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Article: Somatostatin inhibits (D-Arg6, Pro9-NEt) salmon gonadotropin-releasing hormone- and dopamine D1-stimulated growth hormone release from perifused pituitary cells of chinese grass carp, Ctenopharyngodon idellus

TitleSomatostatin inhibits (D-Arg6, Pro9-NEt) salmon gonadotropin-releasing hormone- and dopamine D1-stimulated growth hormone release from perifused pituitary cells of chinese grass carp, Ctenopharyngodon idellus
Authors
KeywordsChinese grass carp
D1 receptors
Dopamine
Gonadotropin-releasing hormone
Growth hormone
Pituitary cells
Somatostatin
Issue Date1998
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 1998, v. 110 n. 1, p. 29-45 How to Cite?
AbstractIn this study, a heterologous radioimmunoassay (RIA) for grass carp GH has been validated and used to monitor the kinetics of GH release from perifused grass carp pituitary cells. To establish the anatomical specificity of GH antiserum used in this RIA, immunohistochemical staining was performed in grass carp pituitary sections. Somatotrophs recognized by this GH antiserum were located mainly in the proximal pars distalis without overlapping with gonadotrophs located in the same area or with lactotrophs located in the rostral pars distalis. The immunoreactivity of somatotrophs was abolished by preabsorbing GH antiserum with purified grass carp GH, suggesting that the possibility of a cross-reactivity of antiserum with other grass carp pituitary hormones is unlikely. Using 125I-labeled carp GH as the RIA tracer, parallelism was observed among the displacement curves of grass carp GH standard, grass carp serum, and culture medium conditioned by grass carp pituitary cells, suggesting that this RIA can be used to quantitate grass carp GH levels in biological samples. Using an in vitro column perifusion system, a superactive gonadotropin-releasing hormone (GnRH) analog (D-Arg6, Pro9-NEt)-sGnRH (sGnRHa, 0.3-30 nM), dopamine (DA, 0.1-10 μM), and the nonselective DA agonist apomorphine (0.1-10 μM) stimulated GH release from grass carp pituitary cells in a dose-dependent manner. The GH- releasing effect of DA was mimicked by the D1 agonists SKF38393 (0.1-10 μM) and SKF77434 (0.1-10 μM), but not by the D2 agonist LY171555 (3 μM). In addition, the GH response to DA (1 μM) was blocked by the D1 antagonist SCH23390 (5 μM) but not by the D2 antagonist (±) sulpiride (5 μM), suggesting that the GH-releasing action of DA is mediated through receptors resembling mammalian D1 receptors. Somatostatin-14 (SRIF14, 0.01-100 nM), unlike sGnRHa and DA, induced a dose-dependent suppression on basal GH release. At a high dose (100 nM), SRIF14 also abolished the GH responses to sGnRHa (100 nM), DA (10 μM), and the D1 agonist SKF38393 (3 μM). These results, as a whole, provide evidence that GH release in the grass carp is under the direct regulation of GnRH, DA, and SRIF at the pituitary cell level. The present study also suggests that DA D1 receptors are present in grass carp pituitary cells mediating the GH-releasing action of DA.
Persistent Identifierhttp://hdl.handle.net/10722/84683
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.245
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorNg, Sen_HK
dc.contributor.authorLee, EKYen_HK
dc.contributor.authorLeung, RCYen_HK
dc.contributor.authorHo, WKKen_HK
dc.date.accessioned2010-09-06T08:55:54Z-
dc.date.available2010-09-06T08:55:54Z-
dc.date.issued1998en_HK
dc.identifier.citationGeneral And Comparative Endocrinology, 1998, v. 110 n. 1, p. 29-45en_HK
dc.identifier.issn0016-6480en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84683-
dc.description.abstractIn this study, a heterologous radioimmunoassay (RIA) for grass carp GH has been validated and used to monitor the kinetics of GH release from perifused grass carp pituitary cells. To establish the anatomical specificity of GH antiserum used in this RIA, immunohistochemical staining was performed in grass carp pituitary sections. Somatotrophs recognized by this GH antiserum were located mainly in the proximal pars distalis without overlapping with gonadotrophs located in the same area or with lactotrophs located in the rostral pars distalis. The immunoreactivity of somatotrophs was abolished by preabsorbing GH antiserum with purified grass carp GH, suggesting that the possibility of a cross-reactivity of antiserum with other grass carp pituitary hormones is unlikely. Using 125I-labeled carp GH as the RIA tracer, parallelism was observed among the displacement curves of grass carp GH standard, grass carp serum, and culture medium conditioned by grass carp pituitary cells, suggesting that this RIA can be used to quantitate grass carp GH levels in biological samples. Using an in vitro column perifusion system, a superactive gonadotropin-releasing hormone (GnRH) analog (D-Arg6, Pro9-NEt)-sGnRH (sGnRHa, 0.3-30 nM), dopamine (DA, 0.1-10 μM), and the nonselective DA agonist apomorphine (0.1-10 μM) stimulated GH release from grass carp pituitary cells in a dose-dependent manner. The GH- releasing effect of DA was mimicked by the D1 agonists SKF38393 (0.1-10 μM) and SKF77434 (0.1-10 μM), but not by the D2 agonist LY171555 (3 μM). In addition, the GH response to DA (1 μM) was blocked by the D1 antagonist SCH23390 (5 μM) but not by the D2 antagonist (±) sulpiride (5 μM), suggesting that the GH-releasing action of DA is mediated through receptors resembling mammalian D1 receptors. Somatostatin-14 (SRIF14, 0.01-100 nM), unlike sGnRHa and DA, induced a dose-dependent suppression on basal GH release. At a high dose (100 nM), SRIF14 also abolished the GH responses to sGnRHa (100 nM), DA (10 μM), and the D1 agonist SKF38393 (3 μM). These results, as a whole, provide evidence that GH release in the grass carp is under the direct regulation of GnRH, DA, and SRIF at the pituitary cell level. The present study also suggests that DA D1 receptors are present in grass carp pituitary cells mediating the GH-releasing action of DA.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_HK
dc.relation.ispartofGeneral and Comparative Endocrinologyen_HK
dc.subjectChinese grass carpen_HK
dc.subjectD1 receptorsen_HK
dc.subjectDopamineen_HK
dc.subjectGonadotropin-releasing hormoneen_HK
dc.subjectGrowth hormoneen_HK
dc.subjectPituitary cellsen_HK
dc.subjectSomatostatinen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshApomorphine - antagonists & inhibitorsen_HK
dc.subject.meshCarps - physiologyen_HK
dc.subject.meshDopamine Antagonists - pharmacologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGonadotropin-Releasing Hormone - analogs & derivatives - antagonists & inhibitorsen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMaleen_HK
dc.subject.meshNeurosecretory Systems - physiologyen_HK
dc.subject.meshPerfusionen_HK
dc.subject.meshPituitary Gland - cytology - secretionen_HK
dc.subject.meshRadioimmunoassayen_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.subject.meshSalmonen_HK
dc.subject.meshSomatostatin - physiology - secretionen_HK
dc.titleSomatostatin inhibits (D-Arg6, Pro9-NEt) salmon gonadotropin-releasing hormone- and dopamine D1-stimulated growth hormone release from perifused pituitary cells of chinese grass carp, Ctenopharyngodon idellusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-6480&volume=110&spage=29&epage=45&date=1998&atitle=Somatostatin+Inhibits+(D-Arg6,+Pro9-NEt)+salmon+Gonadotropin-releasing+hormone-+and+dopamine+D1-stimulated+Growth+Hormone+release+from+perifused+pituitary+cells+of+chinese+grass+carp,+Ctenopharyngodon+idellusen_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.emailNg, S: tstng@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.identifier.authorityNg, S=rp00158en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/gcen.1997.7045en_HK
dc.identifier.pmid9514844-
dc.identifier.scopuseid_2-s2.0-0031948268en_HK
dc.identifier.hkuros31186en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031948268&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume110en_HK
dc.identifier.issue1en_HK
dc.identifier.spage29en_HK
dc.identifier.epage45en_HK
dc.identifier.isiWOS:000072857300005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK
dc.identifier.scopusauthoridNg, S=7403358853en_HK
dc.identifier.scopusauthoridLee, EKY=7406968652en_HK
dc.identifier.scopusauthoridLeung, RCY=36729486100en_HK
dc.identifier.scopusauthoridHo, WKK=7402968862en_HK

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