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Article: Identification of functional domains on gC1Q-R, a cell surface protein that binds to the globular 'heads' of C1Q, using monoclonal antibodies and synthetic peptides

TitleIdentification of functional domains on gC1Q-R, a cell surface protein that binds to the globular 'heads' of C1Q, using monoclonal antibodies and synthetic peptides
Authors
Issue Date1996
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=20
Citation
Hybridoma, 1996, v. 15 n. 5, p. 333-342 How to Cite?
AbstractA membrane protein (33 kDa) that binds to the globular 'heads' of C1q (gC1q-R) has been recently described. The full length cDNA encoding gC1q-R has been cloned, expressed in E. coli and using the purified recombinant protein (rgC1q-R) as an immunogen, a panel of IgG monoclonal antibodies (MAb) has been produced by fusion of spleen cells from hyperimmunized BALB/c mice with NSO mouse myeloma partners. From this fusion, 60 anti-gC1q-R hybridomas were selected and evaluated for their ability to (1) discriminate between the mature form (MF) of gC1q-R (residues 74-282) and a truncated form (TF) lacking residues 74-95, which contains a major C1q binding site, (2) recognize two functionally defined synthetic peptides derived from the NH 2- (XN 18) and COOH-(XC 15) terminus of gC1q-R, and (3) bind to microtiter well fixed intact Raji cells. Several clones were identified: MAbs 46.23 and 60.11 (IgG(1κ)), reacted strongly with ELISA plate-fixed intact Raji and K562 cells, MF, and the XN 18 peptide, but had poor or no reactivity with TF; MAbs 74.5.2 > 25.15 (IgG(1κ)) recognized both MF and TF and are directed against epitopes in the XC 15 peptide that contains a binding site for high- molecular-weight kininogen and Factor XII.
Persistent Identifierhttp://hdl.handle.net/10722/84758
ISSN
2003 Impact Factor: 0.509
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGhebrehiwet, Ben_HK
dc.contributor.authorLu, PDen_HK
dc.contributor.authorZhang, Wen_HK
dc.contributor.authorLim, BLen_HK
dc.contributor.authorEggleton, Pen_HK
dc.contributor.authorLeigh, LEAen_HK
dc.contributor.authorReid, KBMen_HK
dc.contributor.authorPeerschke, EIBen_HK
dc.date.accessioned2010-09-06T08:56:46Z-
dc.date.available2010-09-06T08:56:46Z-
dc.date.issued1996en_HK
dc.identifier.citationHybridoma, 1996, v. 15 n. 5, p. 333-342en_HK
dc.identifier.issn0272-457Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84758-
dc.description.abstractA membrane protein (33 kDa) that binds to the globular 'heads' of C1q (gC1q-R) has been recently described. The full length cDNA encoding gC1q-R has been cloned, expressed in E. coli and using the purified recombinant protein (rgC1q-R) as an immunogen, a panel of IgG monoclonal antibodies (MAb) has been produced by fusion of spleen cells from hyperimmunized BALB/c mice with NSO mouse myeloma partners. From this fusion, 60 anti-gC1q-R hybridomas were selected and evaluated for their ability to (1) discriminate between the mature form (MF) of gC1q-R (residues 74-282) and a truncated form (TF) lacking residues 74-95, which contains a major C1q binding site, (2) recognize two functionally defined synthetic peptides derived from the NH 2- (XN 18) and COOH-(XC 15) terminus of gC1q-R, and (3) bind to microtiter well fixed intact Raji cells. Several clones were identified: MAbs 46.23 and 60.11 (IgG(1κ)), reacted strongly with ELISA plate-fixed intact Raji and K562 cells, MF, and the XN 18 peptide, but had poor or no reactivity with TF; MAbs 74.5.2 > 25.15 (IgG(1κ)) recognized both MF and TF and are directed against epitopes in the XC 15 peptide that contains a binding site for high- molecular-weight kininogen and Factor XII.en_HK
dc.languageengen_HK
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=20en_HK
dc.relation.ispartofHybridomaen_HK
dc.titleIdentification of functional domains on gC1Q-R, a cell surface protein that binds to the globular 'heads' of C1Q, using monoclonal antibodies and synthetic peptidesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1554-0014&volume=15&spage=333&epage=342&date=1996&atitle=Identification+of+functional+domains+on+gC1Q-R,+a+cell+surface+protein+that+binds+to+the+globular+%27heads%27+of+C1Q,+using+monoclonal+antibodies+and+synthetic+peptidesen_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8913782-
dc.identifier.scopuseid_2-s2.0-0029822011en_HK
dc.identifier.hkuros21991en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029822011&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue5en_HK
dc.identifier.spage333en_HK
dc.identifier.epage342en_HK
dc.identifier.isiWOS:A1996VR16600001-
dc.identifier.scopusauthoridGhebrehiwet, B=7005039582en_HK
dc.identifier.scopusauthoridLu, PD=55041420900en_HK
dc.identifier.scopusauthoridZhang, W=7409434223en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK
dc.identifier.scopusauthoridEggleton, P=7006176421en_HK
dc.identifier.scopusauthoridLeigh, LEA=6701775303en_HK
dc.identifier.scopusauthoridReid, KBM=7202780648en_HK
dc.identifier.scopusauthoridPeerschke, EIB=7005991940en_HK

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