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Article: Expression and transcriptional regulation of the GnRH receptor gene in human neuronal cells

TitleExpression and transcriptional regulation of the GnRH receptor gene in human neuronal cells
Authors
KeywordsGnRH receptor
PKC
Staurosporine
TE-671
Transcriptional regulation
Issue Date2005
PublisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/
Citation
Molecular Human Reproduction, 2005, v. 11 n. 11, p. 837-842 How to Cite?
AbstractGnRH, acts via the GnRH receptor (GnRHR), plays a pivotal role in human reproduction by stimulating the synthesis and secretion of gonadotropins from pituitary gonadotropes. Studies have also suggested that it has other extra-pituitary functions. To date, the transcriptional regulation of human GnRHR gene in the brain remains largely unknown. Recently, the human cerebellar medulloblastoma cell line TE-671 is found to express GnRH. We report here for the first time that GnRHR is also expressed in this neuronal cell line. Treatment with GnRHR agonist stimulated the phosphorylation of both ERK1/2 and JNK in the cells. Moreover, transient transfection of various human GnRHR promoter-luciferase constructs into the cells identified an upstream promoter region located between -2197 and -1018. Important cis-acting regulatory elements were found at -1300/ -1018 and -2197/- 1900, as deletion of either region caused a dramatic decrease in the promoter activity. An upstream GnRHR promoter element was identified to be important for basal transcription in the human neuronal TE-671 cells, in contrast to the previous finding that a downstream promoter is responsible for the gonadotrope-specific expression. Furthermore, we showed that antide (GnRHR antagonist) significantly stimulated the GnRHR promoter activity and inhibition of protein kinase C (PKC) pathway by staurosporine could also up-regulate the promoter activity in dose- and time-dependent manners. Taken together, these data suggest that activation of the GnRHR by interacting with GnRH may transcriptionally down-regulate itself via the PKC pathway in human neuronal cells. © The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/84839
ISSN
2015 Impact Factor: 3.943
2015 SCImago Journal Rankings: 1.611
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, CMen_HK
dc.contributor.authorAn, BSen_HK
dc.contributor.authorCheng, CKen_HK
dc.contributor.authorChow, BKCen_HK
dc.contributor.authorLeung, PCKen_HK
dc.date.accessioned2010-09-06T08:57:43Z-
dc.date.available2010-09-06T08:57:43Z-
dc.date.issued2005en_HK
dc.identifier.citationMolecular Human Reproduction, 2005, v. 11 n. 11, p. 837-842en_HK
dc.identifier.issn1360-9947en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84839-
dc.description.abstractGnRH, acts via the GnRH receptor (GnRHR), plays a pivotal role in human reproduction by stimulating the synthesis and secretion of gonadotropins from pituitary gonadotropes. Studies have also suggested that it has other extra-pituitary functions. To date, the transcriptional regulation of human GnRHR gene in the brain remains largely unknown. Recently, the human cerebellar medulloblastoma cell line TE-671 is found to express GnRH. We report here for the first time that GnRHR is also expressed in this neuronal cell line. Treatment with GnRHR agonist stimulated the phosphorylation of both ERK1/2 and JNK in the cells. Moreover, transient transfection of various human GnRHR promoter-luciferase constructs into the cells identified an upstream promoter region located between -2197 and -1018. Important cis-acting regulatory elements were found at -1300/ -1018 and -2197/- 1900, as deletion of either region caused a dramatic decrease in the promoter activity. An upstream GnRHR promoter element was identified to be important for basal transcription in the human neuronal TE-671 cells, in contrast to the previous finding that a downstream promoter is responsible for the gonadotrope-specific expression. Furthermore, we showed that antide (GnRHR antagonist) significantly stimulated the GnRHR promoter activity and inhibition of protein kinase C (PKC) pathway by staurosporine could also up-regulate the promoter activity in dose- and time-dependent manners. Taken together, these data suggest that activation of the GnRHR by interacting with GnRH may transcriptionally down-regulate itself via the PKC pathway in human neuronal cells. © The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/en_HK
dc.relation.ispartofMolecular Human Reproductionen_HK
dc.rightsMolecular Human Reproduction. Copyright © Oxford University Press.en_HK
dc.subjectGnRH receptoren_HK
dc.subjectPKCen_HK
dc.subjectStaurosporineen_HK
dc.subjectTE-671en_HK
dc.subjectTranscriptional regulationen_HK
dc.titleExpression and transcriptional regulation of the GnRH receptor gene in human neuronal cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1360-9947&volume=11&issue=11&spage=837&epage=842&date=2005&atitle=Expression+and+transcriptional+regulation+of+the+GnRH+receptor+gene+in+human+neuronal+cells.en_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/molehr/gah241en_HK
dc.identifier.pmid16364974-
dc.identifier.scopuseid_2-s2.0-32144453059en_HK
dc.identifier.hkuros125068en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-32144453059&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue11en_HK
dc.identifier.spage837en_HK
dc.identifier.epage842en_HK
dc.identifier.isiWOS:000235278700009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYeung, CM=7201354151en_HK
dc.identifier.scopusauthoridAn, BS=7007154153en_HK
dc.identifier.scopusauthoridCheng, CK=7404797040en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK
dc.identifier.scopusauthoridLeung, PCK=55419381000en_HK
dc.identifier.citeulike495519-

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