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Article: Characterization of calcium signaling by purinergic receptor-channels expressed in excitable cells

TitleCharacterization of calcium signaling by purinergic receptor-channels expressed in excitable cells
Authors
Issue Date2000
PublisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://www.molpharm.org
Citation
Molecular Pharmacology, 2000, v. 58 n. 5, p. 936-945 How to Cite?
AbstractATP-gated purinergic receptors (P2XRs) are a family of cation-permeable channels that conduct Ca2+ and facilitate voltage-sensitive Ca2+ entry in excitable cells. To study Ca2+ signaling by P2XRs and its dependence on voltage-sensitive Ca2+ influx, we expressed eight cloned P2XR subtypes individually in gonadotropin-releasing hormone-secreting neurons. In all cases, ATP evoked an inward current and a rise in [Ca2+](i). P2XR subtypes differed in the peak amplitude of [Ca2+](i) response independently of the level of receptor expression, with the following order: P2X1R < P2X3R < P2X4R < P2X(2b)R < P2X(2a)R < P2X7R. During prolonged agonist stimulation, Ca2+ signals desensitized with different rates: P2X3R > P2X1R > P2X(2b)R > P2X4R >> P2X(2a)R >> P2X7R. The pattern of [Ca2+](i) response for each P2XR subtype was highly comparable with that of the depolarizing current, but the activation and desensitization rates were faster for the current than for [Ca2+](i). The P2X1R, P2X3R, and P2X4R-derived [Ca2+](i) signals were predominantly dependent on activation of voltage-sensitive Ca2+ influx, both voltage-sensitive and -insensitive Ca2+ entry pathways equally contributed to [Ca2+](i) responses in P2X(2a)R- and P2X(2b)R-expressing cells, and P2X7R operated as a nonselective pore capable of conducting larger amounts of Ca2+ independently on the status of voltage-gated Ca2+ channels. Thus, Ca2+ signaling by homomeric P2XRs expressed in an excitable cell is subtype-specific, which provides an effective mechanism for generating variable [Ca2+](i) patterns in response to a common agonist.
Persistent Identifierhttp://hdl.handle.net/10722/84697
ISSN
2015 Impact Factor: 3.931
2015 SCImago Journal Rankings: 2.047
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKoshimizu, TAen_HK
dc.contributor.authorVan Goor, Fen_HK
dc.contributor.authorTomić, Men_HK
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorTanoue, Aen_HK
dc.contributor.authorTsujimoto, Gen_HK
dc.contributor.authorStojilkovic, SSen_HK
dc.date.accessioned2010-09-06T08:56:04Z-
dc.date.available2010-09-06T08:56:04Z-
dc.date.issued2000en_HK
dc.identifier.citationMolecular Pharmacology, 2000, v. 58 n. 5, p. 936-945en_HK
dc.identifier.issn0026-895Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84697-
dc.description.abstractATP-gated purinergic receptors (P2XRs) are a family of cation-permeable channels that conduct Ca2+ and facilitate voltage-sensitive Ca2+ entry in excitable cells. To study Ca2+ signaling by P2XRs and its dependence on voltage-sensitive Ca2+ influx, we expressed eight cloned P2XR subtypes individually in gonadotropin-releasing hormone-secreting neurons. In all cases, ATP evoked an inward current and a rise in [Ca2+](i). P2XR subtypes differed in the peak amplitude of [Ca2+](i) response independently of the level of receptor expression, with the following order: P2X1R < P2X3R < P2X4R < P2X(2b)R < P2X(2a)R < P2X7R. During prolonged agonist stimulation, Ca2+ signals desensitized with different rates: P2X3R > P2X1R > P2X(2b)R > P2X4R >> P2X(2a)R >> P2X7R. The pattern of [Ca2+](i) response for each P2XR subtype was highly comparable with that of the depolarizing current, but the activation and desensitization rates were faster for the current than for [Ca2+](i). The P2X1R, P2X3R, and P2X4R-derived [Ca2+](i) signals were predominantly dependent on activation of voltage-sensitive Ca2+ influx, both voltage-sensitive and -insensitive Ca2+ entry pathways equally contributed to [Ca2+](i) responses in P2X(2a)R- and P2X(2b)R-expressing cells, and P2X7R operated as a nonselective pore capable of conducting larger amounts of Ca2+ independently on the status of voltage-gated Ca2+ channels. Thus, Ca2+ signaling by homomeric P2XRs expressed in an excitable cell is subtype-specific, which provides an effective mechanism for generating variable [Ca2+](i) patterns in response to a common agonist.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://www.molpharm.orgen_HK
dc.relation.ispartofMolecular Pharmacologyen_HK
dc.titleCharacterization of calcium signaling by purinergic receptor-channels expressed in excitable cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0026-895X&volume=58&spage=936&epage=945&date=2000&atitle=Characterization+of+Calcium+Signaling+by+Purinergic+Receptor-Channels+Expressed+in+Excitable+Cellsen_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11040040-
dc.identifier.scopuseid_2-s2.0-0033789010en_HK
dc.identifier.hkuros58827en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033789010&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume58en_HK
dc.identifier.issue5en_HK
dc.identifier.spage936en_HK
dc.identifier.epage945en_HK
dc.identifier.isiWOS:000089918900008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKoshimizu, TA=26643110900en_HK
dc.identifier.scopusauthoridVan Goor, F=35845505200en_HK
dc.identifier.scopusauthoridTomić, M=7006939182en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK
dc.identifier.scopusauthoridTanoue, A=7004117979en_HK
dc.identifier.scopusauthoridTsujimoto, G=7103184340en_HK
dc.identifier.scopusauthoridStojilkovic, SS=7004268568en_HK

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