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Article: Identification of Rice Hybrid Seed Using Allozyme Markers

TitleIdentification of Rice Hybrid Seed Using Allozyme Markers
等位酶標記快速鑒定雜交水稻種子初步研究
Authors
KeywordsHybrid rice
Allozyme marker
Identification of hybrid seed
Starch gel electrophoresis
Issue Date1997
Publisher高技朮通訊雜誌社.
Citation
高技術通訊, 1997, v. 7 n. 8, p. 41-45 How to Cite?
High Technology Letters, 1997, v. 7 n. 8, p. 41-45 How to Cite?
AbstractIn this study, 32 loci encoding 21 enzyme systems for Allozyme markers that can be used to identify each of the nine hybrids studied were obtained. Of the 32 isozyme loci surveyed, ten were polymorphic with a total of 25 alleies present in the nine hybrids, and five of the polymorphic loci could be used as markers for hybrid identification, as the parental lines of these hybrids were homozygous for different alleies at one or more of the loci. Using these allozyme markers, the purity of hybrid seeds were investigated by random sampling of 100 seedlings of the hybrid line and 10 seedlings each from the parents. The purity of the hybrid seed ranged from 60~100% in the nine hybrid lines was tested. By comparing the hybrid genotype with their respective parents, the source of impurity can be identified. In all the cases, comparing parental and hybrid seeds, the non-hybrid genotypes were found to be identical to the maternal parent at the marker loci , indicating that the primary source of contamination in the hybrid seed is self-fertilization in the maternal parent.
Persistent Identifierhttp://hdl.handle.net/10722/84838
ISSN
2015 SCImago Journal Rankings: 0.107

 

DC FieldValueLanguage
dc.contributor.authorFang, Xen_HK
dc.contributor.authorMou, Ten_HK
dc.contributor.authorLu, Xen_HK
dc.contributor.authorSun, Men_HK
dc.date.accessioned2010-09-06T08:57:42Z-
dc.date.available2010-09-06T08:57:42Z-
dc.date.issued1997en_HK
dc.identifier.citation高技術通訊, 1997, v. 7 n. 8, p. 41-45en_HK
dc.identifier.citationHigh Technology Letters, 1997, v. 7 n. 8, p. 41-45-
dc.identifier.issn1002-0470en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84838-
dc.description.abstractIn this study, 32 loci encoding 21 enzyme systems for Allozyme markers that can be used to identify each of the nine hybrids studied were obtained. Of the 32 isozyme loci surveyed, ten were polymorphic with a total of 25 alleies present in the nine hybrids, and five of the polymorphic loci could be used as markers for hybrid identification, as the parental lines of these hybrids were homozygous for different alleies at one or more of the loci. Using these allozyme markers, the purity of hybrid seeds were investigated by random sampling of 100 seedlings of the hybrid line and 10 seedlings each from the parents. The purity of the hybrid seed ranged from 60~100% in the nine hybrid lines was tested. By comparing the hybrid genotype with their respective parents, the source of impurity can be identified. In all the cases, comparing parental and hybrid seeds, the non-hybrid genotypes were found to be identical to the maternal parent at the marker loci , indicating that the primary source of contamination in the hybrid seed is self-fertilization in the maternal parent.-
dc.languagechien_HK
dc.publisher高技朮通訊雜誌社.en_HK
dc.relation.ispartof高技術通訊en_HK
dc.relation.ispartofHigh Technology Letters-
dc.subjectHybrid rice-
dc.subjectAllozyme marker-
dc.subjectIdentification of hybrid seed-
dc.subjectStarch gel electrophoresis-
dc.titleIdentification of Rice Hybrid Seed Using Allozyme Markersen_HK
dc.title等位酶標記快速鑒定雜交水稻種子初步研究-
dc.typeArticleen_HK
dc.identifier.emailSun, M: meisun@hkucc.hku.hken_HK
dc.identifier.authoritySun, M=rp00779en_HK
dc.identifier.hkuros22577en_HK
dc.identifier.volume7-
dc.identifier.issue8-
dc.identifier.spage41-
dc.identifier.epage45-
dc.publisher.placeChina-

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