK
dc.identifier.scopusauthoridTse, DLY=7101916515en_HK
dc.identifier.scopusauthoridCheng, CHK=7404798014en_HK

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