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Article: Novel aspects of growth hormone (GH) autoregulation: GH-induced GH gene expression in grass carp pituitary cells through autocrine/paracrine mechanisms

TitleNovel aspects of growth hormone (GH) autoregulation: GH-induced GH gene expression in grass carp pituitary cells through autocrine/paracrine mechanisms
Authors
Issue Date2004
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2004, v. 145 n. 10, p. 4615-4628 How to Cite?
AbstractGH feedback on its own secretion at the pituitary level has been previously reported, but the mechanisms involved have not been elucidated. Here we examined the autocrine/paracrine effects of GH on GH synthesis using grass carp pituitary cells as a cell model. GH receptors were identified in carp somatotrophs, and their activation by exogenous GH increased steady-state GH mRNA levels and GH production. Removal of endogenous GH by immunoneutralization using GH antiserum inhibited basal as well as stimulated GH mRNA expression induced by GH-releasing factors in fish, including GnRH, apamorphine, and pituitary adenylate cyclase-activating polypeptide-38. Cytosolic mature GH mRNA levels were elevated by GH treatment and reduced by GH antiserum, whereas nuclear GH primary transcripts were almost undetectable after GH immunoneutralization. Inhibition of Janus kinase-2 (JAK 2), phosphoinositide 3-kinase, and MAPK also abolished GH-induced steady-state GH mRNA expression. GH immunoneutralization in pituitary cells pretreated with actinomycin D induced a marked decrease in the half-life of GH mRNA, indicating that the clearance of GH transcripts could be enhanced by removing endogenous GH. These results provide evidence that GH can serve as a novel intrapituitary autocrine/paracrine factor maintaining GH gene expression in somatotrophs, and this action is mediated by JAK 2/MAPK and JAK 2/ phosphoinositide 3-kinase cascades coupled to GH receptors.
Persistent Identifierhttp://hdl.handle.net/10722/84814
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Hen_HK
dc.contributor.authorKo, WKWen_HK
dc.contributor.authorHo, WKKen_HK
dc.contributor.authorStojilkovic, SSen_HK
dc.contributor.authorWong, AOLen_HK
dc.date.accessioned2010-09-06T08:57:25Z-
dc.date.available2010-09-06T08:57:25Z-
dc.date.issued2004en_HK
dc.identifier.citationEndocrinology, 2004, v. 145 n. 10, p. 4615-4628en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84814-
dc.description.abstractGH feedback on its own secretion at the pituitary level has been previously reported, but the mechanisms involved have not been elucidated. Here we examined the autocrine/paracrine effects of GH on GH synthesis using grass carp pituitary cells as a cell model. GH receptors were identified in carp somatotrophs, and their activation by exogenous GH increased steady-state GH mRNA levels and GH production. Removal of endogenous GH by immunoneutralization using GH antiserum inhibited basal as well as stimulated GH mRNA expression induced by GH-releasing factors in fish, including GnRH, apamorphine, and pituitary adenylate cyclase-activating polypeptide-38. Cytosolic mature GH mRNA levels were elevated by GH treatment and reduced by GH antiserum, whereas nuclear GH primary transcripts were almost undetectable after GH immunoneutralization. Inhibition of Janus kinase-2 (JAK 2), phosphoinositide 3-kinase, and MAPK also abolished GH-induced steady-state GH mRNA expression. GH immunoneutralization in pituitary cells pretreated with actinomycin D induced a marked decrease in the half-life of GH mRNA, indicating that the clearance of GH transcripts could be enhanced by removing endogenous GH. These results provide evidence that GH can serve as a novel intrapituitary autocrine/paracrine factor maintaining GH gene expression in somatotrophs, and this action is mediated by JAK 2/MAPK and JAK 2/ phosphoinositide 3-kinase cascades coupled to GH receptors.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsEndocrinology. Copyright © The Endocrine Society.en_HK
dc.titleNovel aspects of growth hormone (GH) autoregulation: GH-induced GH gene expression in grass carp pituitary cells through autocrine/paracrine mechanismsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=145&spage=4615&epage=4628&date=2004&atitle=Novel+Aspects+of+Growth+Hormone+(GH)+Autoregulation:+GH-Induced+GH+Gene+Expression+in+Grass+Carp+Pituitary+Cells+through+Autocrine/Paracrine+Mechanismsen_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2004-0163en_HK
dc.identifier.pmid15231712-
dc.identifier.scopuseid_2-s2.0-4644260394en_HK
dc.identifier.hkuros92347en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4644260394&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume145en_HK
dc.identifier.issue10en_HK
dc.identifier.spage4615en_HK
dc.identifier.epage4628en_HK
dc.identifier.isiWOS:000223885600024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhou, H=7404742310en_HK
dc.identifier.scopusauthoridKo, WKW=7202286890en_HK
dc.identifier.scopusauthoridHo, WKK=7402968862en_HK
dc.identifier.scopusauthoridStojilkovic, SS=7004268568en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK

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