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Article: Expression of goldfish growth hormone I gene using baculovirus expression system

TitleExpression of goldfish growth hormone I gene using baculovirus expression system
利用桿狀病毒表達系統表達金魚生長激素I基因
Authors
KeywordsGoldfish growth hormone
Baculovirus
Expression
Secretion
Issue Date1997
Publisher科學出版社.
Citation
生物工程學報, 1997, v. 13 n. 2, p. 149-153 How to Cite?
Chinese Journal of Biotechnology, 1997, v. 13 n. 2, p. 149-153 How to Cite?
AbstractUsing pSXIVVI +X3 without an initiation codon as an expressing vector, an occluded recombinant Trichoplusia ni nuclear polyhedrosis virus carrying the cDNA encoding goldfish growth hormone I (gf GHI) under the control of the Syn XIV promoter, has been constructed. Immunoblot analysis showed that the molecular weight of the expressed protein was 22 5kDa as expected, revealed that the virus mediated gfGHI was synthesized in the Sf cells, larvae and secreted into the supernatant and hemolymph.
Persistent Identifierhttp://hdl.handle.net/10722/84840
ISSN
2015 SCImago Journal Rankings: 0.140

 

DC FieldValueLanguage
dc.contributor.authorLin, GYen_HK
dc.contributor.authorWang, XZen_HK
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorLong, QXen_HK
dc.contributor.authorPang, Yen_HK
dc.contributor.authorYu, KLen_HK
dc.date.accessioned2010-09-06T08:57:44Z-
dc.date.available2010-09-06T08:57:44Z-
dc.date.issued1997en_HK
dc.identifier.citation生物工程學報, 1997, v. 13 n. 2, p. 149-153en_HK
dc.identifier.citationChinese Journal of Biotechnology, 1997, v. 13 n. 2, p. 149-153-
dc.identifier.issn1000-3061en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84840-
dc.description.abstractUsing pSXIVVI +X3 without an initiation codon as an expressing vector, an occluded recombinant Trichoplusia ni nuclear polyhedrosis virus carrying the cDNA encoding goldfish growth hormone I (gf GHI) under the control of the Syn XIV promoter, has been constructed. Immunoblot analysis showed that the molecular weight of the expressed protein was 22 5kDa as expected, revealed that the virus mediated gfGHI was synthesized in the Sf cells, larvae and secreted into the supernatant and hemolymph.-
dc.languagechien_HK
dc.publisher科學出版社.en_HK
dc.relation.ispartof生物工程學報en_HK
dc.relation.ispartofChinese Journal of Biotechnology-
dc.subjectGoldfish growth hormone-
dc.subjectBaculovirus-
dc.subjectExpression-
dc.subjectSecretion-
dc.titleExpression of goldfish growth hormone I gene using baculovirus expression systemen_HK
dc.title利用桿狀病毒表達系統表達金魚生長激素I基因-
dc.typeArticleen_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.emailYu, KL: yukeili@hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.identifier.hkuros26574en_HK
dc.identifier.volume13-
dc.identifier.issue2-
dc.identifier.spage149-
dc.identifier.epage153-
dc.publisher.placeChina-

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