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Article: Functional and comparative analysis of globin loci in pufferfish and humans

TitleFunctional and comparative analysis of globin loci in pufferfish and humans
Authors
Issue Date2003
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 2003, v. 101 n. 7, p. 2842-2849 How to Cite?
Abstract
To further our understanding of the regulation of vertebrate globin loci, we have isolated cosmids containing α- and β-globin genes from the pufferfish Fugu rubripes. By DNA fluorescence in situ hybridization (FISH) analysis, we show that Fugu contains 2 distinct hemoglobin loci situated on separate chromosomes. One locus contains only α-globin genes (α-locus), whereas the other also contains a β-globin gene (αβ-locus). This is the first poikilothermic species analyzed in which the physical linkage of the α- and β-globin genes has been uncoupled, supporting a model in which the separation of the α- and β-globin loci has occurred through duplication of a locus containing both types of genes. Surveys for transcription factor binding sites and DNaseI hypersensitive site mapping of the Fugu αβ-locus suggest that a strong distal locus control region regulating the activity of the globin genes, as found in mammalian β-globin clusters, may not be present in the Fugu αβ-locus. Searching the human and mouse genome databases with the genes surrounding the pufferfish hemoglobin loci reveals that homologues of some of these genes are proximal to cytoglobin, a recently described novel member of the globin family. This provides evidence that duplication of the globin loci has occurred several times during evolution, resulting in the 5 human globin loci known to date, each encoding proteins with specific functions in specific cell types. © 2003 by The American Society of Hematology.
Persistent Identifierhttp://hdl.handle.net/10722/84835
ISSN
2013 Impact Factor: 9.775
ISI Accession Number ID
References

 

Author Affiliations
  1. Erasmus University Rotterdam
DC FieldValueLanguage
dc.contributor.authorGillemans, Nen_HK
dc.contributor.authorMcMorrow, Ten_HK
dc.contributor.authorTewari, Ren_HK
dc.contributor.authorWai, AWKen_HK
dc.contributor.authorBurgtorf, Cen_HK
dc.contributor.authorDrabek, Den_HK
dc.contributor.authorVentress, Nen_HK
dc.contributor.authorLangeveld, Aen_HK
dc.contributor.authorHiggs, Den_HK
dc.contributor.authorTanUn, Ken_HK
dc.contributor.authorGrosveld, Fen_HK
dc.contributor.authorPhilipsen, Sen_HK
dc.date.accessioned2010-09-06T08:57:40Z-
dc.date.available2010-09-06T08:57:40Z-
dc.date.issued2003en_HK
dc.identifier.citationBlood, 2003, v. 101 n. 7, p. 2842-2849en_HK
dc.identifier.issn0006-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84835-
dc.description.abstractTo further our understanding of the regulation of vertebrate globin loci, we have isolated cosmids containing α- and β-globin genes from the pufferfish Fugu rubripes. By DNA fluorescence in situ hybridization (FISH) analysis, we show that Fugu contains 2 distinct hemoglobin loci situated on separate chromosomes. One locus contains only α-globin genes (α-locus), whereas the other also contains a β-globin gene (αβ-locus). This is the first poikilothermic species analyzed in which the physical linkage of the α- and β-globin genes has been uncoupled, supporting a model in which the separation of the α- and β-globin loci has occurred through duplication of a locus containing both types of genes. Surveys for transcription factor binding sites and DNaseI hypersensitive site mapping of the Fugu αβ-locus suggest that a strong distal locus control region regulating the activity of the globin genes, as found in mammalian β-globin clusters, may not be present in the Fugu αβ-locus. Searching the human and mouse genome databases with the genes surrounding the pufferfish hemoglobin loci reveals that homologues of some of these genes are proximal to cytoglobin, a recently described novel member of the globin family. This provides evidence that duplication of the globin loci has occurred several times during evolution, resulting in the 5 human globin loci known to date, each encoding proteins with specific functions in specific cell types. © 2003 by The American Society of Hematology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_HK
dc.relation.ispartofBlooden_HK
dc.titleFunctional and comparative analysis of globin loci in pufferfish and humansen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-4971&volume=101&spage=2842&epage=2849&date=2003&atitle=Functional+and+comparative+analysis+of+globin+loci+in+pufferfish+and+humansen_HK
dc.identifier.emailTanUn, K: kctanun@hkucc.hku.hken_HK
dc.identifier.authorityTanUn, K=rp00787en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1182/blood-2002-09-2850en_HK
dc.identifier.pmid12517812-
dc.identifier.scopuseid_2-s2.0-0038107364en_HK
dc.identifier.hkuros76084en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038107364&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume101en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2842en_HK
dc.identifier.epage2849en_HK
dc.identifier.isiWOS:000181823600064-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGillemans, N=6602829906en_HK
dc.identifier.scopusauthoridMcMorrow, T=6603304189en_HK
dc.identifier.scopusauthoridTewari, R=8343732400en_HK
dc.identifier.scopusauthoridWai, AWK=6603455255en_HK
dc.identifier.scopusauthoridBurgtorf, C=6603629437en_HK
dc.identifier.scopusauthoridDrabek, D=6602828815en_HK
dc.identifier.scopusauthoridVentress, N=6507179217en_HK
dc.identifier.scopusauthoridLangeveld, A=6602683281en_HK
dc.identifier.scopusauthoridHiggs, D=7102307960en_HK
dc.identifier.scopusauthoridTanUn, K=6602914262en_HK
dc.identifier.scopusauthoridGrosveld, F=7101858903en_HK
dc.identifier.scopusauthoridPhilipsen, S=7003745052en_HK

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