File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Functional and comparative analysis of globin loci in pufferfish and humans
  • Basic View
  • Metadata View
  • XML View
TitleFunctional and comparative analysis of globin loci in pufferfish and humans
 
AuthorsGillemans, N
McMorrow, T
Tewari, R
Wai, AWK
Burgtorf, C
Drabek, D
Ventress, N
Langeveld, A
Higgs, D
TanUn, K
Grosveld, F
Philipsen, S1
 
Issue Date2003
 
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
 
CitationBlood, 2003, v. 101 n. 7, p. 2842-2849 [How to Cite?]
DOI: http://dx.doi.org/10.1182/blood-2002-09-2850
 
AbstractTo further our understanding of the regulation of vertebrate globin loci, we have isolated cosmids containing α- and β-globin genes from the pufferfish Fugu rubripes. By DNA fluorescence in situ hybridization (FISH) analysis, we show that Fugu contains 2 distinct hemoglobin loci situated on separate chromosomes. One locus contains only α-globin genes (α-locus), whereas the other also contains a β-globin gene (αβ-locus). This is the first poikilothermic species analyzed in which the physical linkage of the α- and β-globin genes has been uncoupled, supporting a model in which the separation of the α- and β-globin loci has occurred through duplication of a locus containing both types of genes. Surveys for transcription factor binding sites and DNaseI hypersensitive site mapping of the Fugu αβ-locus suggest that a strong distal locus control region regulating the activity of the globin genes, as found in mammalian β-globin clusters, may not be present in the Fugu αβ-locus. Searching the human and mouse genome databases with the genes surrounding the pufferfish hemoglobin loci reveals that homologues of some of these genes are proximal to cytoglobin, a recently described novel member of the globin family. This provides evidence that duplication of the globin loci has occurred several times during evolution, resulting in the 5 human globin loci known to date, each encoding proteins with specific functions in specific cell types. © 2003 by The American Society of Hematology.
 
ISSN0006-4971
2012 Impact Factor: 9.06
2012 SCImago Journal Rankings: 4.553
 
DOIhttp://dx.doi.org/10.1182/blood-2002-09-2850
 
ISI Accession Number IDWOS:000181823600064
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorGillemans, N
 
dc.contributor.authorMcMorrow, T
 
dc.contributor.authorTewari, R
 
dc.contributor.authorWai, AWK
 
dc.contributor.authorBurgtorf, C
 
dc.contributor.authorDrabek, D
 
dc.contributor.authorVentress, N
 
dc.contributor.authorLangeveld, A
 
dc.contributor.authorHiggs, D
 
dc.contributor.authorTanUn, K
 
dc.contributor.authorGrosveld, F
 
dc.contributor.authorPhilipsen, S
 
dc.date.accessioned2010-09-06T08:57:40Z
 
dc.date.available2010-09-06T08:57:40Z
 
dc.date.issued2003
 
dc.description.abstractTo further our understanding of the regulation of vertebrate globin loci, we have isolated cosmids containing α- and β-globin genes from the pufferfish Fugu rubripes. By DNA fluorescence in situ hybridization (FISH) analysis, we show that Fugu contains 2 distinct hemoglobin loci situated on separate chromosomes. One locus contains only α-globin genes (α-locus), whereas the other also contains a β-globin gene (αβ-locus). This is the first poikilothermic species analyzed in which the physical linkage of the α- and β-globin genes has been uncoupled, supporting a model in which the separation of the α- and β-globin loci has occurred through duplication of a locus containing both types of genes. Surveys for transcription factor binding sites and DNaseI hypersensitive site mapping of the Fugu αβ-locus suggest that a strong distal locus control region regulating the activity of the globin genes, as found in mammalian β-globin clusters, may not be present in the Fugu αβ-locus. Searching the human and mouse genome databases with the genes surrounding the pufferfish hemoglobin loci reveals that homologues of some of these genes are proximal to cytoglobin, a recently described novel member of the globin family. This provides evidence that duplication of the globin loci has occurred several times during evolution, resulting in the 5 human globin loci known to date, each encoding proteins with specific functions in specific cell types. © 2003 by The American Society of Hematology.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBlood, 2003, v. 101 n. 7, p. 2842-2849 [How to Cite?]
DOI: http://dx.doi.org/10.1182/blood-2002-09-2850
 
dc.identifier.doihttp://dx.doi.org/10.1182/blood-2002-09-2850
 
dc.identifier.epage2849
 
dc.identifier.hkuros76084
 
dc.identifier.isiWOS:000181823600064
 
dc.identifier.issn0006-4971
2012 Impact Factor: 9.06
2012 SCImago Journal Rankings: 4.553
 
dc.identifier.issue7
 
dc.identifier.openurl
 
dc.identifier.pmid12517812
 
dc.identifier.scopuseid_2-s2.0-0038107364
 
dc.identifier.spage2842
 
dc.identifier.urihttp://hdl.handle.net/10722/84835
 
dc.identifier.volume101
 
dc.languageeng
 
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBlood
 
dc.relation.referencesReferences in Scopus
 
dc.titleFunctional and comparative analysis of globin loci in pufferfish and humans
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Gillemans, N</contributor.author>
<contributor.author>McMorrow, T</contributor.author>
<contributor.author>Tewari, R</contributor.author>
<contributor.author>Wai, AWK</contributor.author>
<contributor.author>Burgtorf, C</contributor.author>
<contributor.author>Drabek, D</contributor.author>
<contributor.author>Ventress, N</contributor.author>
<contributor.author>Langeveld, A</contributor.author>
<contributor.author>Higgs, D</contributor.author>
<contributor.author>TanUn, K</contributor.author>
<contributor.author>Grosveld, F</contributor.author>
<contributor.author>Philipsen, S</contributor.author>
<date.accessioned>2010-09-06T08:57:40Z</date.accessioned>
<date.available>2010-09-06T08:57:40Z</date.available>
<date.issued>2003</date.issued>
<identifier.citation>Blood, 2003, v. 101 n. 7, p. 2842-2849</identifier.citation>
<identifier.issn>0006-4971</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/84835</identifier.uri>
<description.abstract>To further our understanding of the regulation of vertebrate globin loci, we have isolated cosmids containing &#945;- and &#946;-globin genes from the pufferfish Fugu rubripes. By DNA fluorescence in situ hybridization (FISH) analysis, we show that Fugu contains 2 distinct hemoglobin loci situated on separate chromosomes. One locus contains only &#945;-globin genes (&#945;-locus), whereas the other also contains a &#946;-globin gene (&#945;&#946;-locus). This is the first poikilothermic species analyzed in which the physical linkage of the &#945;- and &#946;-globin genes has been uncoupled, supporting a model in which the separation of the &#945;- and &#946;-globin loci has occurred through duplication of a locus containing both types of genes. Surveys for transcription factor binding sites and DNaseI hypersensitive site mapping of the Fugu &#945;&#946;-locus suggest that a strong distal locus control region regulating the activity of the globin genes, as found in mammalian &#946;-globin clusters, may not be present in the Fugu &#945;&#946;-locus. Searching the human and mouse genome databases with the genes surrounding the pufferfish hemoglobin loci reveals that homologues of some of these genes are proximal to cytoglobin, a recently described novel member of the globin family. This provides evidence that duplication of the globin loci has occurred several times during evolution, resulting in the 5 human globin loci known to date, each encoding proteins with specific functions in specific cell types. &#169; 2003 by The American Society of Hematology.</description.abstract>
<language>eng</language>
<publisher>American Society of Hematology. The Journal&apos;s web site is located at http://bloodjournal.hematologylibrary.org/</publisher>
<relation.ispartof>Blood</relation.ispartof>
<title>Functional and comparative analysis of globin loci in pufferfish and humans</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=0006-4971&amp;volume=101&amp;spage=2842&amp;epage=2849&amp;date=2003&amp;atitle=Functional+and+comparative+analysis+of+globin+loci+in+pufferfish+and+humans</identifier.openurl>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1182/blood-2002-09-2850</identifier.doi>
<identifier.pmid>12517812</identifier.pmid>
<identifier.scopus>eid_2-s2.0-0038107364</identifier.scopus>
<identifier.hkuros>76084</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-0038107364&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>101</identifier.volume>
<identifier.issue>7</identifier.issue>
<identifier.spage>2842</identifier.spage>
<identifier.epage>2849</identifier.epage>
<identifier.isi>WOS:000181823600064</identifier.isi>
<publisher.place>United States</publisher.place>
</item>
Author Affiliations
  1. Erasmus University Rotterdam