Article: Functional and comparative analysis of globin loci in pufferfish and humans

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TitleFunctional and comparative analysis of globin loci in pufferfish and humans
AuthorsGillemans, N
McMorrow, T
Tewari, R
Wai, AWK
Burgtorf, C
Drabek, D
Ventress, N
Langeveld, A
Higgs, D
TanUn, K
Grosveld, F
Philipsen, S1
Issue Date2003
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
CitationBlood, 2003, v. 101 n. 7, p. 2842-2849 [How to Cite?]
DOI: http://dx.doi.org/10.1182/blood-2002-09-2850
AbstractTo further our understanding of the regulation of vertebrate globin loci, we have isolated cosmids containing α- and β-globin genes from the pufferfish Fugu rubripes. By DNA fluorescence in situ hybridization (FISH) analysis, we show that Fugu contains 2 distinct hemoglobin loci situated on separate chromosomes. One locus contains only α-globin genes (α-locus), whereas the other also contains a β-globin gene (αβ-locus). This is the first poikilothermic species analyzed in which the physical linkage of the α- and β-globin genes has been uncoupled, supporting a model in which the separation of the α- and β-globin loci has occurred through duplication of a locus containing both types of genes. Surveys for transcription factor binding sites and DNaseI hypersensitive site mapping of the Fugu αβ-locus suggest that a strong distal locus control region regulating the activity of the globin genes, as found in mammalian β-globin clusters, may not be present in the Fugu αβ-locus. Searching the human and mouse genome databases with the genes surrounding the pufferfish hemoglobin loci reveals that homologues of some of these genes are proximal to cytoglobin, a recently described novel member of the globin family. This provides evidence that duplication of the globin loci has occurred several times during evolution, resulting in the 5 human globin loci known to date, each encoding proteins with specific functions in specific cell types. © 2003 by The American Society of Hematology.
ISSN0006-4971
2011 Impact Factor: 9.898
2011 SCImago Journal Rankings: 1.698
DOIhttp://dx.doi.org/10.1182/blood-2002-09-2850
ISI Accession Number IDWOS:000181823600064
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorGillemans, N
dc.contributor.authorMcMorrow, T
dc.contributor.authorTewari, R
dc.contributor.authorWai, AWK
dc.contributor.authorBurgtorf, C
dc.contributor.authorDrabek, D
dc.contributor.authorVentress, N
dc.contributor.authorLangeveld, A
dc.contributor.authorHiggs, D
dc.contributor.authorTanUn, K
dc.contributor.authorGrosveld, F
dc.contributor.authorPhilipsen, S
dc.date.accessioned2010-09-06T08:57:40Z
dc.date.available2010-09-06T08:57:40Z
dc.date.issued2003
dc.description.abstractTo further our understanding of the regulation of vertebrate globin loci, we have isolated cosmids containing α- and β-globin genes from the pufferfish Fugu rubripes. By DNA fluorescence in situ hybridization (FISH) analysis, we show that Fugu contains 2 distinct hemoglobin loci situated on separate chromosomes. One locus contains only α-globin genes (α-locus), whereas the other also contains a β-globin gene (αβ-locus). This is the first poikilothermic species analyzed in which the physical linkage of the α- and β-globin genes has been uncoupled, supporting a model in which the separation of the α- and β-globin loci has occurred through duplication of a locus containing both types of genes. Surveys for transcription factor binding sites and DNaseI hypersensitive site mapping of the Fugu αβ-locus suggest that a strong distal locus control region regulating the activity of the globin genes, as found in mammalian β-globin clusters, may not be present in the Fugu αβ-locus. Searching the human and mouse genome databases with the genes surrounding the pufferfish hemoglobin loci reveals that homologues of some of these genes are proximal to cytoglobin, a recently described novel member of the globin family. This provides evidence that duplication of the globin loci has occurred several times during evolution, resulting in the 5 human globin loci known to date, each encoding proteins with specific functions in specific cell types. © 2003 by The American Society of Hematology.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationBlood, 2003, v. 101 n. 7, p. 2842-2849 [How to Cite?]
DOI: http://dx.doi.org/10.1182/blood-2002-09-2850
dc.identifier.doihttp://dx.doi.org/10.1182/blood-2002-09-2850
dc.identifier.epage2849
dc.identifier.hkuros76084
dc.identifier.isiWOS:000181823600064
dc.identifier.issn0006-4971
2011 Impact Factor: 9.898
2011 SCImago Journal Rankings: 1.698
dc.identifier.issue7
dc.identifier.openurl
dc.identifier.pmid12517812
dc.identifier.scopuseid_2-s2.0-0038107364
dc.identifier.spage2842
dc.identifier.urihttp://hdl.handle.net/10722/84835
dc.identifier.volume101
dc.languageeng
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
dc.publisher.placeUnited States
dc.relation.ispartofBlood
dc.relation.referencesReferences in Scopus
dc.titleFunctional and comparative analysis of globin loci in pufferfish and humans
dc.typeArticle
Author Affiliations
  1. Erasmus University Rotterdam