File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Impact of Yersinia enterocolitica enteritis on disaccharidase activity and small intestinal morphology in colostrum-deprived newborn piglets

TitleImpact of Yersinia enterocolitica enteritis on disaccharidase activity and small intestinal morphology in colostrum-deprived newborn piglets
Authors
Issue Date1997
PublisherNew Zealand Veterinary Association. The Journal's web site is located at http://www.vetjournal.org.nz
Citation
New Zealand Veterinary Journal, 1997, v. 45 n. 1, p. 27-36 How to Cite?
AbstractThe effect of enteritis on the development of the small intestine was examined in newborn, colostrum-deprived piglets infected with a human isolate of Y. enterocolitica (serotype O:3, biotype 4) soon after birth. The piglets were killed 3 days (n = 6) or 5 days (n = 8) after infection, or antibiotic therapy was commenced on day 5 and the animals killed on day 14 (n = 5). Compared with the non-infected controls, infected animals had reduced mucosal lactase and sucrase, but not maltase activity, while after antibiotic therapy, previously infected piglets had a lower lactase and a higher maltase and sucrase activity. Lactase activity was significantly reduced in the duodenum and jejunum, and mean values were lower in the ileum, but the difference did not reach significance; maltase activity was greater at all ages from the distal jejunum to the mid-ileum; sucrase activity was reduced in all segments up to day 5 but after antibiotic therapy was increased in the jejunum and appeared early in the ileum. Enzyme profiles were more mature along the crypt-villus axis in some segments of the intestine in previously infected piglets. Sodium-potassium-ATPase activity was unchanged. There was a reduced villus height:crypt depth ratio, crypt hyperplasia and increased crypt cell proliferation. Morphological maturation, indicated by loss of vacuoles and location of the nucleus at the base of the enterocyte, proceeded distally from the duodenum to ileum from 3 to 14 days of age when only the ileum remained immature. In infected piglets, there was reduced vacuolation and earlier location of the nucleus at the base of the cell in the distal intestine. Accelerated maturity of specific disaccharidases and enterocyte morphology in infected piglets appears to be due to physical damage to the mucosa resulting in faster proliferation of crypt cells and migration of enterocytes. It is suggested that this may reduce macromolecular internalisation and impair the ability to utilise dietary carbohydrate and may have long-term effects on growth and immunological responses of the gut.
Persistent Identifierhttp://hdl.handle.net/10722/84686
ISSN
2015 Impact Factor: 1.08
2015 SCImago Journal Rankings: 0.568
References

 

DC FieldValueLanguage
dc.contributor.authorShu, Den_HK
dc.contributor.authorSimpson, HVen_HK
dc.contributor.authorXu, RJen_HK
dc.contributor.authorMellor, DJen_HK
dc.contributor.authorReynolds, GWen_HK
dc.contributor.authorMarshall, RBen_HK
dc.date.accessioned2010-09-06T08:55:56Z-
dc.date.available2010-09-06T08:55:56Z-
dc.date.issued1997en_HK
dc.identifier.citationNew Zealand Veterinary Journal, 1997, v. 45 n. 1, p. 27-36en_HK
dc.identifier.issn0048-0169en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84686-
dc.description.abstractThe effect of enteritis on the development of the small intestine was examined in newborn, colostrum-deprived piglets infected with a human isolate of Y. enterocolitica (serotype O:3, biotype 4) soon after birth. The piglets were killed 3 days (n = 6) or 5 days (n = 8) after infection, or antibiotic therapy was commenced on day 5 and the animals killed on day 14 (n = 5). Compared with the non-infected controls, infected animals had reduced mucosal lactase and sucrase, but not maltase activity, while after antibiotic therapy, previously infected piglets had a lower lactase and a higher maltase and sucrase activity. Lactase activity was significantly reduced in the duodenum and jejunum, and mean values were lower in the ileum, but the difference did not reach significance; maltase activity was greater at all ages from the distal jejunum to the mid-ileum; sucrase activity was reduced in all segments up to day 5 but after antibiotic therapy was increased in the jejunum and appeared early in the ileum. Enzyme profiles were more mature along the crypt-villus axis in some segments of the intestine in previously infected piglets. Sodium-potassium-ATPase activity was unchanged. There was a reduced villus height:crypt depth ratio, crypt hyperplasia and increased crypt cell proliferation. Morphological maturation, indicated by loss of vacuoles and location of the nucleus at the base of the enterocyte, proceeded distally from the duodenum to ileum from 3 to 14 days of age when only the ileum remained immature. In infected piglets, there was reduced vacuolation and earlier location of the nucleus at the base of the cell in the distal intestine. Accelerated maturity of specific disaccharidases and enterocyte morphology in infected piglets appears to be due to physical damage to the mucosa resulting in faster proliferation of crypt cells and migration of enterocytes. It is suggested that this may reduce macromolecular internalisation and impair the ability to utilise dietary carbohydrate and may have long-term effects on growth and immunological responses of the gut.en_HK
dc.languageengen_HK
dc.publisherNew Zealand Veterinary Association. The Journal's web site is located at http://www.vetjournal.org.nzen_HK
dc.relation.ispartofNew Zealand Veterinary Journalen_HK
dc.titleImpact of Yersinia enterocolitica enteritis on disaccharidase activity and small intestinal morphology in colostrum-deprived newborn pigletsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0048-0169&volume=45&spage=27&epage=36&date=1997&atitle=Impact+of+Yersinia+enterocolitica+enteritis+on+disaccharidase+activity+and+small+intestinal+morphology+in+colostrum-deprived+newborn+pigletsen_HK
dc.identifier.emailXu, RJ: xuruojun@hkucc.hku.hken_HK
dc.identifier.authorityXu, RJ=rp00820en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-3042982889en_HK
dc.identifier.hkuros21520en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3042982889&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue1en_HK
dc.identifier.spage27en_HK
dc.identifier.epage36en_HK
dc.publisher.placeNew Zealanden_HK
dc.identifier.scopusauthoridShu, D=7103152041en_HK
dc.identifier.scopusauthoridSimpson, HV=7202391355en_HK
dc.identifier.scopusauthoridXu, RJ=7402813973en_HK
dc.identifier.scopusauthoridMellor, DJ=7103269442en_HK
dc.identifier.scopusauthoridReynolds, GW=7201487841en_HK
dc.identifier.scopusauthoridMarshall, RB=7401713880en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats