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Article: Identification of an upstream promoter in the human gonadotropin-releasing hormone receptor gene

TitleIdentification of an upstream promoter in the human gonadotropin-releasing hormone receptor gene
Authors
KeywordsGene regulation
Gonadotrope
Human GnRH receptor
Human reproduction
Promoter
Issue Date2000
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2000, v. 270 n. 3, p. 766-772 How to Cite?
AbstractAnalysis of the human gonadotropin-releasing hormone receptor (hGnRHR) gene 5' flanking region revealed the presence of multiple TATA, CCAAT, and transcription start sites. In addition, at least three different transcripts (5.0, 2.5, and 1.5 kb) were detected by Northern blot analysis. Taken together, these data indicated the existence of multiple promoter elements in the hGnRHR gene, and these promoters are responsible for the multiplicity of regulation of human reproductive functions. In this report, by progressive 5' and 3' deletion (-2197 to -1351, relative to the ATG) and NotI linker scanning mutagenesis coupled to transient transfection into the mouse gonadotrope-derived αT3-1 cell, a distal promoter element was identified at -1705/-1674. The promoter was located immediately 5' to a previously identified CAP site at -1673 in human pituitary and it drove a 17.6- ± 1.0-fold increase in reporter gene activity. Within the promoter, a pyrimidine-rich initiator element (Inr) (-1682) and a CCAAT box (-1702) were found and mutation of these elements abrogated both protein bindings and promoter activities. By 1- and 2-D South-Western blot assays, multiple nuclear factors (40 to 54 kDa) were found to interact specifically with this promoter element. These nuclear factors were also present in other cells, including COS-7, JEG-3, and SKOV-3 cells, and these findings were consistent with functional studies which showed that the promoter is also active in these cells. (C) 2000 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/84699
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNgan, ESWen_HK
dc.contributor.authorLeung, PCKen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:56:05Z-
dc.date.available2010-09-06T08:56:05Z-
dc.date.issued2000en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2000, v. 270 n. 3, p. 766-772en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84699-
dc.description.abstractAnalysis of the human gonadotropin-releasing hormone receptor (hGnRHR) gene 5' flanking region revealed the presence of multiple TATA, CCAAT, and transcription start sites. In addition, at least three different transcripts (5.0, 2.5, and 1.5 kb) were detected by Northern blot analysis. Taken together, these data indicated the existence of multiple promoter elements in the hGnRHR gene, and these promoters are responsible for the multiplicity of regulation of human reproductive functions. In this report, by progressive 5' and 3' deletion (-2197 to -1351, relative to the ATG) and NotI linker scanning mutagenesis coupled to transient transfection into the mouse gonadotrope-derived αT3-1 cell, a distal promoter element was identified at -1705/-1674. The promoter was located immediately 5' to a previously identified CAP site at -1673 in human pituitary and it drove a 17.6- ± 1.0-fold increase in reporter gene activity. Within the promoter, a pyrimidine-rich initiator element (Inr) (-1682) and a CCAAT box (-1702) were found and mutation of these elements abrogated both protein bindings and promoter activities. By 1- and 2-D South-Western blot assays, multiple nuclear factors (40 to 54 kDa) were found to interact specifically with this promoter element. These nuclear factors were also present in other cells, including COS-7, JEG-3, and SKOV-3 cells, and these findings were consistent with functional studies which showed that the promoter is also active in these cells. (C) 2000 Academic Press.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectGene regulationen_HK
dc.subjectGonadotropeen_HK
dc.subjectHuman GnRH receptoren_HK
dc.subjectHuman reproductionen_HK
dc.subjectPromoteren_HK
dc.titleIdentification of an upstream promoter in the human gonadotropin-releasing hormone receptor geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=270&spage=766&epage=772&date=2000&atitle=Identification+of+an+Upstream+Promoter+in+the+Human+Gonadotropin-Releasing+Hormone+Receptor+Geneen_HK
dc.identifier.emailNgan, ESW: engan@hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityNgan, ESW=rp00422en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/bbrc.2000.2509en_HK
dc.identifier.pmid10772899-
dc.identifier.scopuseid_2-s2.0-0034696838en_HK
dc.identifier.hkuros48612en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034696838&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume270en_HK
dc.identifier.issue3en_HK
dc.identifier.spage766en_HK
dc.identifier.epage772en_HK
dc.identifier.isiWOS:000086793200017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNgan, ESW=22234827500en_HK
dc.identifier.scopusauthoridLeung, PCK=55419381000en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK

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