File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Stability and distribution of orally administered epidermal growth factor in neonatal pigs

TitleStability and distribution of orally administered epidermal growth factor in neonatal pigs
Authors
KeywordsEGF
Gastrointestinal development
Milk
Pig
Issue Date1998
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1998, v. 63 n. 10, p. 809-820 How to Cite?
AbstractStability and distribution of orally administered epidermal growth factor (EGF) were examined in newborn and 5-day-old pigs. Forty-five minutes after oral administration of iodine-125 labeled EGF, 60 and 50% of the radioactivity administered were recovered from the internal organs in newborn and 5-day-old pigs, respectively. In both age groups, over 95% of the recovered radioactivity was found in the gastrointestinal tract, of which 78- 86% was found in the luminal contents with the remaining found in the gastrointestinal wall. Within the gastrointestinal tract, 65-71% of radioactivity was found in the stomach, 27-30% in the proximal and mid small intestine and 3-4% was found in the distal part of the small intestine. There were no significant differences in the overall distribution of orally administered radioactivity between two age groups. Based on liquid chromatography and trichloroacetic acid precipitation, a substantial amount of EGF recovered from the luminal contents (63-86%) and the gastrointestinal wall (42 - 81%) remained 'intact'. The receptor binding ability of the EGF recovered from the gastric contents was 96 - 102% comparable to the native EGF tracer. The receptor binding ability remained high (40 - 58%) in the proximal small intestinal lumen and it decreased to 15% in the distal small intestinal lumen in newborn pigs. In 5-day-old pigs, EGF recovered from the small intestinal contents had 5 to 24% receptor binding ability when compared with native EGF tracer. The receptor binding ability of the EGF recovered from all other organs was below 5% with an exception of the gastric wall, from which recovered EGF retained 9 to 26% receptor binding ability. These results indicate that most of orally ingested EGF remained in the gastrointestinal tract in neonatal pigs 45 min after oral ingestion, and significant amount of the ingested EGF remained biologically active. It suggests that milk-borne EGF can survive in the gastrointestinal tract and may play a role in regulating gut development in neonatal animals.
Persistent Identifierhttp://hdl.handle.net/10722/84682
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShen, WHen_HK
dc.contributor.authorXu, RJen_HK
dc.date.accessioned2010-09-06T08:55:53Z-
dc.date.available2010-09-06T08:55:53Z-
dc.date.issued1998en_HK
dc.identifier.citationLife Sciences, 1998, v. 63 n. 10, p. 809-820en_HK
dc.identifier.issn0024-3205en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84682-
dc.description.abstractStability and distribution of orally administered epidermal growth factor (EGF) were examined in newborn and 5-day-old pigs. Forty-five minutes after oral administration of iodine-125 labeled EGF, 60 and 50% of the radioactivity administered were recovered from the internal organs in newborn and 5-day-old pigs, respectively. In both age groups, over 95% of the recovered radioactivity was found in the gastrointestinal tract, of which 78- 86% was found in the luminal contents with the remaining found in the gastrointestinal wall. Within the gastrointestinal tract, 65-71% of radioactivity was found in the stomach, 27-30% in the proximal and mid small intestine and 3-4% was found in the distal part of the small intestine. There were no significant differences in the overall distribution of orally administered radioactivity between two age groups. Based on liquid chromatography and trichloroacetic acid precipitation, a substantial amount of EGF recovered from the luminal contents (63-86%) and the gastrointestinal wall (42 - 81%) remained 'intact'. The receptor binding ability of the EGF recovered from the gastric contents was 96 - 102% comparable to the native EGF tracer. The receptor binding ability remained high (40 - 58%) in the proximal small intestinal lumen and it decreased to 15% in the distal small intestinal lumen in newborn pigs. In 5-day-old pigs, EGF recovered from the small intestinal contents had 5 to 24% receptor binding ability when compared with native EGF tracer. The receptor binding ability of the EGF recovered from all other organs was below 5% with an exception of the gastric wall, from which recovered EGF retained 9 to 26% receptor binding ability. These results indicate that most of orally ingested EGF remained in the gastrointestinal tract in neonatal pigs 45 min after oral ingestion, and significant amount of the ingested EGF remained biologically active. It suggests that milk-borne EGF can survive in the gastrointestinal tract and may play a role in regulating gut development in neonatal animals.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_HK
dc.relation.ispartofLife Sciencesen_HK
dc.rightsLife Sciences. Copyright © Elsevier Inc.en_HK
dc.subjectEGFen_HK
dc.subjectGastrointestinal developmenten_HK
dc.subjectMilken_HK
dc.subjectPigen_HK
dc.titleStability and distribution of orally administered epidermal growth factor in neonatal pigsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0024-3205&volume=63&spage=809&epage=820&date=1998&atitle=Stability+and+distribution+of+orally+administered+epidermal+growth+factor+in+neonatal+pigsen_HK
dc.identifier.emailXu, RJ: xuruojun@hkucc.hku.hken_HK
dc.identifier.authorityXu, RJ=rp00820en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0024-3205(98)00337-3en_HK
dc.identifier.pmid9734700-
dc.identifier.scopuseid_2-s2.0-0032584614en_HK
dc.identifier.hkuros33405en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032584614&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume63en_HK
dc.identifier.issue10en_HK
dc.identifier.spage809en_HK
dc.identifier.epage820en_HK
dc.identifier.isiWOS:000075237200001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShen, WH=46261460000en_HK
dc.identifier.scopusauthoridXu, RJ=7402813973en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats