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Article: Reduced adiposity in bitter melon (Momordica charantia)-fed rats is associated with increased lipid oxidative enzyme activities and uncoupling protein expression

TitleReduced adiposity in bitter melon (Momordica charantia)-fed rats is associated with increased lipid oxidative enzyme activities and uncoupling protein expression
Authors
KeywordsAcyl-CoA dehydrogenase
Adiponectin
Bitter melon
Carnitine palmitoyl transferase-I
Uncoupling proteins
Issue Date2005
PublisherAmerican Society for Nutrition. The Journal's web site is located at http://jn.nutrition.org
Citation
Journal Of Nutrition, 2005, v. 135 n. 11, p. 2517-2523 How to Cite?
AbstractTo further explore the antiobesity effect of freeze-dried bitter melon (BM) juice, activities of mitochondrial lipid oxidative enzymes as well as the expression of uncoupling proteins and their transcription coactivator peroxisome proliferator-activated receptor-γ coactivator-1 α (PGC-1α) were determined in diet-induced obese (DIO) rats. Rats were fed high-fat (HF) diets to induce obesity, and the effect of BM was assessed at doses of 0.75, 1.0, or 1.25% (wt:wt). In a dose-response experiment, BM-supplemented rats had lower energy efficiency (g weight gained/kJ consumed), visceral fat mass, serum glucose, and insulin resistance index, but higher plasma norepinephrine than unsupplemented rats (P < 0.05). Hepatic and skeletal muscle triglyceride concentrations were lower in supplemented HF diet-fed rats than in unsupplemented HF diet-fed rats (P < 0.05). An HF diet supplemented with BM elevated activities of hepatic and muscle mitochondrial carnitine palmitoyl transferase-I (CPT-I) and acyl-CoA dehydrogenase (AD) (P < 0.05). In another experiment, BM (1.0 g/100 g) lowered visceral fat mass but increased serum adiponectin concentration in HF diet-fed rats (P < 0.05). In the final study, rats were fed the HF diet with 0, 1.0 or 1.25% BM. Both groups of BM-supplemented rats had higher uncoupling protein 1 in brown adipose tissue (P < 0.05) and uncoupling protein 3 in red gastrocnemius muscle (P < 0.05), measured by Western blotting and RT-PCR, than the controls. The expression of the transcription coactivator PGC-1α in both tissues was also significantly elevated in the BM-supplemented rats (P < 0.05). The present results suggest that decreased adiposity in BM-supplemented rats may result from lower metabolic efficiency, a consequence of increased lipid oxidation and mitochondrial uncoupling. © 2005 American Society for Nutrition.
Persistent Identifierhttp://hdl.handle.net/10722/84678
ISSN
2015 Impact Factor: 3.74
2015 SCImago Journal Rankings: 2.040
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, LLYen_HK
dc.contributor.authorChen, Qen_HK
dc.contributor.authorGo, AGGen_HK
dc.contributor.authorLam, EKYen_HK
dc.contributor.authorLi, ETSen_HK
dc.date.accessioned2010-09-06T08:55:51Z-
dc.date.available2010-09-06T08:55:51Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Nutrition, 2005, v. 135 n. 11, p. 2517-2523en_HK
dc.identifier.issn0022-3166en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84678-
dc.description.abstractTo further explore the antiobesity effect of freeze-dried bitter melon (BM) juice, activities of mitochondrial lipid oxidative enzymes as well as the expression of uncoupling proteins and their transcription coactivator peroxisome proliferator-activated receptor-γ coactivator-1 α (PGC-1α) were determined in diet-induced obese (DIO) rats. Rats were fed high-fat (HF) diets to induce obesity, and the effect of BM was assessed at doses of 0.75, 1.0, or 1.25% (wt:wt). In a dose-response experiment, BM-supplemented rats had lower energy efficiency (g weight gained/kJ consumed), visceral fat mass, serum glucose, and insulin resistance index, but higher plasma norepinephrine than unsupplemented rats (P < 0.05). Hepatic and skeletal muscle triglyceride concentrations were lower in supplemented HF diet-fed rats than in unsupplemented HF diet-fed rats (P < 0.05). An HF diet supplemented with BM elevated activities of hepatic and muscle mitochondrial carnitine palmitoyl transferase-I (CPT-I) and acyl-CoA dehydrogenase (AD) (P < 0.05). In another experiment, BM (1.0 g/100 g) lowered visceral fat mass but increased serum adiponectin concentration in HF diet-fed rats (P < 0.05). In the final study, rats were fed the HF diet with 0, 1.0 or 1.25% BM. Both groups of BM-supplemented rats had higher uncoupling protein 1 in brown adipose tissue (P < 0.05) and uncoupling protein 3 in red gastrocnemius muscle (P < 0.05), measured by Western blotting and RT-PCR, than the controls. The expression of the transcription coactivator PGC-1α in both tissues was also significantly elevated in the BM-supplemented rats (P < 0.05). The present results suggest that decreased adiposity in BM-supplemented rats may result from lower metabolic efficiency, a consequence of increased lipid oxidation and mitochondrial uncoupling. © 2005 American Society for Nutrition.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Nutrition. The Journal's web site is located at http://jn.nutrition.orgen_HK
dc.relation.ispartofJournal of Nutritionen_HK
dc.subjectAcyl-CoA dehydrogenaseen_HK
dc.subjectAdiponectinen_HK
dc.subjectBitter melonen_HK
dc.subjectCarnitine palmitoyl transferase-Ien_HK
dc.subjectUncoupling proteinsen_HK
dc.titleReduced adiposity in bitter melon (Momordica charantia)-fed rats is associated with increased lipid oxidative enzyme activities and uncoupling protein expressionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3166&volume=135&spage=2517&epage=2523&date=2005&atitle=Reduced+Adiposity+in+Bitter+Melon+(Momordica+charantia)+Fed+Rats+Is+Associated+with+Increased+Lipid+Oxidative+Enzyme+Activities+and+Uncoupling+Protein+Expression.en_HK
dc.identifier.emailLi, ETS: etsli@hku.hken_HK
dc.identifier.authorityLi, ETS=rp00737en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid16251604-
dc.identifier.scopuseid_2-s2.0-31544471395en_HK
dc.identifier.hkuros112693en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-31544471395&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume135en_HK
dc.identifier.issue11en_HK
dc.identifier.spage2517en_HK
dc.identifier.epage2523en_HK
dc.identifier.isiWOS:000233260000005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, LLY=36907180600en_HK
dc.identifier.scopusauthoridChen, Q=55209782600en_HK
dc.identifier.scopusauthoridGo, AGG=12040738300en_HK
dc.identifier.scopusauthoridLam, EKY=55225431600en_HK
dc.identifier.scopusauthoridLi, ETS=14018169600en_HK

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