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Article: Cloning of Precursor Polyprotein Gene of Infectious Bursal Disease Virus by One Step

TitleCloning of Precursor Polyprotein Gene of Infectious Bursal Disease Virus by One Step
一步法克隆傳染性法氏囊病病毒前體多聚蛋白基因
Authors
KeywordsInfectious bursal disease virus
Precursor polyprotein gene
Cloning
Issue Date2001
Publisher中國微生物學會. The Journal's web site is located at http://bdxb.periodicals.net.cn/
Citation
病毒學報, 2001, v. 17 n. 2, p. 180-182 How to Cite?
Chinese Journal of Virology, 2001, v. 17 n. 2, p. 180-182 How to Cite?
AbstractVery virulent infectious bursal disease virus(vvIBDV)was isolated from chicken bursa and then the virus double stranded RNA was extracted After denaturation of dsRNA by heat in the presence of primers,the cDNA was synthesized by use of a reverse transcriptase lacking RNase H activity The RNA component of RNA cDNA hybrids was digested by RNase H By using an optimized PCR,a 3 05kb DNA fragment coding for precursor polyprotein of IBDV was produced in one step,which was inserted into pcDNA3 1(+) vector Two recombinant plasmids (pPP1 and pPP2)were screened and identified from eight XL1 blue colonies Partial sequencing for pPP1 plasmid indicated that the precursor polyprotein gene for IBDV was cloned successfully The method can simplify greatly the procedure to clone precursor polyprotein gene of IBDV isolates.
Persistent Identifierhttp://hdl.handle.net/10722/84712
ISSN
2015 SCImago Journal Rankings: 0.192

 

DC FieldValueLanguage
dc.contributor.authorLiu, CRen_HK
dc.contributor.authorLeung, FCCen_HK
dc.date.accessioned2010-09-06T08:56:14Z-
dc.date.available2010-09-06T08:56:14Z-
dc.date.issued2001en_HK
dc.identifier.citation病毒學報, 2001, v. 17 n. 2, p. 180-182en_HK
dc.identifier.citationChinese Journal of Virology, 2001, v. 17 n. 2, p. 180-182-
dc.identifier.issn1000-8721-
dc.identifier.urihttp://hdl.handle.net/10722/84712-
dc.description.abstractVery virulent infectious bursal disease virus(vvIBDV)was isolated from chicken bursa and then the virus double stranded RNA was extracted After denaturation of dsRNA by heat in the presence of primers,the cDNA was synthesized by use of a reverse transcriptase lacking RNase H activity The RNA component of RNA cDNA hybrids was digested by RNase H By using an optimized PCR,a 3 05kb DNA fragment coding for precursor polyprotein of IBDV was produced in one step,which was inserted into pcDNA3 1(+) vector Two recombinant plasmids (pPP1 and pPP2)were screened and identified from eight XL1 blue colonies Partial sequencing for pPP1 plasmid indicated that the precursor polyprotein gene for IBDV was cloned successfully The method can simplify greatly the procedure to clone precursor polyprotein gene of IBDV isolates.-
dc.languagechien_HK
dc.publisher中國微生物學會. The Journal's web site is located at http://bdxb.periodicals.net.cn/-
dc.relation.ispartof病毒學報en_HK
dc.relation.ispartofChinese Journal of Virology-
dc.subjectInfectious bursal disease virus-
dc.subjectPrecursor polyprotein gene-
dc.subjectCloning-
dc.titleCloning of Precursor Polyprotein Gene of Infectious Bursal Disease Virus by One Stepen_HK
dc.title一步法克隆傳染性法氏囊病病毒前體多聚蛋白基因-
dc.typeArticleen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.identifier.doi10.3321/j.issn:1000-8721.2001.02.019-
dc.identifier.hkuros85845en_HK
dc.identifier.volume17-
dc.identifier.issue2-
dc.identifier.spage180-
dc.identifier.epage182-
dc.publisher.placeChina-

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