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Article: Expression of grass carp growth hormone by baculovirus in silkworm larvae

TitleExpression of grass carp growth hormone by baculovirus in silkworm larvae
Authors
KeywordsBaculovirus
Bombyx mori
Fish
Growth hormone
Silkworm
Issue Date1998
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbagen
Citation
Biochimica Et Biophysica Acta - General Subjects, 1998, v. 1381 n. 3, p. 331-339 How to Cite?
AbstractA total of five recombinant Bombyx mori nuclear polyhedrosis viruses (BMNPV) carrying the grass carp (Ctenopharyngodon idellus) growth hormone (GH) cDNA were constructed in this study. Two of them were able to express the hormone up to a level of 12 μg/ml medium when cultured B. mori cells were infected for 4 days. Inoculation of the viruses into silkworm (B. mori) host significantly increased the level of GH achievable. The amount of hormone produced per larva was estimated to be around 1 mg. The recombinant grass carp GH had immunological and biological activities similar to the native hormone. The N-terminal sequence of the recombinant hormone was the same as the native one, indicating that the fish signal peptide was correctly processed by the insect cells. Silkworm powder prepared from larvae infected with the recombinant virus was used as food supplement for fish. Compared with the control, this dietary supplement was effective in increasing the growth rate of juvenile carp. Copyright (C) 1998 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/84756
ISSN
2015 Impact Factor: 5.083
2015 SCImago Journal Rankings: 2.128
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, WKKen_HK
dc.contributor.authorMeng, ZQen_HK
dc.contributor.authorLin, HRen_HK
dc.contributor.authorPoon, CTen_HK
dc.contributor.authorLeung, YKen_HK
dc.contributor.authorYan, KTen_HK
dc.contributor.authorDias, Nen_HK
dc.contributor.authorChe, APKen_HK
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorZheng, WMen_HK
dc.contributor.authorSun, Yen_HK
dc.contributor.authorWong, AOLen_HK
dc.date.accessioned2010-09-06T08:56:45Z-
dc.date.available2010-09-06T08:56:45Z-
dc.date.issued1998en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - General Subjects, 1998, v. 1381 n. 3, p. 331-339en_HK
dc.identifier.issn0304-4165en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84756-
dc.description.abstractA total of five recombinant Bombyx mori nuclear polyhedrosis viruses (BMNPV) carrying the grass carp (Ctenopharyngodon idellus) growth hormone (GH) cDNA were constructed in this study. Two of them were able to express the hormone up to a level of 12 μg/ml medium when cultured B. mori cells were infected for 4 days. Inoculation of the viruses into silkworm (B. mori) host significantly increased the level of GH achievable. The amount of hormone produced per larva was estimated to be around 1 mg. The recombinant grass carp GH had immunological and biological activities similar to the native hormone. The N-terminal sequence of the recombinant hormone was the same as the native one, indicating that the fish signal peptide was correctly processed by the insect cells. Silkworm powder prepared from larvae infected with the recombinant virus was used as food supplement for fish. Compared with the control, this dietary supplement was effective in increasing the growth rate of juvenile carp. Copyright (C) 1998 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbagenen_HK
dc.relation.ispartofBiochimica et Biophysica Acta - General Subjectsen_HK
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.en_HK
dc.subjectBaculovirusen_HK
dc.subjectBombyx morien_HK
dc.subjectFishen_HK
dc.subjectGrowth hormoneen_HK
dc.subjectSilkwormen_HK
dc.titleExpression of grass carp growth hormone by baculovirus in silkworm larvaeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3002&volume=1381&spage=331&epage=339&date=1998&atitle=Expression+of+grass+carp+growth+hormone+by+baculovirus+in+silkworm+larvaeen_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0304-4165(98)00044-0en_HK
dc.identifier.pmid9729443-
dc.identifier.scopuseid_2-s2.0-0031811907en_HK
dc.identifier.hkuros43214en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031811907&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1381en_HK
dc.identifier.issue3en_HK
dc.identifier.spage331en_HK
dc.identifier.epage339en_HK
dc.identifier.isiWOS:000075793600008-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHo, WKK=7402968862en_HK
dc.identifier.scopusauthoridMeng, ZQ=35620681300en_HK
dc.identifier.scopusauthoridLin, HR=7405571313en_HK
dc.identifier.scopusauthoridPoon, CT=36922576900en_HK
dc.identifier.scopusauthoridLeung, YK=14050649700en_HK
dc.identifier.scopusauthoridYan, KT=7102869266en_HK
dc.identifier.scopusauthoridDias, N=7005734415en_HK
dc.identifier.scopusauthoridChe, APK=36932297900en_HK
dc.identifier.scopusauthoridLiu, J=25824364500en_HK
dc.identifier.scopusauthoridZheng, WM=36638976300en_HK
dc.identifier.scopusauthoridSun, Y=8899511700en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK

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