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Article: Sequence analysis of cDNA gene coding for VP2 of CJ801 strain of infectious bursal disease virus

TitleSequence analysis of cDNA gene coding for VP2 of CJ801 strain of infectious bursal disease virus
傳染性囊病病毒CJ801 VP2基因cDNA的序列分析
Authors
KeywordsInfectious bursal disease virus (IBDV)
Gene cloning
Sequence analysis
Issue Date1997
PublisherSouth China Agricultural University. The Journal's web site is located at http://www.cnki.com.cn/Journal/D-D7-HNNB.htm
Citation
華南農業大學學報, 1997, v. 18, Suppl., p. 65-72 How to Cite?
Journal of South China Agricultural University, 1997, v. 18, Suppl., p. 65-72 How to Cite?
AbstractA cDNA fragment with a length of 1500bp coding for VP2 of infectious bursal disease virus (IBDV) has been amplified by reverse transcription-polymerase chain reaction(RT-PCR)from CJ 801 strain isolated in China. DNA sequence analysis showed that the similarities of nucleotide sequence of CJ801 strain with classical type I strains 52/70, STC and Cul were 97.4%, 97.6% and 96.9% respectively, those with variant strains A, E and GLS were a little smaller, 96.5%, 96.3% and 96.9%,respectively, and those with type Ⅱ strains were about 81%. Cluster analysis of nucleotide sequences indicated that CJ801 strain is more closely related to classical virulent strains and classical tissue culture-adapted strains than to variant strains, very virulent strains and type Ⅱ strains. Comparison of deduced amino acid sequences showed that the homogenneities of CJ 801 strain with all classical type I strains were over 96%.The most closely related strain to CJ801 strain is 52/70 strain, in which the divergence of amino acid sequences was only 1.8%. A serine-rich heptapeptide(S-W-S-A-S-G-S),which is a signature of virulent IBDV strains, is also present in CJ801, indicating that CJ801 is a classical virulent IBDV strain.At positions 253,279 and 284,the amino acid residues of CJ801 strain are Q, D and A respectively, conserved in that of virulent strains . However, in the attenuated strain of CJ801 (CJ801 BKF),the residues at these positions were substituted by H,N and T respectively.There substitutions were commonly found in cell culture adapted IBDV strains. It is suggested that these amino acid residues, 253 Q,279 D and 284 A can be regarded as signature of virulent strains of IBDV in addition to the serine rich heptapeptide.
Persistent Identifierhttp://hdl.handle.net/10722/84680
ISSN

 

DC FieldValueLanguage
dc.contributor.authorCao, Yen_HK
dc.contributor.authorBi, YZen_HK
dc.contributor.authorLeung, FCCen_HK
dc.contributor.authorZhou, Jen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2010-09-06T08:55:52Z-
dc.date.available2010-09-06T08:55:52Z-
dc.date.issued1997en_HK
dc.identifier.citation華南農業大學學報, 1997, v. 18, Suppl., p. 65-72en_HK
dc.identifier.citationJournal of South China Agricultural University, 1997, v. 18, Suppl., p. 65-72-
dc.identifier.issn1001-411Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84680-
dc.description.abstractA cDNA fragment with a length of 1500bp coding for VP2 of infectious bursal disease virus (IBDV) has been amplified by reverse transcription-polymerase chain reaction(RT-PCR)from CJ 801 strain isolated in China. DNA sequence analysis showed that the similarities of nucleotide sequence of CJ801 strain with classical type I strains 52/70, STC and Cul were 97.4%, 97.6% and 96.9% respectively, those with variant strains A, E and GLS were a little smaller, 96.5%, 96.3% and 96.9%,respectively, and those with type Ⅱ strains were about 81%. Cluster analysis of nucleotide sequences indicated that CJ801 strain is more closely related to classical virulent strains and classical tissue culture-adapted strains than to variant strains, very virulent strains and type Ⅱ strains. Comparison of deduced amino acid sequences showed that the homogenneities of CJ 801 strain with all classical type I strains were over 96%.The most closely related strain to CJ801 strain is 52/70 strain, in which the divergence of amino acid sequences was only 1.8%. A serine-rich heptapeptide(S-W-S-A-S-G-S),which is a signature of virulent IBDV strains, is also present in CJ801, indicating that CJ801 is a classical virulent IBDV strain.At positions 253,279 and 284,the amino acid residues of CJ801 strain are Q, D and A respectively, conserved in that of virulent strains . However, in the attenuated strain of CJ801 (CJ801 BKF),the residues at these positions were substituted by H,N and T respectively.There substitutions were commonly found in cell culture adapted IBDV strains. It is suggested that these amino acid residues, 253 Q,279 D and 284 A can be regarded as signature of virulent strains of IBDV in addition to the serine rich heptapeptide.-
dc.languagechien_HK
dc.publisherSouth China Agricultural University. The Journal's web site is located at http://www.cnki.com.cn/Journal/D-D7-HNNB.htmen_HK
dc.relation.ispartof華南農業大學學報en_HK
dc.relation.ispartofJournal of South China Agricultural University-
dc.subjectInfectious bursal disease virus (IBDV)-
dc.subjectGene cloning-
dc.subjectSequence analysis-
dc.titleSequence analysis of cDNA gene coding for VP2 of CJ801 strain of infectious bursal disease virusen_HK
dc.title傳染性囊病病毒CJ801 VP2基因cDNA的序列分析-
dc.typeArticleen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.identifier.hkuros36107en_HK
dc.identifier.volume18, Suppl.-
dc.identifier.spage65-
dc.identifier.epage72-
dc.publisher.placeChina-

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