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Article: Cloning and characterization of chicken growth hormone binding protein (cGHBP)

TitleCloning and characterization of chicken growth hormone binding protein (cGHBP)
Authors
KeywordsAlternative polyadenylation
cGHBP
cGHR
Genomic structure
Issue Date2007
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/domaniend
Citation
Domestic Animal Endocrinology, 2007, v. 33 n. 1, p. 107-121 How to Cite?
AbstractGrowth hormone (GH) is indispensable for the growth of animals and its biological activity is mediated by binding to the growth hormone receptor (GHR) [Harvey S, Scanes CG, Daughaday WH. Growth hormone. Boca Raton: CRC Press; 1995]. GHR is a transmembrane protein responsible for signal transduction upon GH binding. GH also binds to the growth hormone binding protein (GHBP) which is the soluble form of GHR extracellular domain existing in circulation. Actions of GHBP include prolongation of GH bioavailability and prevention of GH signaling system from over-stimulation. To date, little is known about the mechanisms generating the chicken GHBP (cGHBP). Elucidating the genomic structure of cGHR will provide insights into such underlying mechanisms. Using polymerase chain reaction and library screening methods, we have characterized the genomic organization of chicken GHR (cGHR). The full-length coding region of the cGHR transcript is composed of eight exons (exons 2-10), lacking a human homolog exon 3 and spans at least 71 kb on the genome. A novel transcript of size 1.2 kb was isolated from chicken liver total RNA using 5′ and 3′ rapid cDNA ends amplification (RACE). It was generated by utilizing a previously unknown polyadenylation signal located at the intron 6. Semi-quantitative reverse transcription polymerase chain reaction showed that this transcript is widely expressed in a variety of tissues. This transcript has an open reading frame comprising 203 amino acids. In vitro binding assay using ELISA demonstrated that Escherichia coli expressed recombinant protein encoded by this transcript was able to bind with chicken GH. Hence, this transcript is a potential candidate for cGHBP. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/84718
ISSN
2015 Impact Factor: 1.613
2015 SCImago Journal Rankings: 0.751
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, JSen_HK
dc.contributor.authorYip, CWen_HK
dc.contributor.authorLaw, KMen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2010-09-06T08:56:19Z-
dc.date.available2010-09-06T08:56:19Z-
dc.date.issued2007en_HK
dc.identifier.citationDomestic Animal Endocrinology, 2007, v. 33 n. 1, p. 107-121en_HK
dc.identifier.issn0739-7240en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84718-
dc.description.abstractGrowth hormone (GH) is indispensable for the growth of animals and its biological activity is mediated by binding to the growth hormone receptor (GHR) [Harvey S, Scanes CG, Daughaday WH. Growth hormone. Boca Raton: CRC Press; 1995]. GHR is a transmembrane protein responsible for signal transduction upon GH binding. GH also binds to the growth hormone binding protein (GHBP) which is the soluble form of GHR extracellular domain existing in circulation. Actions of GHBP include prolongation of GH bioavailability and prevention of GH signaling system from over-stimulation. To date, little is known about the mechanisms generating the chicken GHBP (cGHBP). Elucidating the genomic structure of cGHR will provide insights into such underlying mechanisms. Using polymerase chain reaction and library screening methods, we have characterized the genomic organization of chicken GHR (cGHR). The full-length coding region of the cGHR transcript is composed of eight exons (exons 2-10), lacking a human homolog exon 3 and spans at least 71 kb on the genome. A novel transcript of size 1.2 kb was isolated from chicken liver total RNA using 5′ and 3′ rapid cDNA ends amplification (RACE). It was generated by utilizing a previously unknown polyadenylation signal located at the intron 6. Semi-quantitative reverse transcription polymerase chain reaction showed that this transcript is widely expressed in a variety of tissues. This transcript has an open reading frame comprising 203 amino acids. In vitro binding assay using ELISA demonstrated that Escherichia coli expressed recombinant protein encoded by this transcript was able to bind with chicken GH. Hence, this transcript is a potential candidate for cGHBP. © 2006 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/domanienden_HK
dc.relation.ispartofDomestic Animal Endocrinologyen_HK
dc.rightsDomestic Animal Endocrinology. Copyright © Elsevier Inc.en_HK
dc.subjectAlternative polyadenylationen_HK
dc.subjectcGHBPen_HK
dc.subjectcGHRen_HK
dc.subjectGenomic structureen_HK
dc.titleCloning and characterization of chicken growth hormone binding protein (cGHBP)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0739-7240&volume=33&spage=107&epage=121&date=2007&atitle=Cloning+and+characterization+of+chicken+growth+hormone+binding+protein+(cGHBP)en_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.domaniend.2006.04.012en_HK
dc.identifier.pmid16814975-
dc.identifier.scopuseid_2-s2.0-34249816778en_HK
dc.identifier.hkuros133445en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249816778&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume33en_HK
dc.identifier.issue1en_HK
dc.identifier.spage107en_HK
dc.identifier.epage121en_HK
dc.identifier.isiWOS:000247358800008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, JS=35080638800en_HK
dc.identifier.scopusauthoridYip, CW=7101665559en_HK
dc.identifier.scopusauthoridLaw, KM=7202563042en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK

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