File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Genetic authentication of ginseng and other traditional Chinese medicine

TitleGenetic authentication of ginseng and other traditional Chinese medicine
Authors
KeywordsAmplified fragment length polymorphism
Chinese traditional medicine
Ginseng
Microsatellite
Random amplified polymorphic DNA technique
Restriction fragment length polymorphism
Saponins
Issue Date2003
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.html
Citation
Acta Pharmacologica Sinica, 2003, v. 24 n. 9, p. 841-846 How to Cite?
AbstractThe main objective of this paper is to review the chemical and genetic methods used in authentication of ginseng, especially the recent advances in microsatellite genotyping and its application to the authentication of other traditional Chinese medicines (TCM). The standardization and modernization of TCM hinge on the authentication of their botanical identities. Analysis of well-characterized marker compounds is now the most popular method for identifying the herbal materials and quality control of TCM, eg, ginsenoside profiling for authentication of Panax species. However, in many herbal species the chemical composition of the plant changes with the external environment and processing conditions, which lowers the reliability of these authentication methods. In the light of the advances in molecular biotechnology in the past few decades, genetic tools are now considered to provide more standardized and reliable methods for authentication of herbal materials at the DNA level. These genetic tools include random amplified polymorphic DNA (RAPD), DNA fingerprinting using multi-loci probes, restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), and microsatellite marker technology. The practicality of these methods varies in terms of their sensitivity, reliability, reproducibility, and running cost. Using ginseng as an example, we reviewed the advantages and limitations of these molecular techniques in TCM authentication. We have developed a set of microsatellite markers from American ginseng that are able to differentiate Panax ginseng and Panax quinquetolius with the resolution down to farm level, ie, confirmation of its botanical identity and origin. Compared with other molecular techniques, microsatellite marker technology is more robust, accurate, reproducible, reliable, and sensitive. This is essential for large-scale TCM authentication centers.
Persistent Identifierhttp://hdl.handle.net/10722/84660
ISSN
2015 Impact Factor: 3.166
2015 SCImago Journal Rankings: 1.161
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHon, CCen_HK
dc.contributor.authorChow, YCen_HK
dc.contributor.authorZeng, FYen_HK
dc.contributor.authorLeung, FCCen_HK
dc.date.accessioned2010-09-06T08:55:38Z-
dc.date.available2010-09-06T08:55:38Z-
dc.date.issued2003en_HK
dc.identifier.citationActa Pharmacologica Sinica, 2003, v. 24 n. 9, p. 841-846en_HK
dc.identifier.issn1671-4083en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84660-
dc.description.abstractThe main objective of this paper is to review the chemical and genetic methods used in authentication of ginseng, especially the recent advances in microsatellite genotyping and its application to the authentication of other traditional Chinese medicines (TCM). The standardization and modernization of TCM hinge on the authentication of their botanical identities. Analysis of well-characterized marker compounds is now the most popular method for identifying the herbal materials and quality control of TCM, eg, ginsenoside profiling for authentication of Panax species. However, in many herbal species the chemical composition of the plant changes with the external environment and processing conditions, which lowers the reliability of these authentication methods. In the light of the advances in molecular biotechnology in the past few decades, genetic tools are now considered to provide more standardized and reliable methods for authentication of herbal materials at the DNA level. These genetic tools include random amplified polymorphic DNA (RAPD), DNA fingerprinting using multi-loci probes, restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), and microsatellite marker technology. The practicality of these methods varies in terms of their sensitivity, reliability, reproducibility, and running cost. Using ginseng as an example, we reviewed the advantages and limitations of these molecular techniques in TCM authentication. We have developed a set of microsatellite markers from American ginseng that are able to differentiate Panax ginseng and Panax quinquetolius with the resolution down to farm level, ie, confirmation of its botanical identity and origin. Compared with other molecular techniques, microsatellite marker technology is more robust, accurate, reproducible, reliable, and sensitive. This is essential for large-scale TCM authentication centers.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.htmlen_HK
dc.relation.ispartofActa Pharmacologica Sinicaen_HK
dc.subjectAmplified fragment length polymorphismen_HK
dc.subjectChinese traditional medicineen_HK
dc.subjectGinsengen_HK
dc.subjectMicrosatelliteen_HK
dc.subjectRandom amplified polymorphic DNA techniqueen_HK
dc.subjectRestriction fragment length polymorphismen_HK
dc.subjectSaponinsen_HK
dc.titleGenetic authentication of ginseng and other traditional Chinese medicineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1671-4083&volume=24&spage=841&epage=846&date=2003&atitle=Genetic+Authentication+of+Ginseng+and+Other+Traditional+Chinese+Medicineen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12956929en_HK
dc.identifier.scopuseid_2-s2.0-0041817728en_HK
dc.identifier.hkuros93604en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0041817728&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue9en_HK
dc.identifier.spage841en_HK
dc.identifier.epage846en_HK
dc.identifier.isiWOS:000185357500001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHon, CC=7003617137en_HK
dc.identifier.scopusauthoridChow, YC=8049254300en_HK
dc.identifier.scopusauthoridZeng, FY=7202911544en_HK
dc.identifier.scopusauthoridLeung, FCC=7103078633en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats