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Article: Serotonin increases the excitability of the hypothalamic paraventricular nucleus magnocellular neurons

TitleSerotonin increases the excitability of the hypothalamic paraventricular nucleus magnocellular neurons
Authors
Keywords5-HT1B receptor
5-HT2C receptor
Patch-clamp
Rat
Issue Date2007
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJN
Citation
European Journal Of Neuroscience, 2007, v. 25 n. 10, p. 2991-3000 How to Cite?
AbstractRecent evidence that 5-hydroxytryptamine (5-HT or serotonin) enhances the release and the gene expression of vasopressin and oxytocin in the hypothalamic paraventricular nucleus (PVN) suggests that 5-HT can excite the PVN magnocellular neurons. The objective of this study was to examine the underlying mechanisms for such excitatory action in the electrophysiologically identified hypothalamic PVN magnocellular neurons in rats using whole-cell patch-clamp. We found that 5-HT weakly depolarizes 33.3% of PVN magnocellular neurons in the presence of tetrodotoxin. A minuscule inward current was produced by 5-HT in 48% of the cells, which was attenuated when the 5-HT4 antagonist GR113808 or the 5-HT7 antagonist SB269970 was added. In addition, 5-HT reduced the frequency of miniature inhibitory postsynaptic currents in a dose-dependent manner. This inhibition was mimicked by the 5-HT1B agonist CP93129, and reversed in the presence of 5-HT1B antagonists cyanopindolol and SB224289. Besides, 5-HT induced a biphasic effect on the frequency of miniature excitatory postsynaptic currents, comprising a transient inhibition and a delayed concentration-dependent excitation (onset latency ∼ 5 min). The facilitation was mimicked by the 5-HT2A/2C agonist DOI and abolished in the presence of the 5-HT2C antagonist RS102221. Our findings reveal that 5-HT directly increases the excitability of the PVN magnocellular neurons via multiple receptor subtypes and mechanisms. This may help understanding the regulation of 5-HT-induced hormone release and feeding behavior in the PVN. © The Authors (2007).
Persistent Identifierhttp://hdl.handle.net/10722/84725
ISSN
2015 Impact Factor: 2.975
2015 SCImago Journal Rankings: 2.130
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, SSNen_HK
dc.contributor.authorChow, BKCen_HK
dc.contributor.authorYung, WHen_HK
dc.date.accessioned2010-09-06T08:56:23Z-
dc.date.available2010-09-06T08:56:23Z-
dc.date.issued2007en_HK
dc.identifier.citationEuropean Journal Of Neuroscience, 2007, v. 25 n. 10, p. 2991-3000en_HK
dc.identifier.issn0953-816Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84725-
dc.description.abstractRecent evidence that 5-hydroxytryptamine (5-HT or serotonin) enhances the release and the gene expression of vasopressin and oxytocin in the hypothalamic paraventricular nucleus (PVN) suggests that 5-HT can excite the PVN magnocellular neurons. The objective of this study was to examine the underlying mechanisms for such excitatory action in the electrophysiologically identified hypothalamic PVN magnocellular neurons in rats using whole-cell patch-clamp. We found that 5-HT weakly depolarizes 33.3% of PVN magnocellular neurons in the presence of tetrodotoxin. A minuscule inward current was produced by 5-HT in 48% of the cells, which was attenuated when the 5-HT4 antagonist GR113808 or the 5-HT7 antagonist SB269970 was added. In addition, 5-HT reduced the frequency of miniature inhibitory postsynaptic currents in a dose-dependent manner. This inhibition was mimicked by the 5-HT1B agonist CP93129, and reversed in the presence of 5-HT1B antagonists cyanopindolol and SB224289. Besides, 5-HT induced a biphasic effect on the frequency of miniature excitatory postsynaptic currents, comprising a transient inhibition and a delayed concentration-dependent excitation (onset latency ∼ 5 min). The facilitation was mimicked by the 5-HT2A/2C agonist DOI and abolished in the presence of the 5-HT2C antagonist RS102221. Our findings reveal that 5-HT directly increases the excitability of the PVN magnocellular neurons via multiple receptor subtypes and mechanisms. This may help understanding the regulation of 5-HT-induced hormone release and feeding behavior in the PVN. © The Authors (2007).en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJNen_HK
dc.relation.ispartofEuropean Journal of Neuroscienceen_HK
dc.rightsEuropean Journal of Neuroscience. Copyright © Blackwell Publishing Ltd.en_HK
dc.subject5-HT1B receptoren_HK
dc.subject5-HT2C receptoren_HK
dc.subjectPatch-clampen_HK
dc.subjectRaten_HK
dc.titleSerotonin increases the excitability of the hypothalamic paraventricular nucleus magnocellular neuronsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0953-816X&volume=25&spage=2991&epage=3000&date=2007&atitle=Serotonin+increases+the+excitability+of+the+hypothalamic+paraventricular+nucleus+magnocellular+neuronsen_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1460-9568.2007.05547.xen_HK
dc.identifier.pmid17561813-
dc.identifier.scopuseid_2-s2.0-34249977895en_HK
dc.identifier.hkuros129397en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249977895&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue10en_HK
dc.identifier.spage2991en_HK
dc.identifier.epage3000en_HK
dc.identifier.isiWOS:000247115300006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHo, SSN=16443947000en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK
dc.identifier.scopusauthoridYung, WH=7103137893en_HK

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