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Article: Identification and characterization of a receptor from goldfish specific for a teleost growth hormone-releasing hormone-like peptide

TitleIdentification and characterization of a receptor from goldfish specific for a teleost growth hormone-releasing hormone-like peptide
Authors
KeywordsFishes
Growth hormone-releasing hormone
Growth hormone-releasing hormone receptor
Molecular neuroendocrinology
Issue Date1998
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NEN
Citation
Neuroendocrinology, 1998, v. 68 n. 1, p. 44-56 How to Cite?
AbstractIn mammals, growth hormone-releasing hormone (GHRH), acting via the GHRH receptor, plays an important role in the regulation of growth hormone (GH) synthesis and secretion as well as the proliferation and differentiation of somatotropes in the pituitary. In fishes, information concerning the functional role of the characterized GHRHs is limited. For that reason, a putative goldfish GHRH receptor cDNA was characterized in this study. The receptor cDNA is 2243 bp in length, encoding a 438-amino-acid-long polypeptide with 7 putative transmembrane-spanning regions, which is a characteristic of G-protein-coupled receptors. The receptor, when expressed in COS-7 cells, showed minimal responses (2-fold cAMP responses) when stimulated with 100 nM of human GHRH, pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP). However, this receptor was found to be specific for a carp GHRH-like peptide isolated from the brain of common carp (Cyprinus carpio); there was a significant and dose-dependent increase in intracellular cAMP (a maximum response of 22-fold increase with an EC 50 Of 0.1 nM) when the transfected cells were stimulated with this peptide. As a preliminary study to investigate the functional role of this receptor, the tissue distribution of the mRNA was analyzed by reverse-transcription-polymerase chain reaction. The receptor mRNA was found to be present in the brain, pituitary, gut, gill, heart, liver, skeletal muscle, spleen, ovary and testis. Together with a goldfish PACAP type 1 receptor and a VIP1 receptor recently isolated in our laboratory, characterization of this putative GHRH receptor provides the molecular basis for the future understanding of the neuroendocrine control of growth and reproduction by these neuropeptides in goldfish as well as other teleosts.
Persistent Identifierhttp://hdl.handle.net/10722/84778
ISSN
2015 Impact Factor: 2.583
2015 SCImago Journal Rankings: 1.479
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, KWen_HK
dc.contributor.authorYu, KLen_HK
dc.contributor.authorRivier, Jen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:57:00Z-
dc.date.available2010-09-06T08:57:00Z-
dc.date.issued1998en_HK
dc.identifier.citationNeuroendocrinology, 1998, v. 68 n. 1, p. 44-56en_HK
dc.identifier.issn0028-3835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84778-
dc.description.abstractIn mammals, growth hormone-releasing hormone (GHRH), acting via the GHRH receptor, plays an important role in the regulation of growth hormone (GH) synthesis and secretion as well as the proliferation and differentiation of somatotropes in the pituitary. In fishes, information concerning the functional role of the characterized GHRHs is limited. For that reason, a putative goldfish GHRH receptor cDNA was characterized in this study. The receptor cDNA is 2243 bp in length, encoding a 438-amino-acid-long polypeptide with 7 putative transmembrane-spanning regions, which is a characteristic of G-protein-coupled receptors. The receptor, when expressed in COS-7 cells, showed minimal responses (2-fold cAMP responses) when stimulated with 100 nM of human GHRH, pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP). However, this receptor was found to be specific for a carp GHRH-like peptide isolated from the brain of common carp (Cyprinus carpio); there was a significant and dose-dependent increase in intracellular cAMP (a maximum response of 22-fold increase with an EC 50 Of 0.1 nM) when the transfected cells were stimulated with this peptide. As a preliminary study to investigate the functional role of this receptor, the tissue distribution of the mRNA was analyzed by reverse-transcription-polymerase chain reaction. The receptor mRNA was found to be present in the brain, pituitary, gut, gill, heart, liver, skeletal muscle, spleen, ovary and testis. Together with a goldfish PACAP type 1 receptor and a VIP1 receptor recently isolated in our laboratory, characterization of this putative GHRH receptor provides the molecular basis for the future understanding of the neuroendocrine control of growth and reproduction by these neuropeptides in goldfish as well as other teleosts.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NENen_HK
dc.relation.ispartofNeuroendocrinologyen_HK
dc.rightsNeuroendocrinology. Copyright © S Karger AG.en_HK
dc.subjectFishesen_HK
dc.subjectGrowth hormone-releasing hormoneen_HK
dc.subjectGrowth hormone-releasing hormone receptoren_HK
dc.subjectMolecular neuroendocrinologyen_HK
dc.titleIdentification and characterization of a receptor from goldfish specific for a teleost growth hormone-releasing hormone-like peptideen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0028-3835&volume=68&spage=44&epage=56&date=1998&atitle=Identification+and+characterization+of+a+receptor+from+goldfish+specific+for+a+teleost+growth+hormone-releasing+hormone-like+peptideen_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000054349en_HK
dc.identifier.pmid9695938-
dc.identifier.scopuseid_2-s2.0-0031861337en_HK
dc.identifier.hkuros34159en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031861337&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume68en_HK
dc.identifier.issue1en_HK
dc.identifier.spage44en_HK
dc.identifier.epage56en_HK
dc.identifier.isiWOS:000074845700006-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridChan, KW=55041303600en_HK
dc.identifier.scopusauthoridYu, KL=7403385265en_HK
dc.identifier.scopusauthoridRivier, J=35411857000en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK

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