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Article: Expression and localization of Smad4 protein in rat testis during the postnatal development

TitleExpression and localization of Smad4 protein in rat testis during the postnatal development
Authors
KeywordsSmad4
Testis
Transforming growth factor
Issue Date2003
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/10019294.asp
Citation
Chinese Medical Sciences Journal, 2003, v. 18 n. 3, p. 155-161 How to Cite?
AbstractObjective. To study expression and localization of Smad4 protein, the common-mediator Smad, which is one of intracellular signaling molecules of transforming growth factor-β (TGF-β) family, in rat testis during postnatal development. Methods. In this study, whole testes were collected from S.D rats aged 3 days, 7 days, 14 days and 28 days, and adult respectively. We examined the cellular localization and developmental change of Smad4 in rat testis by immunohistochemical ABC method with glucose oxidase-DAB-nickel enhancement technique; the quantitative analysis of the immunostaining by the image analysis system; the Smads proteins coexistence in the adult rat testis by the double immune staining for CD14 - Smad4 and Smad2 - Smad4; the protein expression of Smad during rat testicular development by means of Western blots. Results. The protein of Smad4 was present in rats from 3 days of age to adulthood, and the immunolocalization was exclusively localized to the cytoplasm of Leydig cells with negative nuclei in the interstitial tissue at any time point. No expression was detected in germ cells. The result of image and statistical analysis showed that generally, there was a tendency that the expression of Smad4 in the testes increased gradually with the rats developing maturation. Conclusion. Our data provide direct evidence for the molecular mechanism of TGF-β action in rat testes during postnatal development and spermatogenesis of rats.
Persistent Identifierhttp://hdl.handle.net/10722/84740
ISSN
2015 SCImago Journal Rankings: 0.272
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorHu, Jen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorXu, Ren_HK
dc.date.accessioned2010-09-06T08:56:34Z-
dc.date.available2010-09-06T08:56:34Z-
dc.date.issued2003en_HK
dc.identifier.citationChinese Medical Sciences Journal, 2003, v. 18 n. 3, p. 155-161en_HK
dc.identifier.issn1001-9294en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84740-
dc.description.abstractObjective. To study expression and localization of Smad4 protein, the common-mediator Smad, which is one of intracellular signaling molecules of transforming growth factor-β (TGF-β) family, in rat testis during postnatal development. Methods. In this study, whole testes were collected from S.D rats aged 3 days, 7 days, 14 days and 28 days, and adult respectively. We examined the cellular localization and developmental change of Smad4 in rat testis by immunohistochemical ABC method with glucose oxidase-DAB-nickel enhancement technique; the quantitative analysis of the immunostaining by the image analysis system; the Smads proteins coexistence in the adult rat testis by the double immune staining for CD14 - Smad4 and Smad2 - Smad4; the protein expression of Smad during rat testicular development by means of Western blots. Results. The protein of Smad4 was present in rats from 3 days of age to adulthood, and the immunolocalization was exclusively localized to the cytoplasm of Leydig cells with negative nuclei in the interstitial tissue at any time point. No expression was detected in germ cells. The result of image and statistical analysis showed that generally, there was a tendency that the expression of Smad4 in the testes increased gradually with the rats developing maturation. Conclusion. Our data provide direct evidence for the molecular mechanism of TGF-β action in rat testes during postnatal development and spermatogenesis of rats.en_HK
dc.languageengen_HK
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/10019294.aspen_HK
dc.relation.ispartofChinese Medical Sciences Journalen_HK
dc.rightsChinese Medical Sciences Journal. Copyright © Informa Healthcare.en_HK
dc.subjectSmad4en_HK
dc.subjectTestisen_HK
dc.subjectTransforming growth factoren_HK
dc.titleExpression and localization of Smad4 protein in rat testis during the postnatal developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1001-9294&volume=18&spage=155&epage=161&date=2003&atitle=Expression+and+Localization+of+SMAD4+Protein+in+Rat+Testis+during+the+Postnatal+Developmenten_HK
dc.identifier.emailXu, R: xuruojun@hkucc.hku.hken_HK
dc.identifier.authorityXu, R=rp00820en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0141792282en_HK
dc.identifier.hkuros92083en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141792282&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue3en_HK
dc.identifier.spage155en_HK
dc.identifier.epage161en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhang, Y=7601321843en_HK
dc.identifier.scopusauthoridHu, J=7406418707en_HK
dc.identifier.scopusauthoridLiu, X=8572202600en_HK
dc.identifier.scopusauthoridXu, R=7402813973en_HK

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