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Article: Transcriptional profiling of Vero E6 cells over-expressing SARS-CoV S2 subunit: Insights on viral regulation of apoptosis and proliferation

TitleTranscriptional profiling of Vero E6 cells over-expressing SARS-CoV S2 subunit: Insights on viral regulation of apoptosis and proliferation
Authors
KeywordsApoptosis
Microarray
Proliferation
Severe acute respiratory syndrome coronavirus
Spike protein
Issue Date2008
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2008, v. 371 n. 1, p. 32-43 How to Cite?
AbstractWe have previously demonstrated that over-expression of spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) or its C-terminal subunit (S2) is sufficient to induce apoptosis in vitro. To further investigate the possible roles of S2 in SARS-CoV-induced apoptosis and pathogenesis of SARS, we characterized the host expression profiles induced upon S2 over-expression in Vero E6 cells by oligonucleotide microarray analysis. Possible activation of mitochondrial apoptotic pathway in S2 expressing cells was suggested, as evidenced by the up-regulation of cytochrome c and down-regulation of the Bcl-2 family anti-apoptotic members. Inhibition of Bcl-2-related anti-apoptotic pathway was further supported by the diminution of S2-induced apoptosis in Vero E6 cells over-expressing Bcl-xL. In addition, modulation of CCN E2 and CDKN 1A implied the possible control of cell cycle arrest at G1/S phase. This study is expected to extend our understanding on the pathogenesis of SARS at a molecular level. © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/84806
ISSN
2015 Impact Factor: 3.2
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, YSen_HK
dc.contributor.authorYip, CWen_HK
dc.contributor.authorHon, CCen_HK
dc.contributor.authorChow, KYCen_HK
dc.contributor.authorMa, ICMen_HK
dc.contributor.authorZeng, Fen_HK
dc.contributor.authorLeung, FCCen_HK
dc.date.accessioned2010-09-06T08:57:20Z-
dc.date.available2010-09-06T08:57:20Z-
dc.date.issued2008en_HK
dc.identifier.citationVirology, 2008, v. 371 n. 1, p. 32-43en_HK
dc.identifier.issn0042-6822en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84806-
dc.description.abstractWe have previously demonstrated that over-expression of spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) or its C-terminal subunit (S2) is sufficient to induce apoptosis in vitro. To further investigate the possible roles of S2 in SARS-CoV-induced apoptosis and pathogenesis of SARS, we characterized the host expression profiles induced upon S2 over-expression in Vero E6 cells by oligonucleotide microarray analysis. Possible activation of mitochondrial apoptotic pathway in S2 expressing cells was suggested, as evidenced by the up-regulation of cytochrome c and down-regulation of the Bcl-2 family anti-apoptotic members. Inhibition of Bcl-2-related anti-apoptotic pathway was further supported by the diminution of S2-induced apoptosis in Vero E6 cells over-expressing Bcl-xL. In addition, modulation of CCN E2 and CDKN 1A implied the possible control of cell cycle arrest at G1/S phase. This study is expected to extend our understanding on the pathogenesis of SARS at a molecular level. © 2007 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_HK
dc.relation.ispartofVirologyen_HK
dc.subjectApoptosisen_HK
dc.subjectMicroarrayen_HK
dc.subjectProliferationen_HK
dc.subjectSevere acute respiratory syndrome coronavirusen_HK
dc.subjectSpike proteinen_HK
dc.subject.meshApoptosis-
dc.subject.meshCell Proliferation-
dc.subject.meshMembrane Glycoproteins - chemistry - genetics - metabolism-
dc.subject.meshSevere Acute Respiratory Syndrome - virology-
dc.subject.meshViral Envelope Proteins - chemistry - genetics - metabolism-
dc.titleTranscriptional profiling of Vero E6 cells over-expressing SARS-CoV S2 subunit: Insights on viral regulation of apoptosis and proliferationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0042-6822&volume=371&issue=1&spage=32&epage=43&date=2008&atitle=Transcriptional+profiling+of+Vero+E6+cells+over-expressing+SARS-CoV+S2+subunit:+insights+on+viral+regulation+of+apoptosis+and+proliferationen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.virol.2007.09.016en_HK
dc.identifier.pmid17961624-
dc.identifier.scopuseid_2-s2.0-37849039252en_HK
dc.identifier.hkuros166182en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-37849039252&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume371en_HK
dc.identifier.issue1en_HK
dc.identifier.spage32en_HK
dc.identifier.epage43en_HK
dc.identifier.isiWOS:000252725700004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYeung, YS=9841205900en_HK
dc.identifier.scopusauthoridYip, CW=7101665559en_HK
dc.identifier.scopusauthoridHon, CC=7003617137en_HK
dc.identifier.scopusauthoridChow, KYC=7202180875en_HK
dc.identifier.scopusauthoridMa, ICM=23135024600en_HK
dc.identifier.scopusauthoridZeng, F=7202911544en_HK
dc.identifier.scopusauthoridLeung, FCC=7103078633en_HK

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