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Article: Characterization of human cytoglobin gene promoter region

TitleCharacterization of human cytoglobin gene promoter region
Authors
Keywordsc-Ets-1
Cytoglobin (CYGB)
Promoter region
Sp1
Transcription factor
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
Citation
Biochimica Et Biophysica Acta - Gene Structure And Expression, 2006, v. 1759 n. 5, p. 208-215 How to Cite?
AbstractCytoglobin (CYGB) is a member of the vertebrate globin family together with hemoglobin, myoglobin and neuroglobin. Although the physiological function of CYGB is still unclear, spectroscopic studies show that CYGB contains a hexacoordinated heme pocket similar to other pentacoordinated globin proteins. CYGB shares a common phylogenetic ancestry with vertebrate myoglobin from which it diverged by duplication before the appearance of jawed vertebrates. The objective of this study is to identify the regulatory and promoter region of the human cytoglobin gene. 5′ unidirectional deletion constructs demonstrated that the proximal promoter elements of human CYGB gene are located between -1113 to -10 relative to the translation start site. Site-directed mutagenesis showed that mutation of a c-Ets-1 motif at -1008 and Sp1 motifs at -400, -230 and -210 remarkably decreased the promoter activity. Gel shift assays confirmed the binding of DNA-nuclear proteins to these motifs. All these results indicate that CYGB gene expression can be up-regulated by c-Ets-1 and Sp1 motifs. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/84728
ISSN
2007 Impact Factor: 1.704
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuo, Xen_HK
dc.contributor.authorPhilipsen, Sen_HK
dc.contributor.authorTanUn, KCen_HK
dc.date.accessioned2010-09-06T08:56:25Z-
dc.date.available2010-09-06T08:56:25Z-
dc.date.issued2006en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2006, v. 1759 n. 5, p. 208-215en_HK
dc.identifier.issn0167-4781en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84728-
dc.description.abstractCytoglobin (CYGB) is a member of the vertebrate globin family together with hemoglobin, myoglobin and neuroglobin. Although the physiological function of CYGB is still unclear, spectroscopic studies show that CYGB contains a hexacoordinated heme pocket similar to other pentacoordinated globin proteins. CYGB shares a common phylogenetic ancestry with vertebrate myoglobin from which it diverged by duplication before the appearance of jawed vertebrates. The objective of this study is to identify the regulatory and promoter region of the human cytoglobin gene. 5′ unidirectional deletion constructs demonstrated that the proximal promoter elements of human CYGB gene are located between -1113 to -10 relative to the translation start site. Site-directed mutagenesis showed that mutation of a c-Ets-1 motif at -1008 and Sp1 motifs at -400, -230 and -210 remarkably decreased the promoter activity. Gel shift assays confirmed the binding of DNA-nuclear proteins to these motifs. All these results indicate that CYGB gene expression can be up-regulated by c-Ets-1 and Sp1 motifs. © 2006 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexpen_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Gene Structure and Expressionen_HK
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.en_HK
dc.subjectc-Ets-1en_HK
dc.subjectCytoglobin (CYGB)en_HK
dc.subjectPromoter regionen_HK
dc.subjectSp1en_HK
dc.subjectTranscription factoren_HK
dc.titleCharacterization of human cytoglobin gene promoter regionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3002&volume=1759&spage=208&epage=215&date=2006&atitle=Characterization+of+human+cytoglobin+gene+promoter+regionen_HK
dc.identifier.emailTanUn, KC: kctanun@hkucc.hku.hken_HK
dc.identifier.authorityTanUn, KC=rp00787en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbaexp.2006.04.002en_HK
dc.identifier.pmid16797742-
dc.identifier.scopuseid_2-s2.0-33745919556en_HK
dc.identifier.hkuros120942en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33745919556&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1759en_HK
dc.identifier.issue5en_HK
dc.identifier.spage208en_HK
dc.identifier.epage215en_HK
dc.identifier.isiWOS:000239611200002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridGuo, X=36725898900en_HK
dc.identifier.scopusauthoridPhilipsen, S=7003745052en_HK
dc.identifier.scopusauthoridTanUn, KC=6602914262en_HK

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