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Article: Cloning of an Alu-like Sequence from Rat Low-Cot DNA Library and Its Application in the Analysis of Genomic DNA Mutation

TitleCloning of an Alu-like Sequence from Rat Low-Cot DNA Library and Its Application in the Analysis of Genomic DNA Mutation
大鼠類Alu重復序列的克隆及其在染色體DNA突變分析中的應用
Authors
KeywordsRat genome
Low Cot DNA
Alu like sequence
(AGGG) 7 probe
Issue Date2000
Publisher科學出版社. The Journal's web site is located at http://www.cibj.com/
Citation
應用與環境生物學報, 2000, v. 6 n. 1, p. 48-51 How to Cite?
Chinese Journal of Applied and Environmental Biology, 2000, v. 6 n. 1, p. 48-51 How to Cite?
AbstractA rat Alu like repetitive sequence (RALRS 1) was cloned from rat low Cot DNA library. The cloned RALRS 1 was sequenced and found to be 283 bp in length containing highly repeated AG, AGG, and AGGG sequences. The copy number of RALRS 1sequence was around 150 000~330 000 per rat haploid genome. A synthesized 28 mer oligo nucleotide probe, e.g. (AGGG) 7 was used in the DNA fingerprinting analysis and resulted in a distinct banding pattern. Additionally, the differences of the banding pattern were seen between normal and tumor tissue in rat. This probe should be useful for the gene mutation caused by various factors in rat.
Persistent Identifierhttp://hdl.handle.net/10722/84829
ISSN
2015 SCImago Journal Rankings: 0.199

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorLeung, FCCen_HK
dc.contributor.authorCross, Fen_HK
dc.contributor.authorWelt, Men_HK
dc.contributor.authorTan, SJen_HK
dc.contributor.authorYang, SQen_HK
dc.date.accessioned2010-09-06T08:57:36Z-
dc.date.available2010-09-06T08:57:36Z-
dc.date.issued2000en_HK
dc.identifier.citation應用與環境生物學報, 2000, v. 6 n. 1, p. 48-51en_HK
dc.identifier.citationChinese Journal of Applied and Environmental Biology, 2000, v. 6 n. 1, p. 48-51-
dc.identifier.issn1006-687X-
dc.identifier.urihttp://hdl.handle.net/10722/84829-
dc.description.abstractA rat Alu like repetitive sequence (RALRS 1) was cloned from rat low Cot DNA library. The cloned RALRS 1 was sequenced and found to be 283 bp in length containing highly repeated AG, AGG, and AGGG sequences. The copy number of RALRS 1sequence was around 150 000~330 000 per rat haploid genome. A synthesized 28 mer oligo nucleotide probe, e.g. (AGGG) 7 was used in the DNA fingerprinting analysis and resulted in a distinct banding pattern. Additionally, the differences of the banding pattern were seen between normal and tumor tissue in rat. This probe should be useful for the gene mutation caused by various factors in rat.-
dc.languagechien_HK
dc.publisher科學出版社. The Journal's web site is located at http://www.cibj.com/-
dc.relation.ispartof應用與環境生物學報en_HK
dc.relation.ispartofChinese Journal of Applied and Environmental Biology-
dc.subjectRat genome-
dc.subjectLow Cot DNA-
dc.subjectAlu like sequence-
dc.subject(AGGG) 7 probe-
dc.titleCloning of an Alu-like Sequence from Rat Low-Cot DNA Library and Its Application in the Analysis of Genomic DNA Mutationen_HK
dc.title大鼠類Alu重復序列的克隆及其在染色體DNA突變分析中的應用-
dc.typeArticleen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.identifier.hkuros64357en_HK
dc.identifier.volume6-
dc.identifier.issue1-
dc.identifier.spage48-
dc.identifier.epage51-
dc.publisher.placeChina-

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