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Article: Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray.

TitleMolecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray.
Authors
Issue Date2005
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology., 2005, v. 27 n. 2, p. 513-523 How to Cite?
AbstractProteins and peptide bound polysaccharides (PSP) extracted from Basidiomycetous fungi are widely used in cancer immunotherapy and recently demonstrated to induce apoptosis in cancer cells in vitro. In order to provide the molecular pharmacological mechanisms of PSP on human cancer cells, we investigated the gene expression profiles of PSP-treated apoptotic human promyelotic leukemic HL-60 cells using ResGen 40k IMAGE printed cDNA microarray. In total 378 and 111 transcripts were identified as differentially expressed in the apoptotic cells by at least a factor of 2 or 3, respectively. Our data show that PSP-induced apoptosis in HL-60 cells might be mediated by up-regulation of early transcription factors such as AP-1, EGR1, IER2 and IER5, and down-regulation of NF-kappaB transcription pathways. Other gene expression changes, including the increase of several apoptotic or anti-proliferation genes, such as GADD45A/B and TUSC2, and the decrease of a batch of phosphatase and kinase genes, may also provide further evidences in supporting the process of PSP induced apoptosis in cancer cells. Some of the well-characterized carcinogenesis-related gene transcripts such as SAT, DCT, Melan-A, uPA and cyclin E1 were also alternated by PSP in the HL-60 cells. These transcripts can be employed as markers for quality control of PSP products on functional levels. The present study provides new insight into the molecular mechanisms involved in PSP-induced apoptosis in leukemic HL-60 cells analyzed by cDNA microarray.
Persistent Identifierhttp://hdl.handle.net/10722/84724
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZeng, Fen_HK
dc.contributor.authorHon, CCen_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorChow, KYen_HK
dc.contributor.authorHui, RKen_HK
dc.contributor.authorLaw, IKen_HK
dc.contributor.authorNg, VWen_HK
dc.contributor.authorYang, XTen_HK
dc.contributor.authorLeung, FCen_HK
dc.contributor.authorWan, JMen_HK
dc.date.accessioned2010-09-06T08:56:23Z-
dc.date.available2010-09-06T08:56:23Z-
dc.date.issued2005en_HK
dc.identifier.citationInternational Journal Of Oncology., 2005, v. 27 n. 2, p. 513-523en_HK
dc.identifier.issn1019-6439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84724-
dc.description.abstractProteins and peptide bound polysaccharides (PSP) extracted from Basidiomycetous fungi are widely used in cancer immunotherapy and recently demonstrated to induce apoptosis in cancer cells in vitro. In order to provide the molecular pharmacological mechanisms of PSP on human cancer cells, we investigated the gene expression profiles of PSP-treated apoptotic human promyelotic leukemic HL-60 cells using ResGen 40k IMAGE printed cDNA microarray. In total 378 and 111 transcripts were identified as differentially expressed in the apoptotic cells by at least a factor of 2 or 3, respectively. Our data show that PSP-induced apoptosis in HL-60 cells might be mediated by up-regulation of early transcription factors such as AP-1, EGR1, IER2 and IER5, and down-regulation of NF-kappaB transcription pathways. Other gene expression changes, including the increase of several apoptotic or anti-proliferation genes, such as GADD45A/B and TUSC2, and the decrease of a batch of phosphatase and kinase genes, may also provide further evidences in supporting the process of PSP induced apoptosis in cancer cells. Some of the well-characterized carcinogenesis-related gene transcripts such as SAT, DCT, Melan-A, uPA and cyclin E1 were also alternated by PSP in the HL-60 cells. These transcripts can be employed as markers for quality control of PSP products on functional levels. The present study provides new insight into the molecular mechanisms involved in PSP-induced apoptosis in leukemic HL-60 cells analyzed by cDNA microarray.en_HK
dc.languageengen_HK
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_HK
dc.relation.ispartofInternational journal of oncology.en_HK
dc.subject.meshApoptosis - drug effects - genetics-
dc.subject.meshBasidiomycota - chemistry-
dc.subject.meshGene Expression Regulation, Neoplastic - drug effects - genetics-
dc.subject.meshOligonucleotide Array Sequence Analysis - methods-
dc.subject.meshProteoglycans - isolation and purification - pharmacology-
dc.titleMolecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray.en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1019-6439&volume=27&issue=2&spage=513&epage=523&date=2005&atitle=Molecular+characterization+of+Coriolus+versicolor+PSP-induced+apoptosis+in+human+promyelotic+leukemic+HL-60+cells+using+cDNA+microarrayen_HK
dc.identifier.emailHui, RK: rkhhui@hkucc.hku.hken_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.emailWan, JM: jmfwan@hku.hken_HK
dc.identifier.authorityHui, RK=rp00711en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.identifier.authorityWan, JM=rp00798en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid16010435-
dc.identifier.scopuseid_2-s2.0-33644677526en_HK
dc.identifier.hkuros138640en_HK
dc.identifier.volume27en_HK
dc.identifier.issue2en_HK
dc.identifier.spage513en_HK
dc.identifier.epage523en_HK
dc.identifier.isiWOS:000230548500027-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridZeng, F=7202911544en_HK
dc.identifier.scopusauthoridHon, CC=7003617137en_HK
dc.identifier.scopusauthoridSit, WH=8528923000en_HK
dc.identifier.scopusauthoridChow, KY=7202180875en_HK
dc.identifier.scopusauthoridHui, RK=7103304764en_HK
dc.identifier.scopusauthoridLaw, IK=12764291700en_HK
dc.identifier.scopusauthoridNg, VW=7102162937en_HK
dc.identifier.scopusauthoridYang, XT=14014496500en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK
dc.identifier.scopusauthoridWan, JM=8930305000en_HK

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