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Article: Expression and localization of Smad1, Smad2 and Smad4 proteins in rat testis during postnatal development

TitleExpression and localization of Smad1, Smad2 and Smad4 proteins in rat testis during postnatal development
Authors
KeywordsSmad1
Smad2
Smad4
Testis
TGF-β
Issue Date2003
PublisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=1008-682X&site=1
Citation
Asian Journal Of Andrology, 2003, v. 5 n. 1, p. 51-55 How to Cite?
AbstractAim: To study the expression and regulation of Smad1, Smad2 and Smad4 proteins (intracellular signaling molecules of transforming growth factor-β family) in rat testis during postnatal development. Methods: The whole testes were collected from SD rats aged 3, 7, 14, 28 and 90 (adult) days. The cellular localization and developmental changes were examined by immunohistochemistry ABC method with the glucose oxidase-DAB-nickel enhancement technique. Quantitative analysis of the immunostaining was made by the image analysis system. The Smads proteins coexistence in the adult rat testis was tested by the double immune staining for CD14-Smad4 and Smad2-Smad4. The protein expression of Smad during rat testicular development was examined by means of Western blots. Results: Smad1, Smad2 and Smad4 were present throughout testicular development. The immunostaining of Smad1 and Smad2 were present in spermatogenic cells. A positive immunoreactivity was located at the cytoplasm, but the nucleus was negative. Smad1 was immunolocalized at the d14, d28 and adult testes, while Smad2, at the d7, d14, d28 and adult testis. There was positive immunoreaction in the Sertoli cells and Leydig cells as well. The immunolocalization of Smad4 was exclusively at the cytoplasm of Leydig cells and the nuclei were negative throughout the testicular development. No expression was detected in the germ cells. The results of image and statistical analysis showed that generally the expression of Smad1, Smad2 and Smad4 in the testis tended to increase gradually with the growth of the rat. Conclusion: The present data provide direct evidences for the molecular mechanism of TGF-β action in rat testes during postnatal development and spermatogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/84765
ISSN
2015 Impact Factor: 2.644
2015 SCImago Journal Rankings: 0.879
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, Jen_HK
dc.contributor.authorZhang, YQen_HK
dc.contributor.authorLiu, XPen_HK
dc.contributor.authorWang, RAen_HK
dc.contributor.authorJin, Yen_HK
dc.contributor.authorXu, RJen_HK
dc.date.accessioned2010-09-06T08:56:51Z-
dc.date.available2010-09-06T08:56:51Z-
dc.date.issued2003en_HK
dc.identifier.citationAsian Journal Of Andrology, 2003, v. 5 n. 1, p. 51-55en_HK
dc.identifier.issn1008-682Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84765-
dc.description.abstractAim: To study the expression and regulation of Smad1, Smad2 and Smad4 proteins (intracellular signaling molecules of transforming growth factor-β family) in rat testis during postnatal development. Methods: The whole testes were collected from SD rats aged 3, 7, 14, 28 and 90 (adult) days. The cellular localization and developmental changes were examined by immunohistochemistry ABC method with the glucose oxidase-DAB-nickel enhancement technique. Quantitative analysis of the immunostaining was made by the image analysis system. The Smads proteins coexistence in the adult rat testis was tested by the double immune staining for CD14-Smad4 and Smad2-Smad4. The protein expression of Smad during rat testicular development was examined by means of Western blots. Results: Smad1, Smad2 and Smad4 were present throughout testicular development. The immunostaining of Smad1 and Smad2 were present in spermatogenic cells. A positive immunoreactivity was located at the cytoplasm, but the nucleus was negative. Smad1 was immunolocalized at the d14, d28 and adult testes, while Smad2, at the d7, d14, d28 and adult testis. There was positive immunoreaction in the Sertoli cells and Leydig cells as well. The immunolocalization of Smad4 was exclusively at the cytoplasm of Leydig cells and the nuclei were negative throughout the testicular development. No expression was detected in the germ cells. The results of image and statistical analysis showed that generally the expression of Smad1, Smad2 and Smad4 in the testis tended to increase gradually with the growth of the rat. Conclusion: The present data provide direct evidences for the molecular mechanism of TGF-β action in rat testes during postnatal development and spermatogenesis.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=1008-682X&site=1en_HK
dc.relation.ispartofAsian Journal of Andrologyen_HK
dc.subjectSmad1en_HK
dc.subjectSmad2en_HK
dc.subjectSmad4en_HK
dc.subjectTestisen_HK
dc.subjectTGF-βen_HK
dc.titleExpression and localization of Smad1, Smad2 and Smad4 proteins in rat testis during postnatal developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1008-682X&volume=5&spage=51&epage=55&date=2003&atitle=Expression+and+localization+of+Smad1,+Smad2+and+Smad4+proteins+in+rat+testis+during+postnatal+developmenten_HK
dc.identifier.emailXu, RJ: xuruojun@hkucc.hku.hken_HK
dc.identifier.authorityXu, RJ=rp00820en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12647004-
dc.identifier.scopuseid_2-s2.0-0037357892en_HK
dc.identifier.hkuros76816en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037357892&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue1en_HK
dc.identifier.spage51en_HK
dc.identifier.epage55en_HK
dc.identifier.isiWOS:000182119500010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHu, J=7406418707en_HK
dc.identifier.scopusauthoridZhang, YQ=7601321843en_HK
dc.identifier.scopusauthoridLiu, XP=8572202600en_HK
dc.identifier.scopusauthoridWang, RA=36071739300en_HK
dc.identifier.scopusauthoridJin, Y=26643271200en_HK
dc.identifier.scopusauthoridXu, RJ=7402813973en_HK

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