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Article: Two Inr elements are important for mediating the activity of the proximal promoter of the human gonadotropin-releasing hormone receptor gene

TitleTwo Inr elements are important for mediating the activity of the proximal promoter of the human gonadotropin-releasing hormone receptor gene
Authors
Issue Date2003
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2003, v. 144 n. 2, p. 518-527 How to Cite?
AbstractDifferential usage of several transcription start sites in the human GnRH receptor gene was evident in human brain and pituitary. To locate the promoter responsible for a cluster of the 3′ CAP sites from -635 to -578 (relative to ATG) found in the pituitary, a proximal promoter element was identified at -677/-558 by 5′ and 3′ deletion mutant analysis. The promoter element drove a 13.1 ± 0.6-fold increase in reporter gene activity in an orientation-dependent manner in the mouse gonadotrope-derived αT3-1 cells. Within the core promoter element, two functional AT-rich Inr motifs, interacting with the same protein factor with different affinities, were identified. By Southwestern blot analysis and competitive gel mobility shift assays, multiple nuclear factors (36-150 kDa) were found to interact specifically with the core promoter element. Interestingly, these nuclear proteins also interacted with a previously identified distal promoter of the human GnRH receptor gene. Taken together, our studies suggested that these two promoters share common protein factors to regulate transcription initiations at two different regions. Additional mechanisms are needed to modulate the efficiencies of individual promoters for developmental and/or tissue-specific regulations.
Persistent Identifierhttp://hdl.handle.net/10722/84693
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHoo, RLCen_HK
dc.contributor.authorNgan, ESWen_HK
dc.contributor.authorLeung, PCKen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:56:01Z-
dc.date.available2010-09-06T08:56:01Z-
dc.date.issued2003en_HK
dc.identifier.citationEndocrinology, 2003, v. 144 n. 2, p. 518-527en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84693-
dc.description.abstractDifferential usage of several transcription start sites in the human GnRH receptor gene was evident in human brain and pituitary. To locate the promoter responsible for a cluster of the 3′ CAP sites from -635 to -578 (relative to ATG) found in the pituitary, a proximal promoter element was identified at -677/-558 by 5′ and 3′ deletion mutant analysis. The promoter element drove a 13.1 ± 0.6-fold increase in reporter gene activity in an orientation-dependent manner in the mouse gonadotrope-derived αT3-1 cells. Within the core promoter element, two functional AT-rich Inr motifs, interacting with the same protein factor with different affinities, were identified. By Southwestern blot analysis and competitive gel mobility shift assays, multiple nuclear factors (36-150 kDa) were found to interact specifically with the core promoter element. Interestingly, these nuclear proteins also interacted with a previously identified distal promoter of the human GnRH receptor gene. Taken together, our studies suggested that these two promoters share common protein factors to regulate transcription initiations at two different regions. Additional mechanisms are needed to modulate the efficiencies of individual promoters for developmental and/or tissue-specific regulations.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsEndocrinology. Copyright © The Endocrine Society.-
dc.subject.meshAnimals-
dc.subject.meshBlotting, Southwestern-
dc.subject.meshCell Line-
dc.subject.meshPromoter Regions, Genetic - genetics-
dc.subject.meshReceptors, LHRH - genetics-
dc.titleTwo Inr elements are important for mediating the activity of the proximal promoter of the human gonadotropin-releasing hormone receptor geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=144&issue=2&spage=518&epage=527&date=2003&atitle=Two+Inr+elements+are+important+for+mediating+the+activity+of+the+proximal+promoter+of+the+human+gonadotropin-releasing+hormone+receptor+geneen_HK
dc.identifier.emailHoo, RLC: rubyhoo@hkucc.hku.hken_HK
dc.identifier.emailNgan, ESW: engan@hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityHoo, RLC=rp01334en_HK
dc.identifier.authorityNgan, ESW=rp00422en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1210/en.2002-220591en_HK
dc.identifier.pmid12538612-
dc.identifier.scopuseid_2-s2.0-0037318545en_HK
dc.identifier.hkuros130930-
dc.identifier.hkuros75829-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037318545&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume144en_HK
dc.identifier.issue2en_HK
dc.identifier.spage518en_HK
dc.identifier.epage527en_HK
dc.identifier.isiWOS:000180633800014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHoo, RLC=6602369766en_HK
dc.identifier.scopusauthoridNgan, ESW=22234827500en_HK
dc.identifier.scopusauthoridLeung, PCK=55419381000en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK

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