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Article: Gene Cloning and Sequence Analysis of VP5 for Chicken Infectious Bursal Disease Virus

TitleGene Cloning and Sequence Analysis of VP5 for Chicken Infectious Bursal Disease Virus
雞傳染性法氏囊病病毒VP5基因的克隆及其序列分析
Authors
KeywordsIBDV
VP5 gene
Cloning
Sequencing
Issue Date2001
Publisher山東大學. The Journal's web site is located at http://c.wanfangdata.com.cn/Periodical-sddxxb.aspx
Citation
山東大學學報(自然科學版), 2001, v. 36 n. 3, p. 346-350 How to Cite?
Journal of Shandong University (Natural Science Edition), 2001, v. 36 n. 3, p. 346-350 How to Cite?
AbstractChicken infectious bursal disease viruses (IBDV) were isolated and purified from infected bursa and then the viruses were used to extract genomic RNA.The cDNA first strand was synthesized by reverse transcription using IBDV genomic RNA as the template.A DNA fragment coding for the viral protein 5(VP5) of IBDV was amplified by PCR,which was ligated with pcDNA3.1(+) vector following restriction enzyme digestion.The ligates were transformed into XL1 blue and the recombinant plasmid pIBDV VP5 was screened and identified.The sequencing for VP5 gene and its sequence analysis were performed.
Persistent Identifierhttp://hdl.handle.net/10722/84819
ISSN

 

DC FieldValueLanguage
dc.contributor.authorLiu, CRen_HK
dc.contributor.authorYu, SQen_HK
dc.contributor.authorLeung, FCCen_HK
dc.date.accessioned2010-09-06T08:57:29Z-
dc.date.available2010-09-06T08:57:29Z-
dc.date.issued2001en_HK
dc.identifier.citation山東大學學報(自然科學版), 2001, v. 36 n. 3, p. 346-350en_HK
dc.identifier.citationJournal of Shandong University (Natural Science Edition), 2001, v. 36 n. 3, p. 346-350-
dc.identifier.issn1671-9352-
dc.identifier.urihttp://hdl.handle.net/10722/84819-
dc.description.abstractChicken infectious bursal disease viruses (IBDV) were isolated and purified from infected bursa and then the viruses were used to extract genomic RNA.The cDNA first strand was synthesized by reverse transcription using IBDV genomic RNA as the template.A DNA fragment coding for the viral protein 5(VP5) of IBDV was amplified by PCR,which was ligated with pcDNA3.1(+) vector following restriction enzyme digestion.The ligates were transformed into XL1 blue and the recombinant plasmid pIBDV VP5 was screened and identified.The sequencing for VP5 gene and its sequence analysis were performed.-
dc.languagechien_HK
dc.publisher山東大學. The Journal's web site is located at http://c.wanfangdata.com.cn/Periodical-sddxxb.aspx-
dc.relation.ispartof山東大學學報(自然科學版)en_HK
dc.relation.ispartofJournal of Shandong University (Natural Science Edition)-
dc.subjectIBDV-
dc.subjectVP5 gene-
dc.subjectCloning-
dc.subjectSequencing-
dc.titleGene Cloning and Sequence Analysis of VP5 for Chicken Infectious Bursal Disease Virusen_HK
dc.title雞傳染性法氏囊病病毒VP5基因的克隆及其序列分析-
dc.typeArticleen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.identifier.doi10.3969/j.issn.1671-9352.2001.03.019-
dc.identifier.hkuros85844en_HK
dc.identifier.volume36-
dc.identifier.issue3-
dc.identifier.spage346-
dc.identifier.epage350-
dc.publisher.placeChina-

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