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Article: Preparation and characterisation of two monoclonal antibodies against oxidatively modified low-density lipoprotein (O-LDL) and detection of O-LDL in serum in patients with coronary heart disease

TitlePreparation and characterisation of two monoclonal antibodies against oxidatively modified low-density lipoprotein (O-LDL) and detection of O-LDL in serum in patients with coronary heart disease
Authors
KeywordsAtherosclerosis
Coronary heart disease
Monoclonal antibody
Oxidatively modified low-density lipoprotein
Issue Date1997
PublisherChapman & Hall, Journals Department. The Journal's web site is located at http://www.chaphall.com/chaphall/journals.html
Citation
Medical Science Research, 1997, v. 25 n. 7, p. 445-449 How to Cite?
AbstractThere is much evidence that oxidatively modified low-density lipoprotein (O-LDL) plays an important role in the pathogenesis of atherosclerosis. To investigate this further and to facilitate early diagnosis of atherosclerosis, we raised two monoclonal antibodies (mAbs) against O-LDL. Competitive assay showed that one of these, HOL3, recognised O-LDL oxidatively modified for 2, 8 and 16 h. The other, HOL5, interacted only with O-LDL oxidatively modified for 8 and 16 h. The immunological reactivities of the mAbs with serum of healthy donors indicated that the reactivity of HOL3 was stronger than that of HOL5, Compared with healthy donors, the corresponding optical density (OD) values of O-LDL in serum of coronary heart disease patients produced by the two mAbs were all higher, but the degrees of the increase were different. The increase in percentage of serum O-LDL detected with HOL5 in the CHD patients was 337%, while that with HOL3 was only 37%. Therefore, the mAbs, especially HOL5, may be useful tools for investigating O-LDL in the circulation of CHD patients. Because lipid peroxidation and subsequent modification of LDL also occur in some other diseases, these immunological reagents could also be useful in studies on their pathogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/84668
ISSN
2001 Impact Factor: 0.384
2003 SCImago Journal Rankings: 0.107
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Men_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorYan, Den_HK
dc.contributor.authorZhao, Men_HK
dc.contributor.authorLiu, Sen_HK
dc.contributor.authorPang, Zen_HK
dc.contributor.authorWan, Jen_HK
dc.date.accessioned2010-09-06T08:55:43Z-
dc.date.available2010-09-06T08:55:43Z-
dc.date.issued1997en_HK
dc.identifier.citationMedical Science Research, 1997, v. 25 n. 7, p. 445-449en_HK
dc.identifier.issn0269-8951en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84668-
dc.description.abstractThere is much evidence that oxidatively modified low-density lipoprotein (O-LDL) plays an important role in the pathogenesis of atherosclerosis. To investigate this further and to facilitate early diagnosis of atherosclerosis, we raised two monoclonal antibodies (mAbs) against O-LDL. Competitive assay showed that one of these, HOL3, recognised O-LDL oxidatively modified for 2, 8 and 16 h. The other, HOL5, interacted only with O-LDL oxidatively modified for 8 and 16 h. The immunological reactivities of the mAbs with serum of healthy donors indicated that the reactivity of HOL3 was stronger than that of HOL5, Compared with healthy donors, the corresponding optical density (OD) values of O-LDL in serum of coronary heart disease patients produced by the two mAbs were all higher, but the degrees of the increase were different. The increase in percentage of serum O-LDL detected with HOL5 in the CHD patients was 337%, while that with HOL3 was only 37%. Therefore, the mAbs, especially HOL5, may be useful tools for investigating O-LDL in the circulation of CHD patients. Because lipid peroxidation and subsequent modification of LDL also occur in some other diseases, these immunological reagents could also be useful in studies on their pathogenesis.en_HK
dc.languageengen_HK
dc.publisherChapman & Hall, Journals Department. The Journal's web site is located at http://www.chaphall.com/chaphall/journals.htmlen_HK
dc.relation.ispartofMedical Science Researchen_HK
dc.subjectAtherosclerosisen_HK
dc.subjectCoronary heart diseaseen_HK
dc.subjectMonoclonal antibodyen_HK
dc.subjectOxidatively modified low-density lipoproteinen_HK
dc.titlePreparation and characterisation of two monoclonal antibodies against oxidatively modified low-density lipoprotein (O-LDL) and detection of O-LDL in serum in patients with coronary heart diseaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0269-8951&volume=25&spage=445&epage=449&date=1997&atitle=Preparation+and+characterization+of+two+monoclonal+antibodies+against+oxidatively+modified+low-density+lipoprotein+(O-LDL)+and+detection+of+O-LDL+in+serum+in+patients+with+coronary+heart+diseaseen_HK
dc.identifier.emailWan, J: jmfwan@hku.hken_HK
dc.identifier.authorityWan, J=rp00798en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0030857964en_HK
dc.identifier.hkuros35645en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030857964&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue7en_HK
dc.identifier.spage445en_HK
dc.identifier.epage449en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhou, M=7403506134en_HK
dc.identifier.scopusauthoridChen, Y=16745998900en_HK
dc.identifier.scopusauthoridYan, D=55065544200en_HK
dc.identifier.scopusauthoridZhao, M=7403535102en_HK
dc.identifier.scopusauthoridLiu, S=7409463469en_HK
dc.identifier.scopusauthoridPang, Z=7103343225en_HK
dc.identifier.scopusauthoridWan, J=8930305000en_HK

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