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Article: Low-Cot DNA sequences for fingerprinting analysis of germplasm diversity and relationships in Amaranthus

TitleLow-Cot DNA sequences for fingerprinting analysis of germplasm diversity and relationships in Amaranthus
Authors
KeywordsAmaranthus
DNA fingerprinting
Genetic diversity
Low-Cot DNA
Phylogenetic relationships
Issue Date1999
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00122/index.htm
Citation
Theoretical And Applied Genetics, 1999, v. 99 n. 3-4, p. 464-472 How to Cite?
AbstractWe examined genetic diversity and relationships among 24 cultivated and wild Amaranthus accessions using the total low-Cot DNA and five individual repetitive sequences as probes. These low-Cot DNA probes were obtained by the isolation of various classes of repetitive-DNA sequences, including satellites, minisatellites, microsatellites, rDNA, retrotransposon-like sequences, and other unidentified novel repetitive sequences. DNA fingerprints generated by different types of repetitive-DNA probes revealed different levels of polymorphism in the Amaranthus genomes. A repetitive sequence containing microsatellites was found to be a suitable probe for characterizing intraspecific accessions, whereas more conservative sequences (e.g. rDNA) were informative for resolving phylogenetic relationships among distantly related species. Genetic diversity, measured as restriction fragment length polymorphism (RFLP) and the similarity index at the low-Cot DNA level, was equally high among intraspecific accessions between the two species groups: grain amaranths (A. caudatus, A. cruentus, and A. hypochondriacus) and their putative wild progenitors (A. hybridus, A. powellii, anti A. quitensis). At the interspecific level, however, the grain amaranth species are less divergent from each other than their wild progenitors. With the rare exceptions of certain A. caudatus accessions, grain amaranths were found to be closely related to A. hybridus. The results based on low-Cot DNA were comparable with previous RAPD and isozyme studies of the same set of species/accessions of Amaranthus, indicating that low-Cot DNA sequences are suitable probes for a fingerprinting analysis of plant germplasm diversity and for determining phylogenetic relationships.
Persistent Identifierhttp://hdl.handle.net/10722/84805
ISSN
2015 Impact Factor: 3.9
2015 SCImago Journal Rankings: 1.930
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSun, Men_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2010-09-06T08:57:19Z-
dc.date.available2010-09-06T08:57:19Z-
dc.date.issued1999en_HK
dc.identifier.citationTheoretical And Applied Genetics, 1999, v. 99 n. 3-4, p. 464-472en_HK
dc.identifier.issn0040-5752en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84805-
dc.description.abstractWe examined genetic diversity and relationships among 24 cultivated and wild Amaranthus accessions using the total low-Cot DNA and five individual repetitive sequences as probes. These low-Cot DNA probes were obtained by the isolation of various classes of repetitive-DNA sequences, including satellites, minisatellites, microsatellites, rDNA, retrotransposon-like sequences, and other unidentified novel repetitive sequences. DNA fingerprints generated by different types of repetitive-DNA probes revealed different levels of polymorphism in the Amaranthus genomes. A repetitive sequence containing microsatellites was found to be a suitable probe for characterizing intraspecific accessions, whereas more conservative sequences (e.g. rDNA) were informative for resolving phylogenetic relationships among distantly related species. Genetic diversity, measured as restriction fragment length polymorphism (RFLP) and the similarity index at the low-Cot DNA level, was equally high among intraspecific accessions between the two species groups: grain amaranths (A. caudatus, A. cruentus, and A. hypochondriacus) and their putative wild progenitors (A. hybridus, A. powellii, anti A. quitensis). At the interspecific level, however, the grain amaranth species are less divergent from each other than their wild progenitors. With the rare exceptions of certain A. caudatus accessions, grain amaranths were found to be closely related to A. hybridus. The results based on low-Cot DNA were comparable with previous RAPD and isozyme studies of the same set of species/accessions of Amaranthus, indicating that low-Cot DNA sequences are suitable probes for a fingerprinting analysis of plant germplasm diversity and for determining phylogenetic relationships.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00122/index.htmen_HK
dc.relation.ispartofTheoretical and Applied Geneticsen_HK
dc.subjectAmaranthusen_HK
dc.subjectDNA fingerprintingen_HK
dc.subjectGenetic diversityen_HK
dc.subjectLow-Cot DNAen_HK
dc.subjectPhylogenetic relationshipsen_HK
dc.titleLow-Cot DNA sequences for fingerprinting analysis of germplasm diversity and relationships in Amaranthusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0040-5752&volume=99&spage=464&epage=472&date=1999&atitle=Low-Cot+DNA+Sequences+for+fingerprinting+Analysis+of+Germplasm+Diversity+and+Relationships+in+Amaranthusen_HK
dc.identifier.emailSun, M: meisun@hkucc.hku.hken_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authoritySun, M=rp00779en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s001220051258en_HK
dc.identifier.scopuseid_2-s2.0-0032876224en_HK
dc.identifier.hkuros43130en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032876224&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume99en_HK
dc.identifier.issue3-4en_HK
dc.identifier.spage464en_HK
dc.identifier.epage472en_HK
dc.identifier.isiWOS:000082570500011-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridSun, M=7403181447en_HK
dc.identifier.scopusauthoridChen, H=7501612240en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK
dc.identifier.citeulike4371348-

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