File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Functional antagonism of the human secretin receptor by a recombinant protein encoding the N-terminal ectodomain of the receptor.

TitleFunctional antagonism of the human secretin receptor by a recombinant protein encoding the N-terminal ectodomain of the receptor.
Authors
Issue Date1997
PublisherHumana Press, Inc. The Journal's web site is located at http://humanapress.com
Citation
Receptors & Signal Transduction, 1997, v. 7 n. 3, p. 143-150 How to Cite?
AbstractRecent evidence indicates that the N-terminal extracellular domain of receptors in the secretin-glucagon receptor family is responsible for ligand recognition. In this report, the N-terminal ectodomain of the human secretin receptor (HSR) was expressed in Escherichia coli, and the ability of this recombinant protein to interact with secretin was investigated by functional assays. The cDNA region encoding the N-terminal ectodomain of HSR linked to the polyhistidine fusion partner was expressed in E. coli. The resulting fusion protein was purified and used for competitive studies. A permanently transfected cell line with the HSR expressed was used in this study. The cell line was able to respond to secretin leading to the elevation of both intracellular cAMP and protein kinase-A activity. Using this cell line, incubation of secretin with the recombinant protein led to a dose-dependent inhibition of both cAMP production and protein kinase-A activity. These findings strongly suggested that the N-terminal ectodomain of HSR alone can act as a functional domain that provides a means to study ligand-receptor interactions of this receptor. The His-tagged recombinant HSR ectodomain may also be used for screening secretin-specific agonists and antagonists by affinity chromatography in the future.
Persistent Identifierhttp://hdl.handle.net/10722/84785
ISSN
2000 SCImago Journal Rankings: 0.762
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChow, BKen_HK
dc.date.accessioned2010-09-06T08:57:05Z-
dc.date.available2010-09-06T08:57:05Z-
dc.date.issued1997en_HK
dc.identifier.citationReceptors & Signal Transduction, 1997, v. 7 n. 3, p. 143-150en_HK
dc.identifier.issn1087-8475en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84785-
dc.description.abstractRecent evidence indicates that the N-terminal extracellular domain of receptors in the secretin-glucagon receptor family is responsible for ligand recognition. In this report, the N-terminal ectodomain of the human secretin receptor (HSR) was expressed in Escherichia coli, and the ability of this recombinant protein to interact with secretin was investigated by functional assays. The cDNA region encoding the N-terminal ectodomain of HSR linked to the polyhistidine fusion partner was expressed in E. coli. The resulting fusion protein was purified and used for competitive studies. A permanently transfected cell line with the HSR expressed was used in this study. The cell line was able to respond to secretin leading to the elevation of both intracellular cAMP and protein kinase-A activity. Using this cell line, incubation of secretin with the recombinant protein led to a dose-dependent inhibition of both cAMP production and protein kinase-A activity. These findings strongly suggested that the N-terminal ectodomain of HSR alone can act as a functional domain that provides a means to study ligand-receptor interactions of this receptor. The His-tagged recombinant HSR ectodomain may also be used for screening secretin-specific agonists and antagonists by affinity chromatography in the future.en_HK
dc.languageengen_HK
dc.publisherHumana Press, Inc. The Journal's web site is located at http://humanapress.comen_HK
dc.relation.ispartofReceptors & signal transductionen_HK
dc.titleFunctional antagonism of the human secretin receptor by a recombinant protein encoding the N-terminal ectodomain of the receptor.en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1087-8475&volume=7&spage=143&epage=150&date=1997&atitle=Functional+antagonism+of+the+human+secretin+receptor+by+a+recombinant+protein+encoding+the+N-terminal+ectodomain+of+the+receptoren_HK
dc.identifier.emailChow, BK: bkcc@hku.hken_HK
dc.identifier.authorityChow, BK=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9440501-
dc.identifier.scopuseid_2-s2.0-0031309624en_HK
dc.identifier.hkuros24442en_HK
dc.identifier.volume7en_HK
dc.identifier.issue3en_HK
dc.identifier.spage143en_HK
dc.identifier.epage150en_HK
dc.identifier.isiWOS:000071138000002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChow, BK=7102826193en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats