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Article: Molecular characterization of seven Chinese isolates of infectious bursal disease virus: Classical, very virulent, and variant strains

TitleMolecular characterization of seven Chinese isolates of infectious bursal disease virus: Classical, very virulent, and variant strains
Authors
KeywordsChicken
Chinese
IBDV
Sequences
VP2
Issue Date1998
PublisherAmerican Association of Avian Pathologists, Inc. The Journal's web site is located at http://avdi.allenpress.com/avdionline/?request=index-html
Citation
Avian Diseases, 1998, v. 42 n. 2, p. 340-351 How to Cite?
AbstractSeven infectious bursal disease virus (IBDV) strains isolated from China have been characterized in this study, including a classical strain CJ801, an attenuated strain GZ911, a variant strain GZ902, and four very virulent strains G9201, G9302, F9502, and HK46. With the use of reverse transcription-polymerase chain reaction, the full-length VP2 genes were amplified and the hypervariable regions were sequenced. Protein sequences of the hypervariable region (a.a. 143-382) of the field isolates confirmed their identities. CJ801 has the highest identity to the classical strains STC and 52/70. GZ902 has the highest identity to the American variant strains A, E, and GLS, and they share unique amino acid residue at positions 249K and 254S, which are not present in standard serotype 1 strains. Attenuated strain GZ911, like other cell culture-adapted strains, has substitutions at positions 279(D to N) and 284(A to T) as well as in the serine-rich heptapeptide region. Hence, these substitutions may take an important role in the reduced virulence of these strains. The four very virulent strains have the highest identity to the European very virulent strain UK661 and Japanese strain OKYM. These strains share unique amino acid residues at positions 222A, 2561, and 2941, which are not present in other less virulent strains. The very virulent strains isolated in Guangdong (G9201, G9303) and Fujian (F9502) Provinces have one to five amino acid substitutions at the two hydrophilic domains of VP2 comparing with UK661 and OKYM, indicating that new very virulent strains are evolving. Phylogenetic analysis suggests that Chinese very virulent IBDVs and European very virulent strains are derived from similar origin.
Persistent Identifierhttp://hdl.handle.net/10722/84648
ISSN
2015 Impact Factor: 1.104
2015 SCImago Journal Rankings: 0.772
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCao, YCen_HK
dc.contributor.authorYeung, WSen_HK
dc.contributor.authorLaw, Men_HK
dc.contributor.authorBi, YZen_HK
dc.contributor.authorLeung, FCen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2010-09-06T08:55:30Z-
dc.date.available2010-09-06T08:55:30Z-
dc.date.issued1998en_HK
dc.identifier.citationAvian Diseases, 1998, v. 42 n. 2, p. 340-351en_HK
dc.identifier.issn0005-2086en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84648-
dc.description.abstractSeven infectious bursal disease virus (IBDV) strains isolated from China have been characterized in this study, including a classical strain CJ801, an attenuated strain GZ911, a variant strain GZ902, and four very virulent strains G9201, G9302, F9502, and HK46. With the use of reverse transcription-polymerase chain reaction, the full-length VP2 genes were amplified and the hypervariable regions were sequenced. Protein sequences of the hypervariable region (a.a. 143-382) of the field isolates confirmed their identities. CJ801 has the highest identity to the classical strains STC and 52/70. GZ902 has the highest identity to the American variant strains A, E, and GLS, and they share unique amino acid residue at positions 249K and 254S, which are not present in standard serotype 1 strains. Attenuated strain GZ911, like other cell culture-adapted strains, has substitutions at positions 279(D to N) and 284(A to T) as well as in the serine-rich heptapeptide region. Hence, these substitutions may take an important role in the reduced virulence of these strains. The four very virulent strains have the highest identity to the European very virulent strain UK661 and Japanese strain OKYM. These strains share unique amino acid residues at positions 222A, 2561, and 2941, which are not present in other less virulent strains. The very virulent strains isolated in Guangdong (G9201, G9303) and Fujian (F9502) Provinces have one to five amino acid substitutions at the two hydrophilic domains of VP2 comparing with UK661 and OKYM, indicating that new very virulent strains are evolving. Phylogenetic analysis suggests that Chinese very virulent IBDVs and European very virulent strains are derived from similar origin.en_HK
dc.languageengen_HK
dc.publisherAmerican Association of Avian Pathologists, Inc. The Journal's web site is located at http://avdi.allenpress.com/avdionline/?request=index-htmlen_HK
dc.relation.ispartofAvian Diseasesen_HK
dc.subjectChickenen_HK
dc.subjectChineseen_HK
dc.subjectIBDVen_HK
dc.subjectSequencesen_HK
dc.subjectVP2en_HK
dc.titleMolecular characterization of seven Chinese isolates of infectious bursal disease virus: Classical, very virulent, and variant strainsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0005-2086&volume=42&spage=340&epage=351&date=1998&atitle=Molecular+characterization+of+seven+chinese+isolates+of+infectious+bursal+disease+virus:+classical,+very+virulent,+and+variant+strainsen_HK
dc.identifier.emailYeung, WS: wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WS=rp00331en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9645325-
dc.identifier.scopuseid_2-s2.0-0032052080en_HK
dc.identifier.hkuros34154en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032052080&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue2en_HK
dc.identifier.spage340en_HK
dc.identifier.epage351en_HK
dc.identifier.isiWOS:000074354500014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCao, YC=25632098400en_HK
dc.identifier.scopusauthoridYeung, WS=7102370745en_HK
dc.identifier.scopusauthoridLaw, M=14719699100en_HK
dc.identifier.scopusauthoridBi, YZ=7102642868en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK

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