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Article: Identification of the endogenous ligands for chicken growth hormone-releasing hormone (GHRH) receptor: Evidence for a separate gene encoding GHRH in submammalian vertebrates

TitleIdentification of the endogenous ligands for chicken growth hormone-releasing hormone (GHRH) receptor: Evidence for a separate gene encoding GHRH in submammalian vertebrates
Authors
Issue Date2007
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2007, v. 148 n. 5, p. 2405-2416 How to Cite?
AbstractIt is generally believed that hypothalamic GHRH activates GHRH receptor (GHRHR) to stimulate GH synthesis and release in the pituitary of mammals. However, the identity of the endogenous ligand of GHRHR is still unresolved in submammalian vertebrates including birds. In this study, we have successfully identified the chicken GHRH (cGHRH) gene, which consists of seven exons including two exons (exons 4 and 5) coding for the predicted mature GHRH peptide of 47 amino acids. Interestingly, the differential usage of splice donor sites at exon 6 results in the generation of two prepro-GHRHs (172 and 188 amino acids in length) with different C-terminal tails. Similar to mammals, cGHRH was detected to be predominantly expressed in the hypothalamus by RT-PCR assay. Using the pGL3-CRE-luciferase reporter system, we further demonstrated that both the synthetic cGHRH peptides (cGHRH1-47 and cGHRH1-31) and conditioned medium from CHO cells expressing cGHRH could strongly induce luciferase activity via activation of cGHRHR, indicating that cGHRH could bind cGHRHR and activate downstream cAMP-protein kinase A signaling pathway. Using the same system, cGHRH-like peptide was also shown to be capable of activating cGHRHR in vitro. As in chicken, a conserved GHRH gene was identified in the genomes of lower vertebrate species including zebrafish, fugu, tetraodon, and Xenopus by synteny analysis. Collectively, our data suggest that GHRH, perhaps together with GHRH-like peptide (chicken/carp-like), may function as the authentic endogenous ligands of GHRHR in chicken as well as in other lower vertebrate species. Copyright © 2007 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/84773
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorWang, CYen_HK
dc.contributor.authorKwok, AHYen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2010-09-06T08:56:57Z-
dc.date.available2010-09-06T08:56:57Z-
dc.date.issued2007en_HK
dc.identifier.citationEndocrinology, 2007, v. 148 n. 5, p. 2405-2416en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84773-
dc.description.abstractIt is generally believed that hypothalamic GHRH activates GHRH receptor (GHRHR) to stimulate GH synthesis and release in the pituitary of mammals. However, the identity of the endogenous ligand of GHRHR is still unresolved in submammalian vertebrates including birds. In this study, we have successfully identified the chicken GHRH (cGHRH) gene, which consists of seven exons including two exons (exons 4 and 5) coding for the predicted mature GHRH peptide of 47 amino acids. Interestingly, the differential usage of splice donor sites at exon 6 results in the generation of two prepro-GHRHs (172 and 188 amino acids in length) with different C-terminal tails. Similar to mammals, cGHRH was detected to be predominantly expressed in the hypothalamus by RT-PCR assay. Using the pGL3-CRE-luciferase reporter system, we further demonstrated that both the synthetic cGHRH peptides (cGHRH1-47 and cGHRH1-31) and conditioned medium from CHO cells expressing cGHRH could strongly induce luciferase activity via activation of cGHRHR, indicating that cGHRH could bind cGHRHR and activate downstream cAMP-protein kinase A signaling pathway. Using the same system, cGHRH-like peptide was also shown to be capable of activating cGHRHR in vitro. As in chicken, a conserved GHRH gene was identified in the genomes of lower vertebrate species including zebrafish, fugu, tetraodon, and Xenopus by synteny analysis. Collectively, our data suggest that GHRH, perhaps together with GHRH-like peptide (chicken/carp-like), may function as the authentic endogenous ligands of GHRHR in chicken as well as in other lower vertebrate species. Copyright © 2007 by The Endocrine Society.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsEndocrinology. Copyright © The Endocrine Society.en_HK
dc.titleIdentification of the endogenous ligands for chicken growth hormone-releasing hormone (GHRH) receptor: Evidence for a separate gene encoding GHRH in submammalian vertebratesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=148&spage=2405&epage=2416&date=2007&atitle=Identification+of+the+Endogenous+Ligands+for+Chicken+Growth+Hormone-Releasing+Hormone+(GHRH)+Receptor:+Evidence+for+a+Separate+Gene+Encoding+GHRH+in+Submammalian+Vertebratesen_HK
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00801en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2006-1013en_HK
dc.identifier.pmid17272401-
dc.identifier.scopuseid_2-s2.0-34249795802en_HK
dc.identifier.hkuros133515en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249795802&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume148en_HK
dc.identifier.issue5en_HK
dc.identifier.spage2405en_HK
dc.identifier.epage2416en_HK
dc.identifier.isiWOS:000245810600051-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, Y=36062525200en_HK
dc.identifier.scopusauthoridLi, J=27171035300en_HK
dc.identifier.scopusauthoridWang, CY=16065173000en_HK
dc.identifier.scopusauthoridKwok, AHY=27168105100en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK

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