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Article: In situ localization of infectious bursal disease virus-binding cells by a biotin-streptavidin system

TitleIn situ localization of infectious bursal disease virus-binding cells by a biotin-streptavidin system
Authors
KeywordsBiotin-streptavidin system
IBDV-binding cells
Infectious bursal disease virus
Issue Date2001
PublisherAmerican Association of Avian Pathologists, Inc. The Journal's web site is located at http://avdi.allenpress.com/avdionline/?request=index-html
Citation
Avian Diseases, 2001, v. 45 n. 2, p. 504-511 How to Cite?
AbstractA biotin-streptavidin system was established to directly visualize infectious bursal disease virus (IBDV)-binding cells in cell culture or in fresh tissues. The cells or tissue sections were first incubated with a biotinylated, purified IBDV strain GZ911 and then with a streptavidin-β-galactosidase conjugate. In the presence of the enzyme substrate X-gal, IBDV-binding cells were labeled in blue color. By applying this method to frozen tissue sections, virus-binding sites were localized in situ in the bursa, spleen, and kidney tissue sections, whereas no positive cells were detected in the thymus tissue sections. Chicken embryo fibroblasts, Veto cells, MOP-8 cells, 293-EBNA cells, PANC-1 cells, and HuTu 80 cells were found to bind to the virus. However, the binding of the virus to MDA-MB-231 cells and SVG p12 cells was undetectable. This method can be employed for the expressional cloning of IBDV receptor and can be applied to studies on other avian viruses.
Persistent Identifierhttp://hdl.handle.net/10722/84753
ISSN
2015 Impact Factor: 1.104
2015 SCImago Journal Rankings: 0.772
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXue, CYen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2010-09-06T08:56:43Z-
dc.date.available2010-09-06T08:56:43Z-
dc.date.issued2001en_HK
dc.identifier.citationAvian Diseases, 2001, v. 45 n. 2, p. 504-511en_HK
dc.identifier.issn0005-2086en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84753-
dc.description.abstractA biotin-streptavidin system was established to directly visualize infectious bursal disease virus (IBDV)-binding cells in cell culture or in fresh tissues. The cells or tissue sections were first incubated with a biotinylated, purified IBDV strain GZ911 and then with a streptavidin-β-galactosidase conjugate. In the presence of the enzyme substrate X-gal, IBDV-binding cells were labeled in blue color. By applying this method to frozen tissue sections, virus-binding sites were localized in situ in the bursa, spleen, and kidney tissue sections, whereas no positive cells were detected in the thymus tissue sections. Chicken embryo fibroblasts, Veto cells, MOP-8 cells, 293-EBNA cells, PANC-1 cells, and HuTu 80 cells were found to bind to the virus. However, the binding of the virus to MDA-MB-231 cells and SVG p12 cells was undetectable. This method can be employed for the expressional cloning of IBDV receptor and can be applied to studies on other avian viruses.en_HK
dc.languageengen_HK
dc.publisherAmerican Association of Avian Pathologists, Inc. The Journal's web site is located at http://avdi.allenpress.com/avdionline/?request=index-htmlen_HK
dc.relation.ispartofAvian Diseasesen_HK
dc.subjectBiotin-streptavidin systemen_HK
dc.subjectIBDV-binding cellsen_HK
dc.subjectInfectious bursal disease virusen_HK
dc.titleIn situ localization of infectious bursal disease virus-binding cells by a biotin-streptavidin systemen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0005-2086&volume=45&spage=504&epage=511&date=2001&atitle=In+Situ+Localization+of+Infectious+Bursal+Disease+Virus-Binding+Cells+by+a+Biotin-Streptavidin+Systemen_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11417836-
dc.identifier.scopuseid_2-s2.0-0034965696en_HK
dc.identifier.hkuros57447en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034965696&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue2en_HK
dc.identifier.spage504en_HK
dc.identifier.epage511en_HK
dc.identifier.isiWOS:000169271700030-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXue, CY=55178736400en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK

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