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Article: The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor

TitleThe culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor
Authors
KeywordsCoriolus versicolor
Flow cytometry
High performance liquid chromatography
Molt 4 leukemia
Peripheral blood mononuclear cells
Polysaccharopeptide
Issue Date2006
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictec
Citation
Enzyme And Microbial Technology, 2006, v. 38 n. 1-2, p. 14-21 How to Cite?
AbstractPolysaccharopeptide (PSP) derives from the medicinal mushroom Coriolus versicolor is considered a biological response modifier with potential pharmaceutical applications. Significant literatures support the immune and anticancer functions of PSP; however, standardization is of big concern because variable biotechnological factors can affect both the chemical and biological properties of PSP. In this study, the extracts of PSP obtained at different days from the Coriolus versicolor culture were tested in vitro for their immune function on human normal peripheral blood mononuclear cells (PBMC) and cytotoxicity on the human leukemia Molt 4 cells. Over the 10-days culture period, both biomass and peptide/polysaccharide content were increased with time. The increase in proliferation index of PBMC and their production of interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α) and gamma interferon (IFN-γ) in the presence of PHA strengthens the correlation between culture duration and biological potency of PSP. The growth inhibition of the Molt 4 cells by PSP also depended on its maturity. Flow cytometry analysis on cell cycle and cell death (apoptosis) of Molt 4 cells indicated that the anticancer mechanism of PSP is related to its ability to induce S-phase cell arrest and apoptosis, respectively. Together, these results suggest that monitor the harvest duration is critical for the quality control of polysaccharopeptide in the biotechnological industry. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/84760
ISSN
2015 Impact Factor: 2.624
2015 SCImago Journal Rankings: 0.854
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, CLen_HK
dc.contributor.authorYang, Xen_HK
dc.contributor.authorWan, JMFen_HK
dc.date.accessioned2010-09-06T08:56:48Z-
dc.date.available2010-09-06T08:56:48Z-
dc.date.issued2006en_HK
dc.identifier.citationEnzyme And Microbial Technology, 2006, v. 38 n. 1-2, p. 14-21en_HK
dc.identifier.issn0141-0229en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84760-
dc.description.abstractPolysaccharopeptide (PSP) derives from the medicinal mushroom Coriolus versicolor is considered a biological response modifier with potential pharmaceutical applications. Significant literatures support the immune and anticancer functions of PSP; however, standardization is of big concern because variable biotechnological factors can affect both the chemical and biological properties of PSP. In this study, the extracts of PSP obtained at different days from the Coriolus versicolor culture were tested in vitro for their immune function on human normal peripheral blood mononuclear cells (PBMC) and cytotoxicity on the human leukemia Molt 4 cells. Over the 10-days culture period, both biomass and peptide/polysaccharide content were increased with time. The increase in proliferation index of PBMC and their production of interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α) and gamma interferon (IFN-γ) in the presence of PHA strengthens the correlation between culture duration and biological potency of PSP. The growth inhibition of the Molt 4 cells by PSP also depended on its maturity. Flow cytometry analysis on cell cycle and cell death (apoptosis) of Molt 4 cells indicated that the anticancer mechanism of PSP is related to its ability to induce S-phase cell arrest and apoptosis, respectively. Together, these results suggest that monitor the harvest duration is critical for the quality control of polysaccharopeptide in the biotechnological industry. © 2005 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictecen_HK
dc.relation.ispartofEnzyme and Microbial Technologyen_HK
dc.rightsEnzyme and Microbial Technology. Copyright © Elsevier Inc.en_HK
dc.subjectCoriolus versicoloren_HK
dc.subjectFlow cytometryen_HK
dc.subjectHigh performance liquid chromatographyen_HK
dc.subjectMolt 4 leukemiaen_HK
dc.subjectPeripheral blood mononuclear cellsen_HK
dc.subjectPolysaccharopeptideen_HK
dc.titleThe culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicoloren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-0229&volume=38&spage=14&epage=21&date=2006&atitle=The+culture+duration+affects+the+immunomodulatory+and+anticancer+effect+of+polysaccharopeptide+derived+from+Coriolus+versicoloren_HK
dc.identifier.emailWan, JMF: jmfwan@hku.hken_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.enzmictec.2004.10.009en_HK
dc.identifier.scopuseid_2-s2.0-27844552489en_HK
dc.identifier.hkuros123606en_HK
dc.identifier.hkuros171461-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27844552489&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage14en_HK
dc.identifier.epage21en_HK
dc.identifier.isiWOS:000234199300003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, CL=9277221100en_HK
dc.identifier.scopusauthoridYang, X=14014496500en_HK
dc.identifier.scopusauthoridWan, JMF=8930305000en_HK

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