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Article: Construction of plastid transient expression vector of GFP gene and its expression in E. Coli

TitleConstruction of plastid transient expression vector of GFP gene and its expression in E. Coli
綠色熒光蛋白質體瞬間表達載體的構建及在大腸桿菌中的表達
Authors
KeywordsGreen fluorescent protein (GFP)
psbA promoter
Plastid transient expression vector
Issue Date1997
Publisher中國農業大學. The Journal's web site is located at http://mall.cnki.net/magazine/magalist/NYSB.htm
Citation
農業生物技術學報, 1997, v. 5 n. 4, p. 339-345 How to Cite?
Journal of Agricultural Biotechnology, 1997, v. 5 n. 4, p. 339-345 How to Cite?
AbstractGreen fluorescent protein (GFP) has recently been developed as a monitoring marker for gene expression and protein localization in living organisms In this study,a plastid transient expression vector, pJGFP, was constructed by replacing the coding region of psbA gene with the GFP cDNA gene.The GFP was highly expressed in Escherichia coli DH5α under the control of the promoter of psbA gene,and the protein was readily detected by fluorescent spectrophotometer. The results show that GFP expressed in E.coli is capable of producing a strong green fluorescence (wavelength 509 nm), when excited by blue light (wavelength 395 nm). The GFP expressed in E.coli could also be seen in SDS-PAGE In this paper, we have also discussed the potential applicationof the plastid transient expression vector pJGFP as monitoring marker for plastid transformation
Persistent Identifierhttp://hdl.handle.net/10722/84705
ISSN

 

DC FieldValueLanguage
dc.contributor.authorFang, Xen_HK
dc.contributor.authorJin, Yen_HK
dc.contributor.authorSun, Men_HK
dc.date.accessioned2010-09-06T08:56:10Z-
dc.date.available2010-09-06T08:56:10Z-
dc.date.issued1997en_HK
dc.identifier.citation農業生物技術學報, 1997, v. 5 n. 4, p. 339-345en_HK
dc.identifier.citationJournal of Agricultural Biotechnology, 1997, v. 5 n. 4, p. 339-345-
dc.identifier.issn1006-1304en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84705-
dc.description.abstractGreen fluorescent protein (GFP) has recently been developed as a monitoring marker for gene expression and protein localization in living organisms In this study,a plastid transient expression vector, pJGFP, was constructed by replacing the coding region of psbA gene with the GFP cDNA gene.The GFP was highly expressed in Escherichia coli DH5α under the control of the promoter of psbA gene,and the protein was readily detected by fluorescent spectrophotometer. The results show that GFP expressed in E.coli is capable of producing a strong green fluorescence (wavelength 509 nm), when excited by blue light (wavelength 395 nm). The GFP expressed in E.coli could also be seen in SDS-PAGE In this paper, we have also discussed the potential applicationof the plastid transient expression vector pJGFP as monitoring marker for plastid transformation-
dc.languagechien_HK
dc.publisher中國農業大學. The Journal's web site is located at http://mall.cnki.net/magazine/magalist/NYSB.htmzh_HK
dc.relation.ispartof農業生物技術學報en_HK
dc.relation.ispartofJournal of Agricultural Biotechnology-
dc.subjectGreen fluorescent protein (GFP)-
dc.subjectpsbA promoter-
dc.subjectPlastid transient expression vector-
dc.titleConstruction of plastid transient expression vector of GFP gene and its expression in E. Colien_HK
dc.title綠色熒光蛋白質體瞬間表達載體的構建及在大腸桿菌中的表達-
dc.typeArticleen_HK
dc.identifier.emailSun, M: meisun@hkucc.hku.hken_HK
dc.identifier.authoritySun, M=rp00779en_HK
dc.identifier.hkuros22578en_HK
dc.identifier.volume5-
dc.identifier.issue4-
dc.identifier.spage339-
dc.identifier.epage345-
dc.publisher.placeChina-

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