File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Stability of epidermal growth factor in the gastrointestinal lumen of suckling and weaned pigs

TitleStability of epidermal growth factor in the gastrointestinal lumen of suckling and weaned pigs
Authors
Keywordsepidermal growth factor
gastrointestinal tract
milk
pig
Issue Date1996
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1996, v. 59 n. 3, p. 197-208 How to Cite?
AbstractThe stability of epidermal growth factor (EGF) in the gastrointestinal lumen of 3-day-old suckling and 45-day-old weaned pigs was examined by incubating iodine labeled recombinant human EGF (hEGF) in the gastrointestinal fluids at 37°C and monitoring the generation of acid soluble radioactivity. Chromatographic analysis and receptor binding study were also undertaken. The results showed that hEGF was quite stable in the gastric fluids in both suckling and weaned pigs with less than 20% degradation after 20 min incubation. The degradation rate in the intestinal fluids varied with the region of the small intestine and the age of the animals. At the proximal and mid regions of the small intestine in suckling pigs the degradation rate of hEGF after 20 min incubation was 5 and 20% respectively, while the degradation rate at the distal region was up to 50%. In the small intestinal lumen in weaned pigs the degradation rate of hEGF was much greater than that in suckling pigs, and the degradation rates at the proximal, mid and distal regions were 33, 51 and 56% respectively. Addition of acid soluble or casein fractions of porcine colostrum markedly reduced the degradation of hEGF in the intestinal fluids. These results indicate that milk-borne EGF is stable in the gastric and proximal intestinal lumen in suckling pigs, and may play a role in regulating postnatal development in the suckling young.
Persistent Identifierhttp://hdl.handle.net/10722/84818
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShen, WHen_HK
dc.contributor.authorXu, RJen_HK
dc.date.accessioned2010-09-06T08:57:28Z-
dc.date.available2010-09-06T08:57:28Z-
dc.date.issued1996en_HK
dc.identifier.citationLife Sciences, 1996, v. 59 n. 3, p. 197-208en_HK
dc.identifier.issn0024-3205en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84818-
dc.description.abstractThe stability of epidermal growth factor (EGF) in the gastrointestinal lumen of 3-day-old suckling and 45-day-old weaned pigs was examined by incubating iodine labeled recombinant human EGF (hEGF) in the gastrointestinal fluids at 37°C and monitoring the generation of acid soluble radioactivity. Chromatographic analysis and receptor binding study were also undertaken. The results showed that hEGF was quite stable in the gastric fluids in both suckling and weaned pigs with less than 20% degradation after 20 min incubation. The degradation rate in the intestinal fluids varied with the region of the small intestine and the age of the animals. At the proximal and mid regions of the small intestine in suckling pigs the degradation rate of hEGF after 20 min incubation was 5 and 20% respectively, while the degradation rate at the distal region was up to 50%. In the small intestinal lumen in weaned pigs the degradation rate of hEGF was much greater than that in suckling pigs, and the degradation rates at the proximal, mid and distal regions were 33, 51 and 56% respectively. Addition of acid soluble or casein fractions of porcine colostrum markedly reduced the degradation of hEGF in the intestinal fluids. These results indicate that milk-borne EGF is stable in the gastric and proximal intestinal lumen in suckling pigs, and may play a role in regulating postnatal development in the suckling young.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_HK
dc.relation.ispartofLife Sciencesen_HK
dc.rightsLife Sciences. Copyright © Elsevier Inc.en_HK
dc.subjectepidermal growth factoren_HK
dc.subjectgastrointestinal tracten_HK
dc.subjectmilken_HK
dc.subjectpigen_HK
dc.titleStability of epidermal growth factor in the gastrointestinal lumen of suckling and weaned pigsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0024-3205&volume=59&spage=197&epage=208&date=1996&atitle=Stability+of+epidermal+growth+factor+in+the+gastrointestinal+lumen+of+suckling+and+weaned+pigsen_HK
dc.identifier.emailXu, RJ: xuruojun@hkucc.hku.hken_HK
dc.identifier.authorityXu, RJ=rp00820en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0024-3205(96)00285-8en_HK
dc.identifier.pmid8699930-
dc.identifier.scopuseid_2-s2.0-0029998315en_HK
dc.identifier.hkuros20772en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029998315&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume59en_HK
dc.identifier.issue3en_HK
dc.identifier.spage197en_HK
dc.identifier.epage208en_HK
dc.identifier.isiWOS:A1996UQ74300002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShen, WH=46261460000en_HK
dc.identifier.scopusauthoridXu, RJ=7402813973en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats